FastQCFastQC Report
Fri 10 Feb 2023
EGAF00001073684

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001073684
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55578730
Sequences flagged as poor quality0
Sequence length250
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTA618770.11133215890323511TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT557870.10037472968525908TruSeq Adapter, Index 13 (97% over 40bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)