FastQCFastQC Report
Wed 8 May 2024
EGAF00001082969

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001082969
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1752405
Total Bases52.5 Mbp
Sequences flagged as poor quality0
Sequence length30
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTATGCCGTCTTCTGCTTGAAAAAA27642315.773922124166504Illumina Paired End PCR Primer 2 (100% over 24bp)
TATTGCACTTGTCCCGGCCTGTATCTCGTA1638549.35023581877477No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGT835044.7651085222879415No Hit
TACCCTGTAGATCCGAATTTGTATCTCGTA319121.821040227572964No Hit
TATTGCACTTGTCCCGGCCTGTAAATCTCG319111.8209831631386582No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGT248621.418735965715688No Hit
TCGTTTCCGGCTCGAAGGACCAATCTCGTA222581.2701401787828728No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGT212181.2107931671046361No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTA184821.0546648748434295No Hit
TATTGCACTTGTCCCGGCCTGTATATCTCG179471.0241354024897211No Hit
TATTGCACTTGTCCCGGCCTGTAGATCTCG178481.0184860234934277No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTA176321.0061601056833323No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAA143460.8186463745538275Illumina Paired End PCR Primer 2 (100% over 23bp)
GTTTGTGATGACTTACATGGAATCTCGTAT122630.6997811578944365No Hit
AACATTCAACGCTGTCGGTGAGTATCTCGT102540.5851387093736893No Hit
CAAAGTGCTTACAGTGCAGGTAGATCTCGT84420.48173795441122347No Hit
TATTGCACTTGTCCCGGCCTGTTATCTCGT82680.47180874284198No Hit
GTAGTGTTTCCTACTTTATGGAATCTCGTA81680.4661022994113803No Hit
GTAGTGTTTCCTACTTTATGGATCTCGTAT80700.46050998484939265No Hit
CACCCGTAGAACCGACCTTGCGATCTCGTA79680.454689412550181No Hit
TAAAGTGCTTATAGTGCAGGTAGATCTCGT73810.42119258961256106No Hit
TATTGCACTTGTCCCGGCCTGTAATATCTC68850.39288863019678666No Hit
TAGCTTATCAGACTGATGTTGAATCTCGTA67150.38318767636476725No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTA66050.37691058859110765No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTA65190.3720030472407919No Hit
AACATTCAACGCTGTCGGTGAGATCTCGTA63450.36207383567154855No Hit
TCGTTTCCCGGCCAATGCACCAATCTCGTA62900.3589352917847187No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTA61360.35014736890159526No Hit
TCGTACCGTGAGTAATAATGCGATCTCGTA56860.3244683734638968No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTA54690.31208539121949547No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCATC54630.3117430046136595No Hit
TATTGCACTTGTCCCGGCCTGTGAATCTCG53650.30615069005167184No Hit
TACAGTACTGTGATAACTGAATATCTCGTA53590.30580830344583587No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTA53090.30295508173053604No Hit
CAAAGTGCTGTTCGTGCAGGTAGAATCTCG51530.29405302997880056No Hit
TATTGCACTTGTCCCGGCCTGTAAAATCTC50760.28965906853723883No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTA48960.27938747036215944No Hit
TGTAGTGTTTCCTACTTTATGGATCTCGTA43980.25096938207777314No Hit
ACCCTGTAGATCCGAATTTGTATCTCGTAT43840.25017047999748915No Hit
CCCCCCACTGCTAAATTTGACTGGATCTCG42040.2398988818224098No Hit
AACATTCAACGCTGTCGGTGAGAATCTCGT40330.23014086355608435No Hit
TACAGTACTGTGATAACTGAATCTCGTATG38340.218785041129191No Hit
ACTTAACTTGACCGCTCTGACCAATCTCGT37500.21399162864748733No Hit
TGAGGTAGTAGGTTGTGTGGTTATCTCGTA36230.20674444549062576No Hit
TGTAAACATCCTTGACTGGAAGCATCTCGT33020.1884267620784008No Hit
TGAGAACTGAATTCCATAGGCTGTATCTCG33000.18831263320978883No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC30580.17450304010773765No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTAT30310.17296230038147573No Hit
TTCTCACTACTGCACTTGACTAGATCTCGT28960.16525860175016618No Hit
ACCATCGACCGTTGATTGTACCATCTCGTA28900.16491621514433022No Hit
TCGTATCCCACTTCTGACACCAATCTCGTA27980.15966628718817852No Hit
CTTCTCACTACTGCACTTGACTAGATCTCG27150.15492993914078082No Hit
TGTCAGTTTGTCAAATACCCCAATCTCGTA27040.15430223036341484No Hit
CGCGACCTCAGATCAGACGTGGCATCTCGT25930.14796807815544924No Hit
CAAAGTGCTTACAGTGCAGGTAGAATCTCG25760.14699798277224727No Hit
ACTGCAGTGAAGGCACTTGTAGATCTCGTA25540.14574256521751536No Hit
TATTGCACTTGTCCCGGCCTGTTAATCTCG25440.1451719208744554No Hit
TGAGGTAGTAGGTTGTATGGTTATCTCGTA25080.1431176012394395No Hit
CCCCCACTGCTAAATTTGACTGGATCTCGT24730.14112034603872964No Hit
GGGGGTATAGCTCAGTGGAGAGCATTTGAC24710.14100621717011763No Hit
ACCCTGTAGATCCGAATTTGTGAATCTCGT24430.13940841300954973No Hit
TGAGGTAGTAGGTTGTGTGGTTAATCTCGT24120.13763941554606385No Hit
TATTGCACTTGTCCCGGCCTGTAGAATCTC23760.13558509591104798No Hit
TTAATGCTAATCGTGATAGGGGTATCTCGT23360.1333025185388081No Hit
TAGGTAGTTTCCTGTTGTTGGATCTCGTAT23330.1331313252358901No Hit
AAGCGTTGGATTGTTCACCCATCTCGTATG22730.12970745917753032No Hit
CAACTTAACTTGACCGCTCTGACCAATCTC22520.1285091060571044No Hit
CGCGACCTCAGATCAGACGTGGATCTCGTA22360.12759607510820845No Hit
TAGCTTATCAGACTGATGTTGACATCTCGT22060.12588414207902854No Hit
TTAATGCTAATCGTGATAGGGGATCTCGTA22010.12559881990749855No Hit
TCGTTTCCCGGCCCATGCACCAATCTCGTA20440.11663970372145709No Hit
GTACAGTACTGTGATAACTGAATCTCGTAT20310.11589786607547914No Hit
TGTTTGTGATGACTTACATGGAATCTCGTA20260.11561254390394915No Hit
CACCCGTAGAACCGACCTTGCATCTCGTAT20180.11515602842950116No Hit
CTTTCAGTCGGATGTTTACAGATCTCGTAT20160.11504189956088919No Hit
TGAGGTAGTAGGTTGTATAGTTAATCTCGT20100.1146995129550532No Hit
TATTGCACTTGTCCCGGCCTGTTTATCTCG19840.11321583766309729No Hit
AGAATTCACCAAGCGTTGGATTGTTATCTC19760.11275932218864931No Hit
AACTTAACTTGACCGCTCTGACCAATCTCG19650.11213161341128335No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGC19550.1115609690682234No Hit
CGGGACTGACCTGAAATGAAGAGAATAATC19350.11041968038210345No Hit
TATTGCACTTGTCCCGGCCTGTAGTATCTC18760.10705287875804965No Hit
CGCGACCTCAGATCAGACGTGGCGATCTCG18670.10653929884929568No Hit
CATTGCACTTGTCTCGGTCTGTATCTCGTA18610.1061969122434597No Hit
TCGTATCCCACTCCTGACACCAATCTCGTA18460.10534094572886975No Hit
TGAGAACTGAATTCCATAGGCTGATCTCGT18450.10528388129456376No Hit
GTGGGCACATTACCCGTCTGACCATCTCGT18310.1044849792142798No Hit
CGGGACTGACCTGAAATGAAGAGAATACAT18210.10391433487121984No Hit
AACTTGACCGCTCTGACCAATCTCGTATGC18170.10368607713399586No Hit
AACTCGTATGCCGTCTTCTGCTTGAAAAAA17990.10265891731648792Illumina Single End Adapter 1 (95% over 24bp)
CACTAGATTGTGAGCTCCTGGAATCTCGTA17850.10186001523620396No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)