FastQCFastQC Report
Wed 8 May 2024
EGAF00001082970

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001082970
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11956446
Total Bases358.6 Mbp
Sequences flagged as poor quality0
Sequence length30
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTATGCCGTCTTCTGCTTGAAAAAA137281711.481814913896654Illumina Paired End PCR Primer 2 (100% over 24bp)
TGAGATGAAGCACTGTAGCTCTATCTCGTA131726111.01716178871213No Hit
TAGCTTATCAGACTGATGTTGAATCTCGTA7111375.947728948886651No Hit
TTTGTTCGTTCGGCTCGCGTGAATCTCGTA5645834.7219968207944065No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTA4911044.107441291500836No Hit
TGAGATGAAGCACTGTAGCTATCTCGTATG4104043.432491561455637No Hit
TGACCTATGAATTGACAGCCAGATCTCGTA3663583.0641045006183276No Hit
TACCCTGTAGAACCGAATTTGTATCTCGTA3209972.6847191882939128No Hit
TGAGATGAAGCACTGTAGCTCATCTCGTAT2655882.2212955254429283No Hit
TACCCTGTAGATCCGAATTTGTATCTCGTA2038341.7048042537054906No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTA1914741.60142905341604No Hit
TGTAACAGCAACTCCATGTGGAATCTCGTA1871771.565490280305703No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGT1692711.4157300589155006No Hit
TCAGTGCACTACAGAACTTTGATCTCGTAT1617451.3527849329140114No Hit
CTGACCTATGAATTGACAGCCATCTCGTAT966490.8083422113895717No Hit
TAGCTTATCAGACTGATGTTGACATCTCGT901010.7535767735663257No Hit
TAATACTGCCGGGTAATGATGGAATCTCGT897180.7503734805476477No Hit
TGAGATGAAGCACTGTAGCTCTTATCTCGT627930.5251811449656528No Hit
TACCCTGTAGAACCGAATTTGTGATCTCGT623260.5212753020420952No Hit
TGAGATGAAGCACTGTAGCTCAATCTCGTA595090.4977147891605917No Hit
CTGACCTATGAATTGACAGCCAATCTCGTA583740.48822200175537117No Hit
TATTGCACTTGTCCCGGCCTGTATCTCGTA566520.47381972870533595No Hit
TGTAAACATCCTCGACTGGAAGATCTCGTA547880.4582298117684804No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTA546190.45681634826937706No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGT523830.4381151388966253No Hit
TAGCTTATCAGACTGATGTTGATCTCGTAT488090.4082233131818602No Hit
GTCTACGGCCATACCACCCTGAACGCGCCC473680.3961712368374348No Hit
TAGCACCATCTGAAATCGGTTATCTCGTAT473230.3957948708169635No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTA453200.3790424010613187No Hit
TGAGATGAAGCACTGTAGCTCTAATCTCGT444070.3714063526904232No Hit
GTGAAATGTTTAGGACCACTAGATCTCGTA441870.36956634103478575No Hit
TCAGTGCACTACAGAACTTTGAATCTCGTA429440.35917027518043404No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAA377100.3153947251549499Illumina Paired End PCR Primer 2 (100% over 23bp)
CACTAGATTGTGAGCTCCTGGAATCTCGTA358740.30003899151972085No Hit
TTTGTTCGTTCGGCTCGCGTGATCTCGTAT339750.28415634545583196No Hit
TACAGTACTGTGATAACTGAATCTCGTATG310410.25961728092110314No Hit
TGTAACAGCAACTCCATGTGGAAATCTCGT309540.258889639948192No Hit
CACCCGTAGAACCGACCTTGCGATCTCGTA299130.2501830393412892No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTA295180.2468793820504856No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTA287880.24077388882950668No Hit
TGAGATGAAGCACTGTAGCTAATCTCGTAT285980.23918478785418343No Hit
TAATACTGCCGGGTAATGATGGATCTCGTA281570.23549640085356469No Hit
TACAGTACTGTGATAACTGAATATCTCGTA280640.23471857774459065No Hit
ACCCTGTAGATCCGAATTTGTATCTCGTAT273910.22908981481620877No Hit
GTCCAGTTTTCCCAGGAATCCCTATCTCGT266630.2230010489739175No Hit
AACCCGTAGATCCGAACTTGTGAATCTCGT258280.21601736837183894No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTAT236190.19754197861136996No Hit
TGAGATGAAGCACTGTAGATCTCGTATGCC214770.17962695603693607No Hit
ATGACCTATGAATTGACAGACAATCTCGTA212920.17807967350833181No Hit
ACCCTGTAGAACCGAATTTGTGATCTCGTA205770.17209963562751004No Hit
ACTGGACTTGGAGTCAGAAGGCATCTCGTA205250.1716647237816321No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC202650.16949016455224236No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTA202120.1690468890170206No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCAT198440.16596905133849976No Hit
ACCCTGTAGATCCGAATTTGTGAATCTCGT198090.1656763222114665No Hit
TGACCTATGAATTGACAGCCAGTATCTCGT194970.16306685113619884No Hit
TACCCTGTAGAACCGAATTTGATCTCGTAT194770.1628995773493227No Hit
GTACAGTACTGTGATAACTGAATCTCGTAT191190.15990537656423992No Hit
TGAAATGTTTAGGACCACTAGATCTCGTAT190320.15917773559132872No Hit
GCGGCGACTCTGGACGCGAGCATCTCGTAT184660.15444388742273416No Hit
TGTAACAGCAACTCCATGTGGATCTCGTAT184210.15406752140226285No Hit
CACCCGTAGAACCGACCTTGCATCTCGTAT183340.15333988042935165No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGT173850.14540273924207914No Hit
TTTGTTCGTTCGGCTCGCGTGAAATCTCGT172540.14430709593804047No Hit
CGCGACCTCAGATCAGACGTGGATCTCGTA162640.13602704348767183No Hit
TAGCACCATTTGAAATCGGTTATCTCGTAT159700.1335681188205927No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTA158960.13294920580915098No Hit
TCCAGCATCAGTGATTTTGTTGAATCTCGT158810.1328237504689939No Hit
TCAGTGCACTACAGAACTTTGTCATCTCGT157280.13154410599939145No Hit
AAGCTGCCAGTTGAAGAACTGTAATCTCGT156080.13054046327813468No Hit
TGAGGTAGTAGGTTGTGTGGTTATCTCGTA154590.12929427356590745No Hit
TGAGATGAAGCACTGTAGCATCTCGTATGC152680.12769680890124038No Hit
CTGACCTATGAATTGACAGCCAGATCTCGT150110.12554734073988205No Hit
TAGCACCATCTGAAATCGGTTAATCTCGTA149600.12512079258334793No Hit
TGTAAACATCCTCGACTGGAAGCATCTCGT145670.1218338626712319No Hit
CGCGACCTCAGATCAGACGTGGCGATCTCG143700.12018621587050198No Hit
TGGCTCAGTTCAGCAGGAACAGTATCTCGT140550.11755165372720289No Hit
TTTGGCAATGGTAGAACTCACACTATCTCG138020.11543564032321979No Hit
GTCCAGTTTTCCCAGGAATCCCATCTCGTA131000.1095643304038675No Hit
TGAGATGAAGCACTGTAGAAAATCTCGTAT128140.10717231525153881No Hit
TCGTACCGTGAGTAATAATGCGATCTCGTA128080.10712213311547594No Hit
TACCCTGTAGATCCGAATTTGATCTCGTAT125870.10527375777049468No Hit
ACCCTGTAGAACCGAATTTGTATCTCGTAT124490.10411956864104935No Hit
GTGAAATGTTTAGGACCACTAGAATCTCGT121550.10166064397397019No Hit
AGCAGCATTGTACAGGGCTATGATCTCGTA120410.10070718338877623No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)