FastQCFastQC Report
Thu 9 May 2024
EGAF00001082971

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001082971
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8438302
Total Bases253.1 Mbp
Sequences flagged as poor quality0
Sequence length30
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTATGCCGTCTTCTGCTTGAAAAAA86586510.261128364450574Illumina Paired End PCR Primer 2 (100% over 24bp)
TAGCTTATCAGACTGATGTTGAATCTCGTA7968089.442752819228323No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTA3322903.9378775492984257No Hit
TTTGTTCGTTCGGCTCGCGTGAATCTCGTA3014833.5727922513320807No Hit
TCAGTGCACTACAGAACTTTGATCTCGTAT2942053.486542671736565No Hit
TGACCTATGAATTGACAGCCAGATCTCGTA2689623.1873948099985046No Hit
TGAGATGAAGCACTGTAGCTCTATCTCGTA2467792.924510168040916No Hit
TACCCTGTAGAACCGAATTTGTATCTCGTA2210922.6201005842170617No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTA1983352.35041362586928No Hit
TACCCTGTAGATCCGAATTTGTATCTCGTA1494481.7710672123372688No Hit
TGTAACAGCAACTCCATGTGGAATCTCGTA1356971.6081078871080936No Hit
TGAGATGAAGCACTGTAGCTATCTCGTATG1250011.4813525280322986No Hit
TAGCTTATCAGACTGATGTTGACATCTCGT1088091.2894655820566745No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGT1017221.2054794910160835No Hit
CTGACCTATGAATTGACAGCCATCTCGTAT984761.1670120363077785No Hit
TAGCTTATCAGACTGATGTTGATCTCGTAT801260.9495512248791286No Hit
TCAGTGCACTACAGAACTTTGAATCTCGTA716840.8495074008965311No Hit
TGAGATGAAGCACTGTAGCTCATCTCGTAT596190.7065283987228711No Hit
CTGACCTATGAATTGACAGCCAATCTCGTA580160.6875316858770876No Hit
TAATACTGCCGGGTAATGATGGAATCTCGT531990.6304467415363896No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTA414170.4908214946561524No Hit
TATTGCACTTGTCCCGGCCTGTATCTCGTA390540.4628182304923431No Hit
TACCCTGTAGAACCGAATTTGTGATCTCGT347380.4116704995862912No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAA325340.3855515007640163Illumina Paired End PCR Primer 2 (100% over 23bp)
CAAAGTGCTGTTCGTGCAGGTAGATCTCGT312670.37053663165883377No Hit
GTCTACGGCCATACCACCCTGAACGCGCCC289560.3431496052167841No Hit
CACTAGATTGTGAGCTCCTGGAATCTCGTA267880.3174572325095736No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTA264460.31340428441646195No Hit
TAGCACCATCTGAAATCGGTTATCTCGTAT256140.30354448086830743No Hit
TACAGTACTGTGATAACTGAATCTCGTATG233160.27631151385669767No Hit
TGTAAACATCCTCGACTGGAAGATCTCGTA219860.2605500490501525No Hit
GTGAAATGTTTAGGACCACTAGATCTCGTA216260.2562837878995087No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTA215530.25541868494396147No Hit
TTTGTTCGTTCGGCTCGCGTGATCTCGTAT207530.24593810460919746No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTA202090.23949130998155788No Hit
ACCCTGTAGATCCGAATTTGTATCTCGTAT200340.23741743303332827No Hit
CAACACCAGTCGATGGGCTGTCATCTCGTA189380.22442903797470154No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC184520.2186695854213324No Hit
TAATACTGCCGGGTAATGATGGATCTCGTA173120.20515975844429363No Hit
GCGGCGACTCTGGACGCGAGCATCTCGTAT170960.20260000175390735No Hit
CACCCGTAGAACCGACCTTGCGATCTCGTA170400.20193636113047386No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGT166140.19688795210221202No Hit
TCAGTGCACTACAGAACTTTGTCATCTCGT163280.19349864463253388No Hit
TGTAACAGCAACTCCATGTGGATCTCGTAT150060.17783198562933633No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTAT144790.1715866533338105No Hit
TACAGTACTGTGATAACTGAATATCTCGTA143980.17062674457491567No Hit
TACCCTGTAGAACCGAATTTGATCTCGTAT138680.16434586010313448No Hit
ACCCTGTAGATCCGAATTTGTGAATCTCGT136660.16195201356860658No Hit
TGACCTATGAATTGACAGCCAGTATCTCGT134220.15906043656650354No Hit
GTACAGTACTGTGATAACTGAATCTCGTAT125790.14907027503874595No Hit
TGTAACAGCAACTCCATGTGGAAATCTCGT125690.1489517677845614No Hit
AAGCTGCCAGTTGAAGAACTGTAATCTCGT125020.14815776918152493No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTA124830.14793260539857428No Hit
ACCCTGTAGAACCGAATTTGTGATCTCGTA124560.147612635812276No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTA123150.14594168352827383No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCAT121430.14390335875629956No Hit
TTTGTTCGTTCGGCTCGCGTGAAATCTCGT118800.14078661797124586No Hit
TGAGATGAAGCACTGTAGCTCTTATCTCGT118050.13989781356486175No Hit
TCAGTGCACTACAGAACTTTGTTATCTCGT116850.13847572651464712No Hit
TAGCACCATTTGAAATCGGTTATCTCGTAT113480.1344820320486278No Hit
CGCGACCTCAGATCAGACGTGGATCTCGTA113200.13415021173691105No Hit
CTGACCTATGAATTGACAGCCAGATCTCGT109890.13022762162340243No Hit
TAGCTTATCAGACTGATGTTGAAATCTCGT105760.1253332720255805No Hit
TGAAATGTTTAGGACCACTAGATCTCGTAT105660.12521476477139595No Hit
TGAGATGAAGCACTGTAGCTCAATCTCGTA105550.12508440679179295No Hit
CTGACCTATGAATTGACAGCCATATCTCGT105390.12489479518509768No Hit
CGGCGACTCTGGACGCGAGCATCTCGTATG104400.12372157336867061No Hit
TACCCTGTAGATCCGAATTTGATCTCGTAT103490.1226431573555912No Hit
TCGTTTCCGGCTCGAAGGACCAATCTCGTA102120.12101960797326286No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGC92720.10987992607991513No Hit
TTTGGCAATGGTAGAACTCACAATCTCGTA92100.10914518110397091No Hit
TGAGATGAAGCACTGTAGCTCTAATCTCGT91460.10838673467718979No Hit
ACCCTGTAGAACCGAATTTGTATCTCGTAT91160.10803121291463615No Hit
CTGACCTATGAATTGACAGCATCTCGTATG90310.10702390125406747No Hit
CACCCGTAGAACCGACCTTGCATCTCGTAT89750.10636026063063399No Hit
CATCTTACCGGACAGTGCTGGAATCTCGTA89530.10609954467142796No Hit
ACTGGACTTGGAGTCAGAAGGCATCTCGTA88930.10538850114632067No Hit
TCCAGCATCAGTGATTTTGTTGAATCTCGT85820.10170292554118116No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)