FastQCFastQC Report
Wed 8 May 2024
EGAF00001082972

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001082972
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6029381
Total Bases180.8 Mbp
Sequences flagged as poor quality0
Sequence length30
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCTTATCAGACTGATGTTGAATCTCGTA5991729.937537534947618No Hit
ATCTCGTATGCCGTCTTCTGCTTGAAAAAA5918249.81566764482125Illumina Paired End PCR Primer 2 (100% over 24bp)
TACCCTGTAGATCCGAATTTGTATCTCGTA3703146.141824509016764No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTA2593334.301154629306059No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTA1772622.9399701229695054No Hit
TGACCTATGAATTGACAGCCAGATCTCGTA1619292.6856654107610716No Hit
TAGCTTATCAGACTGATGTTGACATCTCGT1411992.341849022312572No Hit
TACCCTGTAGAACCGAATTTGTATCTCGTA1353582.2449734060594277No Hit
TTTGTTCGTTCGGCTCGCGTGAATCTCGTA1180581.9580451127570144No Hit
TGAGATGAAGCACTGTAGCTATCTCGTATG1044161.7317863973101053No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGT912641.5136545525983514No Hit
TGTAACAGCAACTCCATGTGGAATCTCGTA769391.276067974473665No Hit
TGAGATGAAGCACTGTAGCTCTATCTCGTA768571.2747079675343123No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGT564000.9354194070668281No Hit
CTGACCTATGAATTGACAGCCATCTCGTAT561250.9308584081848534No Hit
TCAGTGCACTACAGAACTTTGATCTCGTAT551860.9152846701842197No Hit
TAGCTTATCAGACTGATGTTGATCTCGTAT531960.882279623729202No Hit
GTGAAATGTTTAGGACCACTAGATCTCGTA516590.856787786341583No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTA486730.8072636312085768No Hit
TAATACTGCCGGGTAATGATGGAATCTCGT475180.7881074359042827No Hit
TATTGCACTTGTCCCGGCCTGTATCTCGTA464840.7709580801080574No Hit
CACCCGTAGAACCGACCTTGCGATCTCGTA432030.7165412170834784No Hit
ACCCTGTAGATCCGAATTTGTATCTCGTAT400280.6638824118097696No Hit
CTGACCTATGAATTGACAGCCAATCTCGTA318340.5279812305774009No Hit
TTTGGCAATGGTAGAACTCACACTATCTCG309070.5126065179825259No Hit
TCAGTGCACTACAGAACTTTGTCATCTCGT294280.4880766367227415No Hit
TGAAATGTTTAGGACCACTAGATCTCGTAT271640.4505271768362291No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTA270710.44898472994159766No Hit
TACCCTGTAGAACCGAATTTGTGATCTCGT264840.4392490705098915No Hit
ACCCTGTAGATCCGAATTTGTGAATCTCGT224770.3727911704368989No Hit
TTTGGCAATGGTAGAACTCACAATCTCGTA216760.3595062246024924No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTA207630.3443637083143361No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAA198640.3294533883328985Illumina Paired End PCR Primer 2 (100% over 23bp)
TGAGATGAAGCACTGTAGCTCATCTCGTAT170910.2834619341521128No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGT170570.2828980288357959No Hit
TACCCTGTAGATCCGAATTTGATCTCGTAT165280.2741243255319244No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC162210.2690325922345926No Hit
TCAGTGCACTACAGAACTTTGAATCTCGTA160550.2662794074549278No Hit
CAACACCAGTCGATGGGCTGTCATCTCGTA159270.26415646979349955No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTA156280.25919742010000696No Hit
GTGAAATGTTTAGGACCACTAGAATCTCGT146030.24219733335810092No Hit
TAGCACCATCTGAAATCGGTTATCTCGTAT126860.21040302478811673No Hit
CACCCGTAGAACCGACCTTGCATCTCGTAT123090.20415030995719127No Hit
TAATACTGCCGGGTAATGATGGATCTCGTA119230.19774832607194667No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTAT118710.19688588264699147No Hit
TACCCTGTAGATCCGAATTTGTGAATCTCG117030.19409952696636687No Hit
CATCTTACCGGACAGTGCTGGAATCTCGTA114950.19064975326654593No Hit
ACCCTGTAGATCCGAATTTGTGTATCTCGT109810.18212483171987306No Hit
CACTAGATTGTGAGCTCCTGGAATCTCGTA107150.17771310189221745No Hit
TCAGTGCACTACAGAACTTTGTTATCTCGT104610.17350039747032073No Hit
TAGCTTATCAGACTGATGTTGACTATCTCG104300.17298624850544358No Hit
TTTGGCAATGGTAGAACTCACACTGATCTC94080.1560359181149773No Hit
TATGGCACTGGTAGAATTCACTGATCTCGT94000.15590323451113802No Hit
TACCCTGTAGAACCGAATTTGATCTCGTAT93460.155007620185223No Hit
ACCCTGTAGAACCGAATTTGTGATCTCGTA92640.1536476132458705No Hit
TTTGGCAATGGTAGAACTCACACATCTCGT90320.14979978873453179No Hit
AAGCTGCCAGTTGAAGAACTGTAATCTCGT87630.14533830255543645No Hit
TCAGTGCACTACAGAACTTTGTCTATCTCG87260.14472464088767983No Hit
TACCCTGTAGATCCGAATTTGTGTATCTCG87150.14454220093240086No Hit
TAGCTTATCAGACTGATGTTGAAATCTCGT81490.13515483596077277No Hit
TGAAATGTTTAGGACCACTAGTATCTCGTA79460.13178798951335136No Hit
TAGCTTATCAGACTGATGTTGACAATCTCG79040.1310914005931952No Hit
TGAGGTAGTAGGTTGTGTGGTTATCTCGTA78270.1298143209062423No Hit
TCGTACCGTGAGTAATAATGCGATCTCGTA77660.12880260842696786No Hit
TCCAGCATCAGTGATTTTGTTGAATCTCGT76090.1261986927016223No Hit
TGTAAACATCCTCGACTGGAAGATCTCGTA74980.12435770769835244No Hit
ACCCTGTAGATCCGAATTTGTGATCTCGTA73270.12152159566628812No Hit
TACCCTGTAGATCCGAATTTGTGATCTCGT72420.12011183237549591No Hit
CTGTGCGTGTGACAGCGGCTGAATCTCGTA71270.11820450557030646No Hit
CATGCCTTGAGTGTAGGACCGTATCTCGTA71130.11797230926358776No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTA70340.11666205867567499No Hit
CAAAGTGCTGTTCGTGCAGGTAGAATCTCG69180.11473814642000563No Hit
TACAGTACTGTGATAACTGAATCTCGTATG68500.11361033578737188No Hit
TACAGTACTGTGATAACTGAATATCTCGTA67730.11233325610041894No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTA65240.10820347893092175No Hit
TGAGATGAAGCACTGTAGATCTCGTATGCC63750.10573224680941543No Hit
TGTAACAGCAACTCCATGTGGAAATCTCGT63090.10463760707774149No Hit
TGTAACAGCAACTCCATGTGGATCTCGTAT62990.10447175257294239No Hit
ACCGGGTGCTGTAGGCTTTATCTCGTATGC62070.10294589112879082No Hit
CTGACCTATGAATTGACAGCCATATCTCGT61700.10233222946103424No Hit
TAGCACCATCTGAAATCGGTTAATCTCGTA61020.10120441882840046No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)