FastQCFastQC Report
Wed 8 May 2024
EGAF00001082973

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001082973
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12484613
Total Bases374.5 Mbp
Sequences flagged as poor quality0
Sequence length30
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCCTGTAGAACCGAATTTGTATCTCGTA10787498.640628267772497No Hit
TGAGATGAAGCACTGTAGCTCTATCTCGTA10037998.040289274485321No Hit
ATCTCGTATGCCGTCTTCTGCTTGAAAAAA8819457.064255816339681Illumina Paired End PCR Primer 2 (100% over 24bp)
TAGCTTATCAGACTGATGTTGAATCTCGTA6828515.469540785925843No Hit
TGACCTATGAATTGACAGCCAGATCTCGTA5836974.675331145627021No Hit
TACCCTGTAGATCCGAATTTGTATCTCGTA5118534.099870776931572No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTA4489643.5961387028977194No Hit
TTTGTTCGTTCGGCTCGCGTGAATCTCGTA3333412.6700146812720584No Hit
TCAGTGCACTACAGAACTTTGATCTCGTAT2958672.3698531944882872No Hit
TGAGATGAAGCACTGTAGCTATCTCGTATG2944182.358246907613396No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTA2258371.8089227115009492No Hit
TGTAACAGCAACTCCATGTGGAATCTCGTA1982311.5878025213917326No Hit
TACCCTGTAGAACCGAATTTGTGATCTCGT1980311.5862005494283242No Hit
TGAGATGAAGCACTGTAGCTCATCTCGTAT1788441.4325153691187704No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGT1472171.1791875326852341No Hit
TCAGTGCACTACAGAACTTTGAATCTCGTA1412131.1310963343437237No Hit
CTGACCTATGAATTGACAGCCATCTCGTAT1035260.8292287474189228No Hit
TAGCTTATCAGACTGATGTTGACATCTCGT907750.7270950248918409No Hit
TAATACTGCCGGGTAATGATGGAATCTCGT884240.708263844461979No Hit
TGTAAACATCCTCGACTGGAAGATCTCGTA816450.6539650047622622No Hit
ACCCTGTAGATCCGAATTTGTATCTCGTAT601380.4816969496771746No Hit
CTGACCTATGAATTGACAGCCAATCTCGTA597310.4784369367316392No Hit
ACCCTGTAGAACCGAATTTGTGATCTCGTA596600.4778682366846293No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTA561490.44974561886700054No Hit
TGAGATGAAGCACTGTAGCTCTTATCTCGT527470.42249607576942916No Hit
TACCCTGTAGAACCGAATTTGATCTCGTAT478380.3831756739275779No Hit
TGAGATGAAGCACTGTAGCTCAATCTCGTA475500.3808688343002703No Hit
TACAGTACTGTGATAACTGAATCTCGTATG463080.37092058840750614No Hit
TAGCACCATCTGAAATCGGTTATCTCGTAT460430.3687979755559904No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTA436770.3498466472288729No Hit
ACCCTGTAGATCCGAATTTGTGAATCTCGT426860.34190887615018584No Hit
GTGAAATGTTTAGGACCACTAGATCTCGTA414050.33164824572455714No Hit
TGAGATGAAGCACTGTAGCTCTAATCTCGT395290.31662174870778936No Hit
ACCCTGTAGAACCGAATTTGTATCTCGTAT365600.29284047491099646No Hit
TACCCTGTAGAACCGAATTTGTGAATCTCG358930.2874978984130305No Hit
ACCCTGTAGAACCGAATTTGTGTATCTCGT318470.25509000559328515No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGT309160.2476328261036205No Hit
CACTAGATTGTGAGCTCCTGGAATCTCGTA308080.24676776124338012No Hit
TAGCTTATCAGACTGATGTTGATCTCGTAT307600.2463832879721622No Hit
TAATACTGCCGGGTAATGATGGATCTCGTA300260.24050405086645457No Hit
ACCCTGTAGAACCGAATTTGTGAATCTCGT280810.22492487352231105No Hit
TACAGTACTGTGATAACTGAATATCTCGTA280690.22482875520450654No Hit
CAACACCAGTCGATGGGCTGTCATCTCGTA275810.2209199436137908No Hit
TTTGGCAATGGTAGAACTCACACTATCTCG255330.2045157507084921No Hit
TGAGATGAAGCACTGTAGATCTCGTATGCC253400.20296984776380333No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTA251310.20129578706204188No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTA235260.18843996205569208No Hit
TACCCTGTAGATCCGAATTTGTGAATCTCG231770.18564452097954498No Hit
TATTGCACTTGTCCCGGCCTGTATCTCGTA228680.1831694742960795No Hit
GTCTACGGCCATACCACCCTGAACGCGCCC228470.18300126723992166No Hit
TGTAACAGCAACTCCATGTGGATCTCGTAT221430.17736232592872522No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTA217520.17423047074026243No Hit
TGTAACAGCAACTCCATGTGGAAATCTCGT215410.17254039031886692No Hit
TGAGATGAAGCACTGTAGCTAATCTCGTAT212550.17024957041119335No Hit
TACCCTGTAGATCCGAATTTGATCTCGTAT211520.1694245548500382No Hit
TCCAGCATCAGTGATTTTGTTGAATCTCGT209940.16815899699894582No Hit
TGACCTATGAATTGACAGCCAGTATCTCGT209810.16805486882132428No Hit
AAGCTGCCAGTTGAAGAACTGTAATCTCGT209230.16759029695193595No Hit
TGAGATGAAGCACTGTAGAAAATCTCGTAT208870.1673019419985225No Hit
TTTGGCAATGGTAGAACTCACAATCTCGTA208770.1672218434003521No Hit
TACCCTGTAGAACCGAATTTGTGTATCTCG208570.1670616462040113No Hit
AACCCGTAGATCCGAACTTGTGAATCTCGT196350.15727359750758793No Hit
GTACAGTACTGTGATAACTGAATCTCGTAT193670.1551269550766211No Hit
CTGACCTATGAATTGACAGCCAGATCTCGT193500.15499078745973144No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAA188980.15137033082242918Illumina Paired End PCR Primer 2 (100% over 23bp)
CTTTCAGTCGGATGTTTACAGATCTCGTAT181990.14577143881031795No Hit
ATGACCTATGAATTGACAGACAATCTCGTA173460.1389390283863825No Hit
GTGAAATGTTTAGGACCACTAGAATCTCGT169030.13539066048743362No Hit
TTTGTTCGTTCGGCTCGCGTGATCTCGTAT167380.13406903361762196No Hit
TGAGATGAAGCACTGTAGAATCTCGTATGC166960.13373261950530624No Hit
TACCCTGTAGAACCGAATTTGCATCTCGTA162570.13021629104562552No Hit
TGAAATGTTTAGGACCACTAGATCTCGTAT162120.12985584735385872No Hit
CACCCGTAGAACCGACCTTGCGATCTCGTA161100.12903884165252058No Hit
TCAGTGCACTACAGAACTTTGTCATCTCGT160330.12842208244660847No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGT159590.12782935282014748No Hit
TGTAAACATCCTCGACTGGAAGCATCTCGT157550.12619534141747124No Hit
CATCTTACCGGACAGTGCTGGAATCTCGTA154200.12351203837876272No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTA147580.118209511179882No Hit
ACCCTGTAGATCCGAATTTGTGTATCTCGT141920.11367593052343714No Hit
CACCCGTAGAACCGACCTTGCATCTCGTAT133210.10669934262279497No Hit
CTGACCTATGAATTGACAGCATCTCGTATG130500.10452867061237703No Hit
TAGCACCATTTGAAATCGGTTATCTCGTAT125960.10089219425544067No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)