FastQCFastQC Report
Wed 8 May 2024
EGAF00001082974

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001082974
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8112826
Total Bases243.3 Mbp
Sequences flagged as poor quality0
Sequence length30
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTATGCCGTCTTCTGCTTGAAAAAA84887510.463369977366703Illumina Paired End PCR Primer 2 (100% over 24bp)
TTTGTTCGTTCGGCTCGCGTGAATCTCGTA4952226.104186136865255No Hit
TGACCTATGAATTGACAGCCAGATCTCGTA4706555.8013693378854665No Hit
TACCCTGTAGAACCGAATTTGTATCTCGTA3711594.5749656161736985No Hit
TAGCTTATCAGACTGATGTTGAATCTCGTA2863123.5291278279603184No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTA2846053.5080870710156984No Hit
TGAGATGAAGCACTGTAGCTCTATCTCGTA2803093.4551338830636826No Hit
TCAGTGCACTACAGAACTTTGATCTCGTAT2515383.1004979029502175No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTA2062932.5428007453876122No Hit
CTGACCTATGAATTGACAGCCATCTCGTAT1883132.321176369368701No Hit
TACCCTGTAGATCCGAATTTGTATCTCGTA1799752.218400838376171No Hit
TGTAACAGCAACTCCATGTGGAATCTCGTA1381341.7026619330921187No Hit
CTGACCTATGAATTGACAGCCAATCTCGTA1285701.5847745286291115No Hit
TGAGATGAAGCACTGTAGCTATCTCGTATG1005631.2395557355722902No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGT803410.9902961064368939No Hit
TAATACTGCCGGGTAATGATGGAATCTCGT705670.8698202081494167No Hit
TACCCTGTAGAACCGAATTTGTGATCTCGT697130.8592936665965719No Hit
TCAGTGCACTACAGAACTTTGAATCTCGTA668950.8245585447043976No Hit
TGAGATGAAGCACTGTAGCTCATCTCGTAT601930.7419486132205966No Hit
TAGCTTATCAGACTGATGTTGACATCTCGT471730.581461996103454No Hit
GTCTACGGCCATACCACCCTGAACGCGCCC427480.5269187333735494No Hit
TATTGCACTTGTCCCGGCCTGTATCTCGTA398600.4913207802065519No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTA389680.4803258445330887No Hit
GTGAAATGTTTAGGACCACTAGATCTCGTA358400.44176961270955395No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAA334490.41229776159380216Illumina Paired End PCR Primer 2 (100% over 23bp)
CAAAGTGCTGTTCGTGCAGGTAGATCTCGT297750.36701144582664535No Hit
TAGCACCATCTGAAATCGGTTATCTCGTAT295770.36457086593500215No Hit
CGCGACCTCAGATCAGACGTGGATCTCGTA279070.3439861769499309No Hit
TAGCTTATCAGACTGATGTTGATCTCGTAT256070.31563600649144946No Hit
CTGACCTATGAATTGACAGCCATATCTCGT253690.3127023801570501No Hit
TTTGTTCGTTCGGCTCGCGTGATCTCGTAT249610.3076733064409368No Hit
TGTAAACATCCTCGACTGGAAGATCTCGTA247880.3055408805760163No Hit
CACCCGTAGAACCGACCTTGCGATCTCGTA247660.3052697050325004No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTA245960.30317425765078654No Hit
ACCCTGTAGATCCGAATTTGTATCTCGTAT231810.28573273973828606No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTA229060.28234304544433714No Hit
TAATACTGCCGGGTAATGATGGATCTCGTA216800.26723117197385965No Hit
TACCCTGTAGAACCGAATTTGATCTCGTAT211300.2604517833859619No Hit
CACTAGATTGTGAGCTCCTGGAATCTCGTA210700.2597122137218277No Hit
TCAGTGCACTACAGAACTTTGTCATCTCGT203950.2513920550003168No Hit
ACCCTGTAGAACCGAATTTGTGATCTCGTA199700.24615343654603217No Hit
TGACCTATGAATTGACAGCCAGTATCTCGT198320.24445242631852326No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGT183660.2263822741915086No Hit
CTGACCTATGAATTGACAGCCAGATCTCGT175630.2164843668531779No Hit
CTGACCTATGAATTGACAGCATCTCGTATG175250.2160159727325595No Hit
TACAGTACTGTGATAACTGAATCTCGTATG171780.2117387948416495No Hit
TGTAAACATCCTTGACTGGAAGCATCTCGT171070.21086363740575723No Hit
TGAAATGTTTAGGACCACTAGATCTCGTAT164390.20262976181172873No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTA163360.20136016722163155No Hit
TTTGTTCGTTCGGCTCGCGTGAAATCTCGT159020.1960106133177268No Hit
TGTAACAGCAACTCCATGTGGATCTCGTAT158840.19578874241848648No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTA158820.19576409009634865No Hit
TGTAACAGCAACTCCATGTGGAAATCTCGT158040.19480264953297408No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTA157370.19397679674135745No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC153760.18952705259548275No Hit
ACCCTGTAGATCCGAATTTGTGAATCTCGT151200.18637155536184308No Hit
CACCCGTAGAACCGACCTTGCATCTCGTAT144840.17853211692201953No Hit
CGCGACCTCAGATCAGACGTGGCGATCTCG142080.17513009646700176No Hit
TAACACTGTCTGGTAAAGATGGCATCTCGT140170.1727757997028409No Hit
TCGTACCGTGAGTAATAATGCGATCTCGTA138070.17018730587837086No Hit
TTTGGCAATGGTAGAACTCACACTATCTCG135910.1675248550874874No Hit
ACCCTGTAGAACCGAATTTGTATCTCGTAT130980.16144805768051723No Hit
GTGAAATGTTTAGGACCACTAGAATCTCGT130540.16090570659348544No Hit
ACTGGACTTGGAGTCAGAAGGCATCTCGTA128850.15882258537284047No Hit
TGAGATGAAGCACTGTAGCTCTTATCTCGT123800.15259787403304348No Hit
TTTGGCAATGGTAGAACTCACAATCTCGTA119950.1478523020215151No Hit
TACCCTGTAGAACCGAATTTGTGAATCTCG117530.14486937104284006No Hit
TCAGTGCACTACAGAACTTTGTTATCTCGT116650.14378466886877644No Hit
TACAGTACTGTGATAACTGAATATCTCGTA114130.14067847627941238No Hit
TGAGATGAAGCACTGTAGCTCAATCTCGTA113170.1394951648167975No Hit
TGGCTCAGTTCAGCAGGAACAGTATCTCGT108010.13313486570524255No Hit
AAGCTGCCAGTTGAAGAACTGTAATCTCGT105210.12968354060594914No Hit
ACCCTGTAGAACCGAATTTGTGTATCTCGT101910.1256159074532105No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTAT101080.1245928360844914No Hit
TGAGATGAAGCACTGTAGCTCTAATCTCGT100470.1238409402592882No Hit
GTCCAGTTTTCCCAGGAATCCCTATCTCGT95980.11830649393934987No Hit
TTCAAGTAATCCAGGATAGGCTATCTCGTA95160.11729574873169965No Hit
TTGTTCGTTCGGCTCGCGTGAATCTCGTAT94800.11685200693321908No Hit
TAGCACCATCTGAAATCGGTTAATCTCGTA92450.11395535908202642No Hit
GTACAGTACTGTGATAACTGAATCTCGTAT92120.11354859576675255No Hit
ACCCTGTAGAACCGAATTTGTGAATCTCGT91720.11305554932399635No Hit
TAGCACCATTTGAAATCGGTTATCTCGTAT91370.11262413368658467No Hit
TACCCTGTAGATCCGAATTTGATCTCGTAT91130.11232830582093097No Hit
TGAGGTAGTAGGTTGTGTGGTTATCTCGTA88360.10891395920484429No Hit
TGAGATGAAGCACTGTAGATCTCGTATGCC85140.10494493534065688No Hit
TGTAAACATCCTTGACTGGAAGATCTCGTA84500.10415606103224696No Hit
TAATACTGCCTGGTAATGATGAATCTCGTA83660.10312066350245895No Hit
TTTTGTTCGTTCGGCTCGCGTGAATCTCGT83650.10310833734139004No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)