FastQCFastQC Report
Wed 8 May 2024
EGAF00001082975

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001082975
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6722757
Total Bases201.6 Mbp
Sequences flagged as poor quality0
Sequence length30
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTATGCCGTCTTCTGCTTGAAAAAA6363189.465134616646118Illumina Paired End PCR Primer 2 (100% over 24bp)
TGAGATGAAGCACTGTAGCTCTATCTCGTA3563485.300622943830931No Hit
TAGCTTATCAGACTGATGTTGAATCTCGTA3525275.243786143095757No Hit
TACCCTGTAGAACCGAATTTGTATCTCGTA3324554.945218159752018No Hit
TACCCTGTAGATCCGAATTTGTATCTCGTA2902934.318064746353319No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTA2704434.02279897964481No Hit
TTTGTTCGTTCGGCTCGCGTGAATCTCGTA2264173.367918846389956No Hit
TGACCTATGAATTGACAGCCAGATCTCGTA2188993.256089726283428No Hit
TCAGTGCACTACAGAACTTTGATCTCGTAT1503502.236433653633472No Hit
TGAGATGAAGCACTGTAGCTATCTCGTATG1265791.8828436012189642No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTA1082481.610172731217267No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGT1020571.5180825366735702No Hit
TGTAACAGCAACTCCATGTGGAATCTCGTA978141.4549685493615194No Hit
TGAGATGAAGCACTGTAGCTCATCTCGTAT688001.0233896599267236No Hit
TCAGTGCACTACAGAACTTTGAATCTCGTA599200.8913009945175767No Hit
CTGACCTATGAATTGACAGCCATCTCGTAT575860.8565830952985508No Hit
TAGCTTATCAGACTGATGTTGACATCTCGT524790.7806172378385832No Hit
TAATACTGCCGGGTAATGATGGAATCTCGT467750.6957710951027979No Hit
TACCCTGTAGAACCGAATTTGTGATCTCGT466930.6945513574267224No Hit
TATTGCACTTGTCCCGGCCTGTATCTCGTA428420.6372683112003007No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGT417330.6207721028738655No Hit
TGTAAACATCCTCGACTGGAAGATCTCGTA411170.6116091954535915No Hit
CTGACCTATGAATTGACAGCCAATCTCGTA357530.5318205016186067No Hit
ACCCTGTAGATCCGAATTTGTATCTCGTAT343030.5102519695416627No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTA323220.4807848922696447No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTA320330.47648606070396415No Hit
GTGAAATGTTTAGGACCACTAGATCTCGTA268130.3988393452269656No Hit
TAGCTTATCAGACTGATGTTGATCTCGTAT264120.39287453049396254No Hit
CACTAGATTGTGAGCTCCTGGAATCTCGTA254290.3782525532307653No Hit
ACCCTGTAGATCCGAATTTGTGAATCTCGT235840.35080845551906753No Hit
TACAGTACTGTGATAACTGAATCTCGTATG230130.3423149163356641No Hit
TAGCACCATCTGAAATCGGTTATCTCGTAT203140.3021676969731317No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAA200510.2982556114998653Illumina Paired End PCR Primer 2 (100% over 23bp)
CTTTCAGTCGGATGTTTACAGTATCTCGTA197790.2942096523792248No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTA185630.2761218351340083No Hit
TACCCTGTAGAACCGAATTTGATCTCGTAT183010.2722246245104501No Hit
CACCCGTAGAACCGACCTTGCGATCTCGTA179830.2674944223032306No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTA176220.262124601558557No Hit
TACAGTACTGTGATAACTGAATATCTCGTA176100.26194610336205815No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGT172780.2570076532589234No Hit
TGAGATGAAGCACTGTAGCTCTTATCTCGT157730.23462100444802633No Hit
ACCCTGTAGAACCGAATTTGTGATCTCGTA155030.23060479502680226No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTA142310.21168398619792447No Hit
TGTAACAGCAACTCCATGTGGAAATCTCGT139830.20799502347028162No Hit
TGAGATGAAGCACTGTAGCTCAATCTCGTA137460.2044696840894294No Hit
AACCCGTAGATCCGAACTTGTGAATCTCGT135770.20195583448873727No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCAT134630.20026010162199825No Hit
TACCCTGTAGATCCGAATTTGTGAATCTCG128690.1914244408953053No Hit
GTCTACGGCCATACCACCCTGAACGCGCCC126870.18871721824840612No Hit
ACCCTGTAGAACCGAATTTGTATCTCGTAT124340.1849538812722221No Hit
TGAAATGTTTAGGACCACTAGATCTCGTAT122880.18278215321481944No Hit
TGAGATGAAGCACTGTAGCTCTAATCTCGT122320.18194916163115818No Hit
TAATACTGCCGGGTAATGATGGATCTCGTA117330.17452661162674776No Hit
TACCCTGTAGATCCGAATTTGATCTCGTAT109350.16265648155957443No Hit
TCGTTTCCGGCTCGAAGGACCAATCTCGTA109110.16229948516657675No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTAT109040.16219536121861908No Hit
TTTGTTCGTTCGGCTCGCGTGATCTCGTAT109010.1621507366694944No Hit
AAGCTGCCAGTTGAAGAACTGTAATCTCGT105410.15679579077452896No Hit
GTACAGTACTGTGATAACTGAATCTCGTAT104780.15585867524291003No Hit
TTTGGCAATGGTAGAACTCACACTATCTCG103300.1536571974860909No Hit
CACCCGTAGAACCGACCTTGCATCTCGTAT99550.14807912884550192No Hit
TGAGATGAAGCACTGTAGCTAATCTCGTAT98660.1467552672214688No Hit
CATCTTACCGGACAGTGCTGGAATCTCGTA96600.14369104818157194No Hit
CGGCGACTCTGGACGCGAGCCGGGATCTCG95010.14132594707796223No Hit
GTGAAATGTTTAGGACCACTAGAATCTCGT93780.13949634056384902No Hit
TCAGTGCACTACAGAACTTTGTCATCTCGT93750.1394517160147243No Hit
TGAGATGAAGCACTGTAGATCTCGTATGCC93180.1386038495813548No Hit
CAACACCAGTCGATGGGCTGTCATCTCGTA90360.13440914196363188No Hit
CTGACCTATGAATTGACAGCCAGATCTCGT89440.13304065579047405No Hit
CGCGACCTCAGATCAGACGTGGATCTCGTA88970.1323415378541869No Hit
ACTGGACTTGGAGTCAGAAGGCATCTCGTA88740.13199941631089745No Hit
TAGCACCATTTGAAATCGGTTATCTCGTAT88030.1309433019816126No Hit
GCGGCGGCGGCGACTCTGGACGCGAGCATC87920.130779678634822No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC86850.1291880697160406No Hit
ACCCTGTAGAACCGAATTTGTGAATCTCGT85950.12784933324229925No Hit
TACCCTGTAGAACCGAATTTGTGAATCTCG85540.12723946440426154No Hit
TGTAACAGCAACTCCATGTGGATCTCGTAT81860.12176551971163022No Hit
ACCCTGTAGATCCGAATTTGTGTATCTCGT77860.11581557982833532No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCATC75810.11276623563814667No Hit
ACCCTGTAGAACCGAATTTGTGTATCTCGT75400.11215636680010894No Hit
TTTGTTCGTTCGGCTCGCGTGAAATCTCGT74570.11092175427432525No Hit
TGAGATGAAGCACTGTAGAAAATCTCGTAT74150.11029701058657929No Hit
CAAAGTGCTGTTCGTGCAGGTAGAATCTCG71180.10587918022323282No Hit
CTTTCAGTCGGATGTTTACAGATCTCGTAT71020.10564118262790102No Hit
TGACCTATGAATTGACAGCCAGTATCTCGT69750.10375207671495489No Hit
TTTGGCAATGGTAGAACTCACAATCTCGTA69410.10324633182487483No Hit
GCATGTGGTTCAGTGGTAGAATTCTCATCT68640.10210096839734055No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)