FastQCFastQC Report
Wed 8 May 2024
EGAF00001082976

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001082976
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6760950
Total Bases202.8 Mbp
Sequences flagged as poor quality0
Sequence length30
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTATGCCGTCTTCTGCTTGAAAAAA76592611.328674224776105Illumina Paired End PCR Primer 2 (100% over 24bp)
TTTGTTCGTTCGGCTCGCGTGAATCTCGTA4684056.928094424600093No Hit
TAGCTTATCAGACTGATGTTGAATCTCGTA3361344.971697764367434No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTA2990284.422869567146629No Hit
TGACCTATGAATTGACAGCCAGATCTCGTA2628903.8883588844762937No Hit
TCAGTGCACTACAGAACTTTGATCTCGTAT2109693.120404676857542No Hit
TACCCTGTAGAACCGAATTTGTATCTCGTA2009062.971564646980084No Hit
TGAGATGAAGCACTGTAGCTCTATCTCGTA1716012.5381196429495856No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTA1630082.411022119672531No Hit
TACCCTGTAGATCCGAATTTGTATCTCGTA1245751.8425665032280967No Hit
TGTAACAGCAACTCCATGTGGAATCTCGTA1048921.551438777094935No Hit
CTGACCTATGAATTGACAGCCATCTCGTAT1044161.5443983463862327No Hit
TGAGATGAAGCACTGTAGCTATCTCGTATG694931.0278585110080685No Hit
CTGACCTATGAATTGACAGCCAATCTCGTA643660.9520259726813539No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGT628230.9292037361613383No Hit
TCAGTGCACTACAGAACTTTGAATCTCGTA526540.7787958792773205No Hit
TAATACTGCCGGGTAATGATGGAATCTCGT451930.6684415651646588No Hit
TAGCTTATCAGACTGATGTTGACATCTCGT451440.6677168149446453No Hit
TGAGATGAAGCACTGTAGCTCATCTCGTAT415340.6143219517967149No Hit
CGCGACCTCAGATCAGACGTGGATCTCGTA357450.5286978900894105No Hit
TACCCTGTAGAACCGAATTTGTGATCTCGT330610.4889993270176528No Hit
TAGCTTATCAGACTGATGTTGATCTCGTAT329660.4875941990400757No Hit
GTGAAATGTTTAGGACCACTAGATCTCGTA311080.4601128539628307No Hit
TTTGTTCGTTCGGCTCGCGTGATCTCGTAT299110.44240824144535895No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTA296830.4390359342991739No Hit
TAGCACCATCTGAAATCGGTTATCTCGTAT278590.4120574771296934No Hit
TATTGCACTTGTCCCGGCCTGTATCTCGTA273800.4049726739585413No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAA264930.39185321589421607Illumina Paired End PCR Primer 2 (100% over 23bp)
GTCTACGGCCATACCACCCTGAACGCGCCC242440.3585886598776799No Hit
CGGCGACTCTGGACGCGAGCATCTCGTATG231310.3421264763088028No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTA223820.33104815151716843No Hit
GCGGCGACTCTGGACGCGAGCATCTCGTAT222340.32885911003631146No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC196240.2902550677049823No Hit
ACCCTGTAGATCCGAATTTGTATCTCGTAT177160.26203418158690717No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGT171060.2530117808887804No Hit
TAATACTGCCGGGTAATGATGGATCTCGTA161060.2382209600721792No Hit
TGTAAACATCCTCGACTGGAAGATCTCGTA160050.23672708716970248No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTA159320.23564735725009062No Hit
TTTGTTCGTTCGGCTCGCGTGAAATCTCGT159160.23541070411702497No Hit
GCGGCGGCGGCGACTCTGGACGCGAGCATC156300.23118052936347702No Hit
CACTAGATTGTGAGCTCCTGGAATCTCGTA153020.22632914013563182No Hit
TGTAACAGCAACTCCATGTGGATCTCGTAT146390.2165228259342252No Hit
CACCCGTAGAACCGACCTTGCGATCTCGTA144180.21325405453375634No Hit
TGACCTATGAATTGACAGCCAGTATCTCGT142400.21062128842840133No Hit
TACAGTACTGTGATAACTGAATCTCGTATG137450.20329983212418373No Hit
TACCCTGTAGAACCGAATTTGATCTCGTAT137300.2030779698119347No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTA136590.20202782153395604No Hit
CTGACCTATGAATTGACAGCCATATCTCGT135280.20009022400698126No Hit
GTGAAATGTTTAGGACCACTAGAATCTCGT134960.19961691774085No Hit
CGCGACCTCAGATCAGACGTGGCGATCTCG134450.19886258587920336No Hit
TGAAATGTTTAGGACCACTAGATCTCGTAT134170.1984484428963385No Hit
TCAGTGCACTACAGAACTTTGTCATCTCGT130620.1931977015064451No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTA127150.18806528668308448No Hit
CTGACCTATGAATTGACAGCATCTCGTATG121500.17970847292170478No Hit
TTTGGCAATGGTAGAACTCACAATCTCGTA117780.17420628757792914No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTATC116140.17178059296400655No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCAT115140.1703015108823464No Hit
ACCCTGTAGAACCGAATTTGTGATCTCGTA110550.16351252412752645No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTAT105640.15625023110657527No Hit
AAGCTGCCAGTTGAAGAACTGTAATCTCGT105370.15585087894452704No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGT104850.15508175626206377No Hit
TGTAACAGCAACTCCATGTGGAAATCTCGT104760.15494863887471436No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGA104750.15493384805389776No Hit
CAACACCAGTCGATGGGCTGTCATCTCGTA103490.153070204631006No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTA103200.15264127082732457No Hit
TACAGTACTGTGATAACTGAATATCTCGTA103060.15243419933589214No Hit
CTGACCTATGAATTGACAGCCAGATCTCGT100990.14937249942685568No Hit
ACCCTGTAGATCCGAATTTGTGAATCTCGT100460.14858858592357582No Hit
TTTGGCAATGGTAGAACTCACACTATCTCG99050.14650308018843505No Hit
CGGCGACTCTGGACGCGAGCCGGGATCTCG94580.1398915832834143No Hit
TTGTTCGTTCGGCTCGCGTGAATCTCGTAT94130.13922599634666727No Hit
TAGCACCATTTGAAATCGGTTATCTCGTAT91210.13490707666821972No Hit
TCAGTGCACTACAGAACTTTGTTATCTCGT88660.13113541735998638No Hit
GGCGGCGGCGACTCTGGACGCGAGCATCTC85940.12711231409787085No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTG85920.12708273245623766No Hit
TACCCTGTAGATCCGAATTTGATCTCGTAT84890.12555927791212773No Hit
GCGGCGGCGACTCTGGACGCGAGCATCTCG84340.12474578276721468No Hit
TTTGTTCGTTCGGCTCGCGTGATATCTCGT81190.12008667420998528No Hit
TTTGTTCGTTCGGCTCGCGTGTATCTCGTA80480.11903652593200659No Hit
CGCGACCTCAGATCAGACGTGGCATCTCGT80390.1189034085446572No Hit
CACCCGTAGAACCGACCTTGCATCTCGTAT80050.11840052063689276No Hit
TGAGATGAAGCACTGTAGCTCTTATCTCGT78040.1154275656527559No Hit
TGTAAACATCCTTGACTGGAAGCATCTCGT76400.1130018710388333No Hit
ACCCTGTAGAACCGAATTTGTATCTCGTAT76120.11258772805596846No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGC75910.11227712081881985No Hit
GTACAGTACTGTGATAACTGAATCTCGTAT74870.11073887545389333No Hit
TAGCACCATCTGAAATCGGTTAATCTCGTA74130.10964435471346483No Hit
TGAGGTAGTAGGTTGTGTGGTTATCTCGTA73430.10860899725630274No Hit
ACTGGACTTGGAGTCAGAAGGCATCTCGTA72890.10781029293220627No Hit
GCGGCGGCGGCGACTCTGGACGCGAGATCT71630.10594664950931451No Hit
TTTTGTTCGTTCGGCTCGCGTGAATCTCGT70590.10440840414438798No Hit
GGCGGCGACTCTGGACGCGAGCATCTCGTA70050.10360969982029153No Hit
CGGCGGCGGCGACTCTGGACGCGAGCATCT69580.10291453124191127No Hit
TAATACTGCCTGGTAATGATGAATCTCGTA68960.10199750035128201No Hit
TGTAAACATCCTTGACTGGAAGATCTCGTA68420.10119879602718553No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)