FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001082977

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001082977
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12490203
Sequences flagged as poor quality0
Sequence length30
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGATGAAGCACTGTAGCTCTATCTCGTA146486511.728112025080778No Hit
ATCTCGTATGCCGTCTTCTGCTTGAAAAAA9485847.594624362790581Illumina Single End Adapter 1 (95% over 24bp)
TACCCTGTAGAACCGAATTTGTATCTCGTA9021737.22304513385411No Hit
TAGCTTATCAGACTGATGTTGAATCTCGTA4709603.770635273101646No Hit
TGAGATGAAGCACTGTAGCTATCTCGTATG4522413.620765811412353No Hit
TGACCTATGAATTGACAGCCAGATCTCGTA4188603.353508345701026No Hit
TTTGTTCGTTCGGCTCGCGTGAATCTCGTA3975813.1831428200166165No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTA3569022.8574555593692113No Hit
TACCCTGTAGATCCGAATTTGTATCTCGTA3295212.6382357436464403No Hit
TGAGATGAAGCACTGTAGCTCATCTCGTAT2674812.141526442764781No Hit
TCAGTGCACTACAGAACTTTGATCTCGTAT2315481.8538369632583236No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGT1703981.3642532471249667No Hit
TGTAACAGCAACTCCATGTGGAATCTCGTA1699521.3606824484758175No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTA1683271.3476722516039172No Hit
TACCCTGTAGAACCGAATTTGTGATCTCGT1550291.2412048066792831No Hit
TAATACTGCCGGGTAATGATGGAATCTCGT1107050.8863346736638307No Hit
TGTAAACATCCTCGACTGGAAGATCTCGTA1062770.8508828879722772No Hit
TCAGTGCACTACAGAACTTTGAATCTCGTA1033200.8272083328029176No Hit
TAGCACCATCTGAAATCGGTTATCTCGTAT873310.6991960018584166No Hit
CTGACCTATGAATTGACAGCCATCTCGTAT729560.5841057987608368No Hit
TGAGATGAAGCACTGTAGCTCTTATCTCGT721300.5774926156124124No Hit
TGAGATGAAGCACTGTAGCTCAATCTCGTA704490.5640340673406189No Hit
TGAGATGAAGCACTGTAGCTCTAATCTCGT535170.4284718190729166No Hit
TAGCTTATCAGACTGATGTTGACATCTCGT503710.4032840779289176No Hit
ACCCTGTAGAACCGAATTTGTGATCTCGTA479430.383844842233549No Hit
TACAGTACTGTGATAACTGAATCTCGTATG461840.3697618045119042No Hit
CACTAGATTGTGAGCTCCTGGAATCTCGTA460230.36847279423721135No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTA459110.367576091437425No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTA450080.36034642511414744No Hit
TACCCTGTAGAACCGAATTTGATCTCGTAT441270.353292896840828No Hit
TGAGATGAAGCACTGTAGAAAATCTCGTAT414130.3315638664960049No Hit
TACAGTACTGTGATAACTGAATATCTCGTA412690.3304109628962796No Hit
CGCGACCTCAGATCAGACGTGGATCTCGTA412200.33001865542137304No Hit
ACCCTGTAGATCCGAATTTGTATCTCGTAT398650.3191701527989577No Hit
CTGACCTATGAATTGACAGCCAATCTCGTA395480.3166321636245624No Hit
TAGCACCATTTGAAATCGGTTATCTCGTAT390910.3129732959504341No Hit
TGTAACAGCAACTCCATGTGGAAATCTCGT388340.31091568327592434No Hit
TAATACTGCCGGGTAATGATGGATCTCGTA371950.29779339855405074No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGT368270.2948470893547527No Hit
TATTGCACTTGTCCCGGCCTGTATCTCGTA363740.2912202467806168No Hit
TGAGATGAAGCACTGTAGCTAATCTCGTAT342000.2738146049347637No Hit
TGAGATGAAGCACTGTAGATCTCGTATGCC338030.27063611376052094No Hit
ACCCTGTAGAACCGAATTTGTATCTCGTAT318730.2551840030142024No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTA317370.25409514961446183No Hit
TACCCTGTAGAACCGAATTTGTGAATCTCG306750.24559248556648758No Hit
AACCCGTAGATCCGAACTTGTGAATCTCGT303010.24259813871720098No Hit
ACCCTGTAGATCCGAATTTGTGAATCTCGT300330.2404524570177122No Hit
GTGAAATGTTTAGGACCACTAGATCTCGTA290790.23281447066953193No Hit
TAGCACCATTTGAAATCGGTATCTCGTATG286000.22897946494544563No Hit
ACCCTGTAGAACCGAATTTGTGAATCTCGT281110.2250643964713784No Hit
TGAGATGAAGCACTGTAGAATCTCGTATGC280320.22443190074652908No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTA268500.21496848369878377No Hit
CGGCGGCGGCGGCGACTCTGGACGCGATCT264030.21138967877463638No Hit
ACCCTGTAGAACCGAATTTGTGTATCTCGT256960.205729242350985No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTA255880.204864564651191No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTA244650.19587351782833312No Hit
TAGCTTATCAGACTGATGTTGATCTCGTAT233910.18727477848038177No Hit
TAGCACCATCTGAAATCGGTTAATCTCGTA232780.18637006940559733No Hit
CAACACCAGTCGATGGGCTGTCATCTCGTA231900.1856655172057652No Hit
TGACCTATGAATTGACAGCCAGTATCTCGT209490.16772345493503987No Hit
ATGACCTATGAATTGACAGACAATCTCGTA205580.1645930014107857No Hit
TGTAAACATCCTCGACTGGAAGCATCTCGT203660.16305579661115194No Hit
TGGCTCAGTTCAGCAGGAACAGTATCTCGT202140.1618388428114419No Hit
ACTGGACTTGGAGTCAGAAGGCATCTCGTA202110.16181482398644761No Hit
GTACAGTACTGTGATAACTGAATCTCGTAT201410.16125438473658113No Hit
TGTAACAGCAACTCCATGTGGATCTCGTAT196850.15760352333745095No Hit
TTTGGCAATGGTAGAACTCACACTATCTCG192850.15440101333821396No Hit
TTTGGCAATGGTAGAACTCACAATCTCGTA183440.14686710856500892No Hit
CGGCGGCGGCGACTCTGGACGCGATCTCGT180290.14434513194060977No Hit
TTTGTTCGTTCGGCTCGCGTGATCTCGTAT176750.141510910591285No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGT173670.13904497789187253No Hit
TGTAAACATCCTCGACTGGAAGAATCTCGT166490.13329647244324214No Hit
GTCTACGGCCATACCACCCTGAACGCGCCC166440.13325644106825166No Hit
CTGACCTATGAATTGACAGCCAGATCTCGT166080.13296821516832033No Hit
TACCCTGTAGAACCGAATTTGTGTATCTCG164920.1320394872685416No Hit
TGAGATGAAGCACTGTAGCATCTCGTATGC160920.1288369772693046No Hit
TGAGATGAAGCACTGTAGCTCTGATCTCGT160870.12879694589431412No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAA158510.1269074649947643Illumina Single End Adapter 1 (95% over 23bp)
GGCGGCGGCGGCGACTCTGGACGCGATCTC157510.12610683749495505No Hit
CACCCGTAGAACCGACCTTGCGATCTCGTA156890.1256104484450733No Hit
CACCCGTAGAACCGACCTTGCATCTCGTAT154700.12385707422049104No Hit
TGAGATGAAGCACTGTAGCAATCTCGTATG154550.12373698009551966No Hit
GTGAAATGTTTAGGACCACTAGAATCTCGT153870.12319255339564936No Hit
TACCCTGTAGATCCGAATTTGTGAATCTCG153260.12270417062076573No Hit
CGGCGGCGGCGGCGACTCTGGACGCATCTC149290.11952567944652301No Hit
TGAGATGAAGCACTGTAGAAATCTCGTATG149070.11934954139656496No Hit
AAGCTGCCAGTTGAAGAACTGTAATCTCGT142920.11442568227273808No Hit
GTCCAGTTTTCCCAGGAATCCCTATCTCGT141360.11317670337303565No Hit
TAGCACCATTTGAAATCGGTTAATCTCGTA140440.11244012607321113No Hit
TACCCTGTAGATCCGAATTTGATCTCGTAT134340.1075562983243747No Hit
TTCAAGTAATCCAGGATAGGCTATCTCGTA133720.10705990927449298No Hit
CTTTCAGTCGGATGTTTACAGATCTCGTAT132750.10628330059967801No Hit
TTTGTTCGTTCGGCTCGCGTGAAATCTCGT131440.10523447857492788No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTAT128570.10293667765047534No Hit
TAATACTGCCTGGTAATGATGACATCTCGT127120.10177576777575191No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)