FastQCFastQC Report
Wed 8 May 2024
EGAF00001082978

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001082978
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7572982
Total Bases227.1 Mbp
Sequences flagged as poor quality0
Sequence length30
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTATGCCGTCTTCTGCTTGAAAAAA79650810.517759054491348Illumina Paired End PCR Primer 2 (100% over 24bp)
TACCCTGTAGAACCGAATTTGTATCTCGTA4615236.094336418599701No Hit
TGAGATGAAGCACTGTAGCTCTATCTCGTA3533174.6654937249289645No Hit
TAGCTTATCAGACTGATGTTGAATCTCGTA3329884.397052574534047No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTA2911553.8446545891697617No Hit
TGACCTATGAATTGACAGCCAGATCTCGTA2817853.7209252577122194No Hit
TTTGTTCGTTCGGCTCGCGTGAATCTCGTA2746153.6262465697132256No Hit
TACCCTGTAGATCCGAATTTGTATCTCGTA2405923.176978368626784No Hit
TCAGTGCACTACAGAACTTTGATCTCGTAT1689432.2308649353715615No Hit
TGAGATGAAGCACTGTAGCTATCTCGTATG1347811.7797612618120577No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTA1258191.6614195042322828No Hit
TGTAACAGCAACTCCATGTGGAATCTCGTA1236311.632527318828963No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGT1036991.3693284890945205No Hit
CTGACCTATGAATTGACAGCCATCTCGTAT904361.1941927235532845No Hit
TACCCTGTAGAACCGAATTTGTGATCTCGT726370.9591598131356973No Hit
TGAGATGAAGCACTGTAGCTCATCTCGTAT712880.9413464867604334No Hit
TCAGTGCACTACAGAACTTTGAATCTCGTA671020.8860710351615784No Hit
CTGACCTATGAATTGACAGCCAATCTCGTA583230.7701457629240371No Hit
TAATACTGCCGGGTAATGATGGAATCTCGT503630.665035252955837No Hit
TAGCTTATCAGACTGATGTTGACATCTCGT471250.622277987720029No Hit
TGTAAACATCCTCGACTGGAAGATCTCGTA421860.5570592931555891No Hit
TATTGCACTTGTCCCGGCCTGTATCTCGTA421540.5566367383416467No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGT413390.5458747954240483No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTA352930.46603834526478477No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTA304350.40188924257313696No Hit
ACCCTGTAGATCCGAATTTGTATCTCGTAT293470.3875223788990915No Hit
GTGAAATGTTTAGGACCACTAGATCTCGTA290670.38382502427709453No Hit
TAGCTTATCAGACTGATGTTGATCTCGTAT285220.3766283876021361No Hit
TACAGTACTGTGATAACTGAATCTCGTATG268310.35429900665286146No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAA267180.35280685996612693Illumina Paired End PCR Primer 2 (100% over 23bp)
CACTAGATTGTGAGCTCCTGGAATCTCGTA261090.3447651136632835No Hit
ACCCTGTAGAACCGAATTTGTGATCTCGTA234110.3091384609127554No Hit
CACCCGTAGAACCGACCTTGCGATCTCGTA233210.30795002549854206No Hit
TAGCACCATCTGAAATCGGTTATCTCGTAT231710.3059692998081865No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTA227010.2997630259784059No Hit
TACCCTGTAGAACCGAATTTGATCTCGTAT213830.2823590495791486No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTA206550.2727459275619564No Hit
GTCTACGGCCATACCACCCTGAACGCGCCC201270.265773773131905No Hit
ACCCTGTAGATCCGAATTTGTGAATCTCGT200510.2647702054487915No Hit
TACAGTACTGTGATAACTGAATATCTCGTA197800.2611916943682159No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGT194620.25699255590466213No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTA173680.22934162526729893No Hit
ACCCTGTAGAACCGAATTTGTATCTCGTAT165280.21824956140130797No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTA162020.2139447842342686No Hit
TGAGATGAAGCACTGTAGCTCTTATCTCGT159870.21110574407809235No Hit
TGAGATGAAGCACTGTAGCTCAATCTCGTA150410.1986139673909168No Hit
TAATACTGCCGGGTAATGATGGATCTCGTA149600.19754437551812482No Hit
CACCCGTAGAACCGACCTTGCATCTCGTAT138930.18345481344072917No Hit
TGAAATGTTTAGGACCACTAGATCTCGTAT138700.18315110216820799No Hit
TGAGATGAAGCACTGTAGCTCTAATCTCGT136960.18085346036739558No Hit
TGTAACAGCAACTCCATGTGGAAATCTCGT131790.17402655915463686No Hit
AACCCGTAGATCCGAACTTGTGAATCTCGT130840.172772099550745No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCAT127840.16881064817003397No Hit
TTTGTTCGTTCGGCTCGCGTGATCTCGTAT127710.1686389852768698No Hit
TACCCTGTAGAACCGAATTTGTGAATCTCG127250.16803156273182743No Hit
CGCGACCTCAGATCAGACGTGGATCTCGTA124860.1648756064651943No Hit
GTACAGTACTGTGATAACTGAATCTCGTAT124840.1648491967893229No Hit
CTGACCTATGAATTGACAGCCAGATCTCGT121540.1604916002705407No Hit
TGTAACAGCAACTCCATGTGGATCTCGTAT118970.15709795692106493No Hit
TCGTACCGTGAGTAATAATGCGATCTCGTA118790.15686026983822224No Hit
CAACACCAGTCGATGGGCTGTCATCTCGTA115900.15304407167480394No Hit
TCAGTGCACTACAGAACTTTGTCATCTCGT115720.15280638459196128No Hit
ACCCTGTAGAACCGAATTTGTGTATCTCGT115660.15272715556434704No Hit
ACCCTGTAGAACCGAATTTGTGAATCTCGT114350.15099732179476988No Hit
TTTGGCAATGGTAGAACTCACACTATCTCG112720.1488449332112502No Hit
AAGCTGCCAGTTGAAGAACTGTAATCTCGT107240.14160868202248467No Hit
GTGAAATGTTTAGGACCACTAGAATCTCGT106970.14125215139822067No Hit
TCGTTTCCGGCTCGAAGGACCAATCTCGTA105280.1390205337870868No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTAT104500.1379905564281019No Hit
TGAGATGAAGCACTGTAGCTAATCTCGTAT103390.13652481941723882No Hit
TACCCTGTAGATCCGAATTTGTGAATCTCG102060.13476857597179023No Hit
CTGACCTATGAATTGACAGCCATATCTCGT101270.13372539377486967No Hit
TGAGATGAAGCACTGTAGATCTCGTATGCC100150.13224645192607087No Hit
TGACCTATGAATTGACAGCCAGTATCTCGT95140.1256308281202834No Hit
TTTGTTCGTTCGGCTCGCGTGAAATCTCGT94550.12485174268207688No Hit
ACTGGACTTGGAGTCAGAAGGCATCTCGTA93900.12399342821625615No Hit
CATCTTACCGGACAGTGCTGGAATCTCGTA93690.12371612661960638No Hit
TACCCTGTAGATCCGAATTTGATCTCGTAT93210.1230822943986926No Hit
TAGCACCATTTGAAATCGGTTATCTCGTAT89730.11848701079706779No Hit
CTTTCAGTCGGATGTTTACAGATCTCGTAT82110.1084249242900617No Hit
CGGCGACTCTGGACGCGAGCCGGGATCTCG81540.10767224852772658No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC78900.10418617131270086No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)