FastQCFastQC Report
Thu 9 May 2024
EGAF00001082979

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001082979
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12977203
Total Bases389.3 Mbp
Sequences flagged as poor quality0
Sequence length30
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTATGCCGTCTTCTGCTTGAAAAAA130177910.031275614629747Illumina Paired End PCR Primer 2 (100% over 24bp)
TAGCTTATCAGACTGATGTTGAATCTCGTA7700595.933936611764492No Hit
TGAGATGAAGCACTGTAGCTCTATCTCGTA6571755.064072743564233No Hit
TGACCTATGAATTGACAGCCAGATCTCGTA6465784.982414161202533No Hit
TACCCTGTAGAACCGAATTTGTATCTCGTA5384814.149438056875584No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTA5251124.0464189394278565No Hit
TTTGTTCGTTCGGCTCGCGTGAATCTCGTA4149963.197884783030673No Hit
TGTAACAGCAACTCCATGTGGAATCTCGTA2884282.222574463850184No Hit
TACCCTGTAGATCCGAATTTGTATCTCGTA2839862.1883452081315213No Hit
TGAGATGAAGCACTGTAGCTATCTCGTATG2601472.004646147555833No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTA2368431.8250697010750314No Hit
TCAGTGCACTACAGAACTTTGATCTCGTAT2313251.7825489822421672No Hit
CTGACCTATGAATTGACAGCCATCTCGTAT1724041.3285143185322754No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGT1683781.2974906842406642No Hit
TGAGATGAAGCACTGTAGCTCATCTCGTAT1333001.0271859043894127No Hit
CTGACCTATGAATTGACAGCCAATCTCGTA1210240.9325892490084342No Hit
TAGCTTATCAGACTGATGTTGACATCTCGT1190290.9172161366359146No Hit
TAATACTGCCGGGTAATGATGGAATCTCGT1103710.8504991406854003No Hit
TACCCTGTAGAACCGAATTTGTGATCTCGT908200.6998426394347071No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTA819120.6311991882996667No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGT695560.5359860672596399No Hit
TATTGCACTTGTCCCGGCCTGTATCTCGTA656880.5061799526446492No Hit
TCAGTGCACTACAGAACTTTGAATCTCGTA573500.4419288193303287No Hit
TAGCTTATCAGACTGATGTTGATCTCGTAT534950.4122228803849335No Hit
GTGAAATGTTTAGGACCACTAGATCTCGTA492870.37979678671898714No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAA447050.3444887160969895Illumina Paired End PCR Primer 2 (100% over 23bp)
TGTAAACATCCTCGACTGGAAGATCTCGTA438360.33779235787557615No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTA424030.32674991675787146No Hit
CACTAGATTGTGAGCTCCTGGAATCTCGTA403970.3112920403572326No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTA398290.30691513417798894No Hit
GTCTACGGCCATACCACCCTGAACGCGCCC385940.2973984455664291No Hit
TACAGTACTGTGATAACTGAATCTCGTATG380460.29317565580194743No Hit
ACCCTGTAGATCCGAATTTGTATCTCGTAT365050.28130098604452747No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTA340870.2626683114997893No Hit
TAATACTGCCGGGTAATGATGGATCTCGTA334190.2575208232467351No Hit
GCGGCGACTCTGGACGCGAGCATCTCGTAT323710.24944512311320088No Hit
TGACCTATGAATTGACAGCCAGTATCTCGT317970.24502198200952854No Hit
CACCCGTAGAACCGACCTTGCGATCTCGTA317730.24483704231181405No Hit
TGAGATGAAGCACTGTAGCTCTTATCTCGT315750.2433112898056692No Hit
TAGCACCATCTGAAATCGGTTATCTCGTAT304140.23436483192872917No Hit
TGTAACAGCAACTCCATGTGGAAATCTCGT302790.233324546129085No Hit
ACCCTGTAGAACCGAATTTGTGATCTCGTA299460.23075850782329596No Hit
TGAGATGAAGCACTGTAGCTCAATCTCGTA298860.23029615857900967No Hit
TACAGTACTGTGATAACTGAATATCTCGTA275120.2120025401467481No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGT274460.21149395597803317No Hit
ACCCTGTAGATCCGAATTTGTGAATCTCGT271800.20944420766169722No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTA263470.20302525898685564No Hit
TGAAATGTTTAGGACCACTAGATCTCGTAT256290.19749247969689615No Hit
TACCCTGTAGAACCGAATTTGATCTCGTAT250550.19306933859322384No Hit
TGTAACAGCAACTCCATGTGGATCTCGTAT246280.18977895313805293No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTAT242490.1868584470783111No Hit
CTGACCTATGAATTGACAGCCAGATCTCGT237500.18301324252999665No Hit
TGAGATGAAGCACTGTAGCTCTAATCTCGT235810.18171095882525687No Hit
CTGACCTATGAATTGACAGCCATATCTCGT234280.18053196825232679No Hit
ATGACCTATGAATTGACAGACAATCTCGTA230300.17746505159856096No Hit
ACTGGACTTGGAGTCAGAAGGCATCTCGTA222660.17157780455464863No Hit
TCAGTGCACTACAGAACTTTGTCATCTCGT209870.1617220598306122No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTA207530.15991889777789559No Hit
GTACAGTACTGTGATAACTGAATCTCGTAT206590.1591945506285137No Hit
TTTGTTCGTTCGGCTCGCGTGATCTCGTAT204120.15729121290620174No Hit
ACCCTGTAGAACCGAATTTGTATCTCGTAT200280.15433217774276936No Hit
TGAGATGAAGCACTGTAGCTAATCTCGTAT181300.13970652998184585No Hit
TTTGTTCGTTCGGCTCGCGTGAAATCTCGT177720.13694784615760422No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC171140.13187741611193105No Hit
AACCCGTAGATCCGAACTTGTGAATCTCGT167570.12912643810842753No Hit
AAGCTGCCAGTTGAAGAACTGTAATCTCGT166400.12822485708206924No Hit
CGGCGGCGGCGACTCTGGACGCGAGCATCT163600.1260672272753998No Hit
TACCCTGTAGAACCGAATTTGTGAATCTCG162340.12509629386239854No Hit
CACCCGTAGAACCGACCTTGCATCTCGTAT157590.1214360290117986No Hit
CAACACCAGTCGATGGGCTGTCATCTCGTA157330.12123567767260787No Hit
TCGTACCGTGAGTAATAATGCGATCTCGTA154020.11868505100829509No Hit
ACCCTGTAGAACCGAATTTGTGTATCTCGT149970.11556419360936251No Hit
CGGCGACTCTGGACGCGAGCATCTCGTATG149770.1154100771946004No Hit
GTGAAATGTTTAGGACCACTAGAATCTCGT149340.11507872690286189No Hit
GGCGGCGGCGACTCTGGACGCGAGCATCTC148150.11416173423502737No Hit
TCCAGCATCAGTGATTTTGTTGAATCTCGT148120.11413861677281305No Hit
GGCGGCGACTCTGGACGCGAGCATCTCGTA147210.1134373870856455No Hit
GTCCAGTTTTCCCAGGAATCCCTATCTCGT146510.11289797963397814No Hit
TAACACTGTCTGGTAAAGATGGCATCTCGT145750.11231233725788214No Hit
TGAGATGAAGCACTGTAGATCTCGTATGCC143140.1103011180452367No Hit
TAGCACCATTTGAAATCGGTTATCTCGTAT142660.10993123864980767No Hit
ACCCTGTAGAACCGAATTTGTGAATCTCGT140540.10829760465332937No Hit
TACCCTGTAGATCCGAATTTGTGAATCTCG137920.10627867961994583No Hit
CGGCGGCGACTCTGGACGCGAGCATCTCGT137910.10627097379920775No Hit
TACCCTGTAGATCCGAATTTGATCTCGTAT134580.10370493549341872No Hit
TTTGGCAATGGTAGAACTCACACTATCTCG132770.1023101819398217No Hit
TGAAATGTTTAGGACCACTAGTATCTCGTA130290.10039913839677161No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)