FastQCFastQC Report
Wed 8 May 2024
EGAF00001082980

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001082980
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13661580
Total Bases409.8 Mbp
Sequences flagged as poor quality0
Sequence length30
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCTTATCAGACTGATGTTGAATCTCGTA12893539.437802948121666No Hit
ATCTCGTATGCCGTCTTCTGCTTGAAAAAA12001168.784606172931682Illumina Paired End PCR Primer 2 (100% over 24bp)
TGAGATGAAGCACTGTAGCTCTATCTCGTA8646086.328755531937008No Hit
TTTGTTCGTTCGGCTCGCGTGAATCTCGTA8399966.148600674299751No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTA6929155.071997528836342No Hit
TGACCTATGAATTGACAGCCAGATCTCGTA4991783.6538819082419454No Hit
TGTAACAGCAACTCCATGTGGAATCTCGTA4383313.208494185884795No Hit
TACCCTGTAGATCCGAATTTGTATCTCGTA3720692.723469759720325No Hit
TACCCTGTAGAACCGAATTTGTATCTCGTA3394862.484968795702986No Hit
TGAGATGAAGCACTGTAGCTATCTCGTATG2824522.0674914614561417No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTA2427411.7768149804049018No Hit
CTGACCTATGAATTGACAGCCATCTCGTAT2045301.4971181956991797No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGT2012461.4730799805000594No Hit
TGAGATGAAGCACTGTAGCTCATCTCGTAT1636821.198119104818037No Hit
TCAGTGCACTACAGAACTTTGATCTCGTAT1495531.0946976850408225No Hit
TAGCTTATCAGACTGATGTTGACATCTCGT1307500.9570635314509742No Hit
TAGCTTATCAGACTGATGTTGATCTCGTAT1288590.943221794258058No Hit
CTGACCTATGAATTGACAGCCAATCTCGTA1237030.9054809180197312No Hit
TAATACTGCCGGGTAATGATGGAATCTCGT1099510.8048190619240234No Hit
TCAGTGCACTACAGAACTTTGAATCTCGTA921900.6748121373955281No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTA755850.5532668988506454No Hit
GTGAAATGTTTAGGACCACTAGATCTCGTA691360.5060615243624823No Hit
TGTAACAGCAACTCCATGTGGATCTCGTAT684200.5008205493068884No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTA659460.48271137013434756No Hit
TTTGTTCGTTCGGCTCGCGTGATCTCGTAT608100.44511688984729436No Hit
TATTGCACTTGTCCCGGCCTGTATCTCGTA549420.40216431774362843No Hit
CGCGACCTCAGATCAGACGTGGATCTCGTA518870.37980233618659043No Hit
TGTAAACATCCTCGACTGGAAGATCTCGTA513210.37565933076554836No Hit
ACCCTGTAGATCCGAATTTGTATCTCGTAT493960.36156872045546706No Hit
TAATACTGCCGGGTAATGATGGATCTCGTA473090.3462923029400699No Hit
TACCCTGTAGAACCGAATTTGTGATCTCGT451900.330781651902635No Hit
TACAGTACTGTGATAACTGAATCTCGTATG435520.31879182349332946No Hit
TGAGATGAAGCACTGTAGCTCTTATCTCGT432090.31628113292898774No Hit
TGAGATGAAGCACTGTAGCTCAATCTCGTA409980.30009706051569435No Hit
TGACCTATGAATTGACAGCCAGTATCTCGT404180.2958515779287608No Hit
TAGCACCATCTGAAATCGGTTATCTCGTAT400100.29286510052278No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTA397000.2905959632780396No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTA390950.286167485752014No Hit
TTTGTTCGTTCGGCTCGCGTGAAATCTCGT386340.28279305907515817No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAA383030.2803702060815806Illumina Paired End PCR Primer 2 (100% over 23bp)
CACTAGATTGTGAGCTCCTGGAATCTCGTA373790.2736067131327416No Hit
ACCCTGTAGATCCGAATTTGTGAATCTCGT371240.2717401647540036No Hit
AACCCGTAGATCCGAACTTGTGAATCTCGT369500.2704665199779235No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGT359210.2629344482848982No Hit
TGAGATGAAGCACTGTAGCTCTAATCTCGT345730.25306736116905953No Hit
GTGAAATGTTTAGGACCACTAGAATCTCGT338810.24800206125499394No Hit
CACCCGTAGAACCGACCTTGCGATCTCGTA336990.24666985809840442No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGT335060.24525713716861447No Hit
GTCTACGGCCATACCACCCTGAACGCGCCC331030.242307258750452No Hit
TACAGTACTGTGATAACTGAATATCTCGTA326920.2392988219517801No Hit
CGCGACCTCAGATCAGACGTGGCGATCTCG323980.2371468014680586No Hit
TGAAATGTTTAGGACCACTAGATCTCGTAT312550.22878027285277397No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTAT295060.21597794691390015No Hit
AAGCTGCCAGTTGAAGAACTGTAATCTCGT284550.20828483967447395No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC283670.20764069748887026No Hit
TACCCTGTAGATCCGAATTTGATCTCGTAT283330.2073918243717052No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTA270240.19781020936084992No Hit
ATGACCTATGAATTGACAGACAATCTCGTA266910.19537271677214493No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTA265220.19413567098388326No Hit
TGTAACAGCAACTCCATGTGGAAATCTCGT242050.17717570002884No Hit
CTGACCTATGAATTGACAGCATCTCGTATG241100.17648031926029054No Hit
CTGACCTATGAATTGACAGCCATATCTCGT240870.17631196391632592No Hit
GTACAGTACTGTGATAACTGAATCTCGTAT235110.17209576051964706No Hit
CTGACCTATGAATTGACAGCCAGATCTCGT230210.16850905971344457No Hit
TGAAATGTTTAGGACCACTAGTATCTCGTA222300.1627190998405748No Hit
TGAGATGAAGCACTGTAGCTAATCTCGTAT215370.15764648012894555No Hit
TCAGTGCACTACAGAACTTTGTCATCTCGT214870.15728049025076163No Hit
CACCCGTAGAACCGACCTTGCATCTCGTAT213460.15624839879428296No Hit
CGCGACCTCAGATCAGACGTGGCATCTCGT199480.1460153218002603No Hit
TAATACTGCCTGGTAATGATGAATCTCGTA198750.14548097657811176No Hit
TTTGTTCGTTCGGCTCGCGTGATATCTCGT198350.14518818467556463No Hit
ACTGGACTTGGAGTCAGAAGGCATCTCGTA188140.1377146713630488No Hit
AACCCGTAGATCCGAACTTGTATCTCGTAT182310.13344722938342415No Hit
TAGCTTATCAGACTGATGTTGAAATCTCGT181810.13308123950524026No Hit
TGTAAACATCCTCGACTGGAAGCATCTCGT180820.13235657954643607No Hit
TCCAGCATCAGTGATTTTGTTGAATCTCGT180260.13194667088287007No Hit
TTTGTTCGTTCGGCTCGCGTGTATCTCGTA179750.13157336120712246No Hit
TACCCTGTAGAACCGAATTTGATCTCGTAT179000.13102437638984654No Hit
TACCCTGTAGATCCGAATTTGTGAATCTCG174400.12765726951055442No Hit
TGACCTATGAATTGACAGCCAGAATCTCGT168410.12327271076991095No Hit
TGAAATGTTTAGGACCACTAGAATCTCGTA167810.12283352291609023No Hit
TTGTTCGTTCGGCTCGCGTGAATCTCGTAT167140.12234309647932376No Hit
TTTGGCAATGGTAGAACTCACAATCTCGTA161680.11834648700955526No Hit
ACCCTGTAGAACCGAATTTGTGATCTCGTA155380.11373501454443775No Hit
TGTAAACATCCTTGACTGGAAGATCTCGTA149400.10935777560135797No Hit
TTTGGCAATGGTAGAACTCACACTATCTCG144170.10552952147555407No Hit
ACCCTGTAGAACCGAATTTGTATCTCGTAT140260.10266748062815576No Hit
TGAGGTAGTAGGTTGTATAGATCTCGTATG136880.10019338905163239No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)