FastQCFastQC Report
Wed 8 May 2024
EGAF00001082981

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001082981
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8049475
Total Bases241.4 Mbp
Sequences flagged as poor quality0
Sequence length30
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTATGCCGTCTTCTGCTTGAAAAAA6889728.559216594871094Illumina Paired End PCR Primer 2 (100% over 24bp)
TTTGTTCGTTCGGCTCGCGTGAATCTCGTA6619828.223915224284815No Hit
TAGCTTATCAGACTGATGTTGAATCTCGTA6097687.575251802136164No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTA4644935.770475714254656No Hit
TGACCTATGAATTGACAGCCAGATCTCGTA3388104.209094381931741No Hit
TCAGTGCACTACAGAACTTTGATCTCGTAT2462113.058721220949192No Hit
TACCCTGTAGAACCGAATTTGTATCTCGTA2298872.855925386438246No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTA2081272.585597197332745No Hit
TGTAACAGCAACTCCATGTGGAATCTCGTA1862412.313703688749887No Hit
TACCCTGTAGATCCGAATTTGTATCTCGTA1845732.292981840430587No Hit
TGAGATGAAGCACTGTAGCTCTATCTCGTA1713992.129318992853571No Hit
CTGACCTATGAATTGACAGCCATCTCGTAT1566191.9457045335254786No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGT1083911.34655986881132No Hit
TGAGATGAAGCACTGTAGCTATCTCGTATG1078131.339379276288205No Hit
CTGACCTATGAATTGACAGCCAATCTCGTA883531.0976243792297014No Hit
TCAGTGCACTACAGAACTTTGAATCTCGTA871301.0824308417629722No Hit
TAGCTTATCAGACTGATGTTGACATCTCGT680680.8456203665456442No Hit
GTGAAATGTTTAGGACCACTAGATCTCGTA655140.8138915892030226No Hit
TAGCTTATCAGACTGATGTTGATCTCGTAT647330.8041890930774988No Hit
TAATACTGCCGGGTAATGATGGAATCTCGT637910.7924864665086854No Hit
TTTGTTCGTTCGGCTCGCGTGATCTCGTAT534770.6643538864335873No Hit
TGAGATGAAGCACTGTAGCTCATCTCGTAT444400.5520856950298001No Hit
TACCCTGTAGAACCGAATTTGTGATCTCGT391110.4858826196739539No Hit
TATTGCACTTGTCCCGGCCTGTATCTCGTA389520.4839073355715745No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTA375640.46666397497973466No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTA323280.4016162544762236No Hit
CGCGACCTCAGATCAGACGTGGATCTCGTA293060.36407343336056075No Hit
GTGAAATGTTTAGGACCACTAGAATCTCGT292250.36306715655368826No Hit
TAGCACCATCTGAAATCGGTTATCTCGTAT270690.33628280105224256No Hit
TGAAATGTTTAGGACCACTAGATCTCGTAT267870.3327794669838716No Hit
CACTAGATTGTGAGCTCCTGGAATCTCGTA266430.3309905304383205No Hit
TGTAACAGCAACTCCATGTGGATCTCGTAT265310.3295991353473363No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGT263670.3275617353926809No Hit
ACCCTGTAGATCCGAATTTGTATCTCGTAT262910.3266175744380845No Hit
CACCCGTAGAACCGACCTTGCGATCTCGTA252140.3132378198578168No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTA247810.3078585870507083No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAA238990.29690135070920776Illumina Paired End PCR Primer 2 (100% over 23bp)
TTTGTTCGTTCGGCTCGCGTGAAATCTCGT236410.293696172731762No Hit
TAATACTGCCGGGTAATGATGGATCTCGTA231330.2873852021405123No Hit
TGTAAACATCCTCGACTGGAAGATCTCGTA213030.26465080020746695No Hit
TTTGGCAATGGTAGAACTCACAATCTCGTA201180.24992934321803598No Hit
AAGCTGCCAGTTGAAGAACTGTAATCTCGT186640.23186605337615188No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGT180640.22441215110302223No Hit
TGACCTATGAATTGACAGCCAGTATCTCGT179030.22241202065973248No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTA176140.21882172439817502No Hit
CTGACCTATGAATTGACAGCCATATCTCGT173520.21556685373890846No Hit
CTGACCTATGAATTGACAGCATCTCGTATG171700.21330583671605913No Hit
TCAGTGCACTACAGAACTTTGTCATCTCGT170070.2112808599318589No Hit
TACCCTGTAGAACCGAATTTGATCTCGTAT168490.20931799899993478No Hit
TACAGTACTGTGATAACTGAATCTCGTATG167700.2083365685339727No Hit
TGTAACAGCAACTCCATGTGGAAATCTCGT162460.2018268272154395No Hit
TACCCTGTAGATCCGAATTTGATCTCGTAT161170.20022423822671667No Hit
TTTGGCAATGGTAGAACTCACACTATCTCG161120.20016212237444056No Hit
ACCCTGTAGATCCGAATTTGTGAATCTCGT155890.1936648042263626No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTAT142890.17751468263458176No Hit
CTGACCTATGAATTGACAGCCAGATCTCGT142690.17726621922547744No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTA139520.17332807419117396No Hit
ACCCTGTAGAACCGAATTTGTGATCTCGTA131190.162979573201979No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTA128510.1596501635199811No Hit
TTGTTCGTTCGGCTCGCGTGAATCTCGTAT126210.15679283431528143No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC121090.15043217104221085No Hit
TTTGTTCGTTCGGCTCGCGTGTATCTCGTA119740.14875504303075668No Hit
CAACACCAGTCGATGGGCTGTCATCTCGTA119400.1483326552352793No Hit
TACAGTACTGTGATAACTGAATATCTCGTA118260.1469164138033847No Hit
TGAAATGTTTAGGACCACTAGTATCTCGTA117630.14613375406470608No Hit
TGAAATGTTTAGGACCACTAGAATCTCGTA115970.14407150776914024No Hit
CACCCGTAGAACCGACCTTGCATCTCGTAT110210.13691576158693577No Hit
ATGACCTATGAATTGACAGACAATCTCGTA110140.13682879939374928No Hit
CGCGACCTCAGATCAGACGTGGCGATCTCG108700.1350398628481982No Hit
CATCTTACCGGACAGTGCTGGAATCTCGTA106210.1319464934048494No Hit
TTTTGTTCGTTCGGCTCGCGTGAATCTCGT104880.13029421173430566No Hit
TTTGTTCGTTCGGCTCGCGTGATATCTCGT104220.12947428248426138No Hit
TCAGTGCACTACAGAACTTTGTTATCTCGT103220.1282319654387398No Hit
GTCTACGGCCATACCACCCTGAACGCGCCC100880.12532494355221924No Hit
TGAGGTAGTAGGTTGTGTGGTTATCTCGTA100740.12515101916584623No Hit
TAATACTGCCTGGTAATGATGAATCTCGTA100680.12507648014311493No Hit
ACCCTGTAGAACCGAATTTGTATCTCGTAT93790.11651691569947108No Hit
GTACAGTACTGTGATAACTGAATCTCGTAT92200.11454163159709174No Hit
TAGCACCATTTGAAATCGGTTATCTCGTAT88710.11020594510822135No Hit
TGAGGTAGTAGGTTGTATAGATCTCGTATG86950.10801946710810333No Hit
TCCAGCATCAGTGATTTTGTTGAATCTCGT85340.10601933666481356No Hit
TGAGATGAAGCACTGTAGCTCTTATCTCGT85050.10565906472161228No Hit
TGTAAACATCCTTGACTGGAAGATCTCGTA83000.10311231477829298No Hit
TAGCTTATCAGACTGATGTTGAAATCTCGT82890.10297565990328562No Hit
TGAGATGAAGCACTGTAGCTCAATCTCGTA81680.10147245627820448No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)