FastQCFastQC Report
Wed 8 May 2024
EGAF00001082982

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001082982
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16485115
Total Bases494.5 Mbp
Sequences flagged as poor quality0
Sequence length30
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCTTATCAGACTGATGTTGAATCTCGTA15384819.332546360762422No Hit
ATCTCGTATGCCGTCTTCTGCTTGAAAAAA14366478.714813333118999Illumina Paired End PCR Primer 2 (100% over 24bp)
TGAGATGAAGCACTGTAGCTCTATCTCGTA12680137.691866268448839No Hit
TGACCTATGAATTGACAGCCAGATCTCGTA6182123.750122458957672No Hit
TACCCTGTAGAACCGAATTTGTATCTCGTA6127673.717092662077274No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTA5957183.613672091459477No Hit
TTTGTTCGTTCGGCTCGCGTGAATCTCGTA4793892.908011257428292No Hit
TACCCTGTAGATCCGAATTTGTATCTCGTA4561672.7671447848559136No Hit
TGTAACAGCAACTCCATGTGGAATCTCGTA4066552.466801111184241No Hit
TGAGATGAAGCACTGTAGCTATCTCGTATG3933622.3861647310316No Hit
TCAGTGCACTACAGAACTTTGATCTCGTAT3538832.1466820219331195No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTA3507212.1275010820367344No Hit
TGAGATGAAGCACTGTAGCTCATCTCGTAT2664711.6164339769543616No Hit
CTGACCTATGAATTGACAGCCATCTCGTAT2028181.2303098886480317No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGT1957091.1871861373123573No Hit
TAGCTTATCAGACTGATGTTGACATCTCGT1819261.1035773787444005No Hit
TAATACTGCCGGGTAATGATGGAATCTCGT1167450.7081843226450043No Hit
TCAGTGCACTACAGAACTTTGAATCTCGTA1165110.7067648602997311No Hit
CTGACCTATGAATTGACAGCCAATCTCGTA1050730.6373810555765004No Hit
TAGCTTATCAGACTGATGTTGATCTCGTAT996080.6042299371281304No Hit
TACCCTGTAGAACCGAATTTGTGATCTCGT871000.5283554285183938No Hit
CGCGACCTCAGATCAGACGTGGATCTCGTA696820.4226964749715122No Hit
TGTAAACATCCTCGACTGGAAGATCTCGTA625830.3796333844198236No Hit
TGAGATGAAGCACTGTAGCTCTTATCTCGT615500.37336712543406586No Hit
TGAGATGAAGCACTGTAGCTCAATCTCGTA590340.35810487218317855No Hit
TGTAACAGCAACTCCATGTGGATCTCGTAT558280.33865702483725474No Hit
ACCCTGTAGATCCGAATTTGTATCTCGTAT551150.3343319109390502No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTA506800.30742885324124214No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTA490130.2973167005507696No Hit
TACAGTACTGTGATAACTGAATCTCGTATG488540.2963521940853916No Hit
TAATACTGCCGGGTAATGATGGATCTCGTA476240.2888909176551089No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTA471980.2863067682573036No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTA428040.2596524197738384No Hit
TATTGCACTTGTCCCGGCCTGTATCTCGTA418920.2541201562743117No Hit
GTCTACGGCCATACCACCCTGAACGCGCCC417780.2534286233368709No Hit
CACTAGATTGTGAGCTCCTGGAATCTCGTA411440.24958272963215605No Hit
ACCCTGTAGATCCGAATTTGTGAATCTCGT406720.24671954062801504No Hit
TGAGATGAAGCACTGTAGCTCTAATCTCGT382460.2320032344330021No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTA381860.2316392697290859No Hit
CAACACCAGTCGATGGGCTGTCATCTCGTA371280.22522135878336305No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAA370750.22489985662823708Illumina Paired End PCR Primer 2 (100% over 23bp)
TGACCTATGAATTGACAGCCAGTATCTCGT370380.2246754117274887No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGT367110.22269180409114525No Hit
TACAGTACTGTGATAACTGAATATCTCGTA359120.21784500745066082No Hit
TGTAACAGCAACTCCATGTGGAAATCTCGT357100.21661965961414284No Hit
TAGCACCATCTGAAATCGGTTATCTCGTAT345960.20986204827809818No Hit
ATGACCTATGAATTGACAGACAATCTCGTA337610.20479687281526396No Hit
GCGGCGACTCTGGACGCGAGCATCTCGTAT335770.20368071438992083No Hit
TACCCTGTAGAACCGAATTTGATCTCGTAT326150.197845146970464No Hit
TTTGTTCGTTCGGCTCGCGTGATCTCGTAT323500.19623763619483395No Hit
TACCCTGTAGATCCGAATTTGATCTCGTAT318600.19326525777951806No Hit
GTGAAATGTTTAGGACCACTAGATCTCGTA306090.18567659370286468No Hit
GCGGCGGCGGCGACTCTGGACGCGAGCATC291510.17683225139770029No Hit
ACCCTGTAGAACCGAATTTGTGATCTCGTA290170.17601939689228738No Hit
TGAGATGAAGCACTGTAGCTAATCTCGTAT288050.17473338827178336No Hit
CTGACCTATGAATTGACAGCATCTCGTATG278990.1692375212426483No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTA272770.1654644204787167No Hit
TCAGTGCACTACAGAACTTTGTCATCTCGT266090.16141228010844935No Hit
GTACAGTACTGTGATAACTGAATCTCGTAT263290.1597137781568403No Hit
CTGACCTATGAATTGACAGCCAGATCTCGT260360.15793641718604934No Hit
GGCGGCGGCGACTCTGGACGCGAGCATCTC246400.1494681717415984No Hit
AACCCGTAGATCCGAACTTGTGAATCTCGT237980.14436053372997398No Hit
CGGCGACTCTGGACGCGAGCATCTCGTATG237090.14382065275249822No Hit
CGCGACCTCAGATCAGACGTGGCGATCTCG236410.14340815942139318No Hit
CGGCGGCGGCGACTCTGGACGCGAGCATCT236070.14320191275584065No Hit
ACCCTGTAGAACCGAATTTGTATCTCGTAT234830.14244971903441375No Hit
CGGCGGCGGCGGCGACTCTGGACGCGATCT232000.1407330188476089No Hit
CGGCGGCGGCGACTCTGGACGCGATCTCGT228510.13861595748649616No Hit
TGTAAACATCCTCGACTGGAAGCATCTCGT218890.13278039006703926No Hit
TACCCTGTAGATCCGAATTTGTGAATCTCG214290.1299899940036815No Hit
CACCCGTAGAACCGACCTTGCGATCTCGTA212520.12891629812712863No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTAT207700.12599244833900158No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGT205000.12435460717137854No Hit
AAGCTGCCAGTTGAAGAACTGTAATCTCGT198480.12039952405548886No Hit
TTTGTTCGTTCGGCTCGCGTGAAATCTCGT198170.12021147562513214No Hit
TGTAAACATCCTTGACTGGAAGATCTCGTA196920.11945321582530666No Hit
TTTGGCAATGGTAGAACTCACACTATCTCG189230.1147884015367803No Hit
GGCGGCGGCGGCGACTCTGGACGCGATCTC178590.10833409412066583No Hit
GCGGCGGCGGCGACTCTGGACGCGATCTCG178560.10831589588547001No Hit
TGAGATGAAGCACTGTAGATCTCGTATGCC171380.10396045159527245No Hit
TTTGGCAATGGTAGAACTCACAATCTCGTA169930.1030808702274749No Hit
TGACCTATGAATTGACAGCCAGAATCTCGT165630.10047245651607527No Hit
TAGCTTATCAGACTGATGTTGACAATCTCG165040.10011455789055765No Hit
GCGGCGGCGGCGACTCTGGACGCGAGATCT164880.10001750063617998No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)