FastQCFastQC Report
Wed 8 May 2024
EGAF00001082983

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001082983
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14605032
Total Bases438.1 Mbp
Sequences flagged as poor quality0
Sequence length30
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCTTATCAGACTGATGTTGAATCTCGTA14153469.69081067401975No Hit
ATCTCGTATGCCGTCTTCTGCTTGAAAAAA12400578.49061474154935Illumina Paired End PCR Primer 2 (100% over 24bp)
TGAGATGAAGCACTGTAGCTCTATCTCGTA8496255.8173443235180855No Hit
TACCCTGTAGAACCGAATTTGTATCTCGTA6354764.351075711439729No Hit
TGACCTATGAATTGACAGCCAGATCTCGTA5711213.9104399086561403No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTA5487843.7574994700456665No Hit
TACCCTGTAGATCCGAATTTGTATCTCGTA4806943.2912902895385647No Hit
TGAGATGAAGCACTGTAGCTATCTCGTATG3574792.4476427028711747No Hit
TCAGTGCACTACAGAACTTTGATCTCGTAT2992532.048971888592918No Hit
TGTAACAGCAACTCCATGTGGAATCTCGTA2786531.9079246111888013No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTA2501421.7127110710883755No Hit
TTTGTTCGTTCGGCTCGCGTGAATCTCGTA2489711.7046932865330249No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGT1850631.2671180727300015No Hit
TAGCTTATCAGACTGATGTTGACATCTCGT1802301.2340267381817445No Hit
TGAGATGAAGCACTGTAGCTCATCTCGTAT1725871.1816954594827318No Hit
CTGACCTATGAATTGACAGCCATCTCGTAT1542331.056026443488792No Hit
TAGCTTATCAGACTGATGTTGATCTCGTAT1389130.9511310896134976No Hit
TACCCTGTAGAACCGAATTTGTGATCTCGT1102510.7548836592757894No Hit
TAATACTGCCGGGTAATGATGGAATCTCGT1029990.7052295400653693No Hit
CTGACCTATGAATTGACAGCCAATCTCGTA955210.6540280089766322No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTA628290.4301873491273419No Hit
TCAGTGCACTACAGAACTTTGAATCTCGTA608550.41667145953531626No Hit
TATTGCACTTGTCCCGGCCTGTATCTCGTA606590.41532945631341306No Hit
ACCCTGTAGATCCGAATTTGTATCTCGTAT605290.4144393521356201No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGT531610.36399098612040015No Hit
TGTAAACATCCTCGACTGGAAGATCTCGTA531010.36358016880757266No Hit
TACAGTACTGTGATAACTGAATCTCGTATG488650.33457646652194944No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC446110.30544951904247797No Hit
ACCCTGTAGATCCGAATTTGTGAATCTCGT443590.30372408632860237No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTA422650.2893865621109218No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTA422340.2891743064992942No Hit
TGAGATGAAGCACTGTAGCTCTTATCTCGT405010.27730853311379255No Hit
TAGCACCATCTGAAATCGGTTATCTCGTAT404560.2770004201291719No Hit
TGAGATGAAGCACTGTAGCTCAATCTCGTA402490.27558310039991696No Hit
GTCTACGGCCATACCACCCTGAACGCGCCC386930.2649292380872565No Hit
TAATACTGCCGGGTAATGATGGATCTCGTA386620.2647169824756289No Hit
ACCCTGTAGAACCGAATTTGTGATCTCGTA371690.2544944783414374No Hit
CACTAGATTGTGAGCTCCTGGAATCTCGTA367070.25133118503266544No Hit
TACCCTGTAGAACCGAATTTGATCTCGTAT364900.24984539575127257No Hit
GCGGCGGCGGCGACTCTGGACGCGAGCATC363150.24864717858885896No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTA344020.23554895326487474No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAA342180.234289113505537Illumina Paired End PCR Primer 2 (100% over 23bp)
CACCCGTAGAACCGACCTTGCGATCTCGTA325900.2231422704174835No Hit
TGTAACAGCAACTCCATGTGGATCTCGTAT318160.21784272708200844No Hit
TACCCTGTAGATCCGAATTTGATCTCGTAT290470.19888350809501823No Hit
GTACAGTACTGTGATAACTGAATCTCGTAT283230.19392631252023276No Hit
TGTAACAGCAACTCCATGTGGAAATCTCGT269500.18452544301169624No Hit
TACAGTACTGTGATAACTGAATATCTCGTA265730.18194414089609665No Hit
TGACCTATGAATTGACAGCCAGTATCTCGT265140.1815401705384829No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTAT264910.18138269056856568No Hit
TGAGATGAAGCACTGTAGCTCTAATCTCGT245620.16817491396116077No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTA238660.1634094331323615No Hit
ACCCTGTAGAACCGAATTTGTATCTCGTAT237750.16278636020790643No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGT235580.16130057092651354No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTA230430.15777438899141064No Hit
AACCCGTAGATCCGAACTTGTGAATCTCGT226400.15501506604025242No Hit
ACTGGACTTGGAGTCAGAAGGCATCTCGTA221560.15170113971677707No Hit
CTGACCTATGAATTGACAGCCAGATCTCGT217430.14887334721348094No Hit
TACCCTGTAGATCCGAATTTGTGAATCTCG217200.14871586724356373No Hit
TCAGTGCACTACAGAACTTTGTCATCTCGT215990.14788738566269488No Hit
TGAGATGAAGCACTGTAGCTAATCTCGTAT211580.1448678784134126No Hit
GTGAAATGTTTAGGACCACTAGATCTCGTA205620.14078709310599252No Hit
GCGGCGGCGGCGACTCTGGACGCGAGATCT199740.13676108344028276No Hit
GCGGCGACTCTGGACGCGAGCATCTCGTAT197150.13498772203991063No Hit
CGCGACCTCAGATCAGACGTGGATCTCGTA192990.13213938867097313No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGC188810.12927736139160803No Hit
TGAGGTAGTAGGTTGTGTGGTTATCTCGTA186820.12791481730406343No Hit
CGCGACCTCAGATCAGACGTGGCGATCTCG184590.12638794629138778No Hit
TGTAAACATCCTCGACTGGAAGCATCTCGT182950.1252650456363259No Hit
CACCCGTAGAACCGACCTTGCATCTCGTAT180550.1236217763850158No Hit
TAGCACCATTTGAAATCGGTTATCTCGTAT177210.12133489334360925No Hit
TGTAAACATCCTTGACTGGAAGATCTCGTA174240.11930134764511299No Hit
GCGGCGGCGGCGACTCTGGACGCGATCTCG173840.11902746943656133No Hit
ACCCTGTAGATCCGAATTTGTGTATCTCGT172640.11820583481090628No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTG170920.11702815851413403No Hit
ACCCTGTAGAACCGAATTTGTGTATCTCGT168860.11561768574009287No Hit
TCCAGCATCAGTGATTTTGTTGAATCTCGT162890.111530053477459No Hit
TAGCTTATCAGACTGATGTTGAAATCTCGT159110.10894190440664561No Hit
CTGACCTATGAATTGACAGCCATATCTCGT158780.10871595488459046No Hit
AAGCTGCCAGTTGAAGAACTGTAATCTCGT156810.10736710470747343No Hit
TAGCTTATCAGACTGATGTTGACAATCTCG153030.10477895563666002No Hit
TTTGGCAATGGTAGAACTCACACTATCTCG152530.10443660787597042No Hit
TACCCTGTAGAACCGAATTTGTGAATCTCG152340.10430651572690837No Hit
CTGACCTATGAATTGACAGCATCTCGTATG151830.10395732101100498No Hit
TGTAAACATCCTTGACTGGAAGCATCTCGT150390.10297135946021892No Hit
TGAGGTAGTAGGTTGTATAGATCTCGTATG149200.10215657178977768No Hit
CGGCGGCGGCGACTCTGGACGCGAGCATCT148750.10184845880515701No Hit
GGCGGCGGCGACTCTGGACGCGAGCATCTC148640.1017731422978053No Hit
TCGTACCGTGAGTAATAATGCGATCTCGTA148390.1016019684174605No Hit
TTTGTTCGTTCGGCTCGCGTGATCTCGTAT147090.10071186423966753No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)