FastQCFastQC Report
Wed 8 May 2024
EGAF00001082984

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001082984
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17873401
Total Bases536.2 Mbp
Sequences flagged as poor quality0
Sequence length30
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCTTATCAGACTGATGTTGAATCTCGTA17536439.811467890190569No Hit
ATCTCGTATGCCGTCTTCTGCTTGAAAAAA14645948.194265881462627Illumina Paired End PCR Primer 2 (100% over 24bp)
AAGCTGCCAGTTGAAGAACTGTATCTCGTA12489756.9878978264964795No Hit
TGACCTATGAATTGACAGCCAGATCTCGTA10805036.04531280868146No Hit
TTTGTTCGTTCGGCTCGCGTGAATCTCGTA8272584.628430817391721No Hit
TGTAACAGCAACTCCATGTGGAATCTCGTA7259224.061465414444627No Hit
TGAGATGAAGCACTGTAGCTCTATCTCGTA6902753.8620237972616405No Hit
TACCCTGTAGAACCGAATTTGTATCTCGTA4213522.3574248683840304No Hit
CTGACCTATGAATTGACAGCCATCTCGTAT3426671.917189683149838No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTA3377891.8898977312711778No Hit
TACCCTGTAGATCCGAATTTGTATCTCGTA3342751.8702372312913476No Hit
TCAGTGCACTACAGAACTTTGATCTCGTAT3202801.7919365206431614No Hit
TGAGATGAAGCACTGTAGCTATCTCGTATG2669451.4935322046430894No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGT2092791.1708963503924072No Hit
TAGCTTATCAGACTGATGTTGACATCTCGT1882451.0532130958176342No Hit
CTGACCTATGAATTGACAGCCAATCTCGTA1843851.0316167583326754No Hit
TAATACTGCCGGGTAATGATGGAATCTCGT1586190.8874584081675334No Hit
ATGACCTATGAATTGACAGACAATCTCGTA1542290.8628967704579559No Hit
TGAGATGAAGCACTGTAGCTCATCTCGTAT1457440.8154239923336359No Hit
TAGCTTATCAGACTGATGTTGATCTCGTAT1250460.6996206262031496No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTA1074810.6013461008344186No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGT918040.5136347581526314No Hit
TTTGTTCGTTCGGCTCGCGTGATCTCGTAT865420.4841943623376435No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC844390.4724282748425999No Hit
TGACCTATGAATTGACAGCCAGTATCTCGT812150.4543902976271836No Hit
TCAGTGCACTACAGAACTTTGAATCTCGTA742700.41553367487251025No Hit
TCCAGCATCAGTGATTTTGTTGAATCTCGT726340.4063804085187816No Hit
TACCCTGTAGAACCGAATTTGTGATCTCGT725510.40591603131379417No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTA702540.39306453203841846No Hit
ATGACCTATGAATTGACAGACATCTCGTAT683880.38262443728532697No Hit
TGTAACAGCAACTCCATGTGGATCTCGTAT681160.3811026228304283No Hit
TATTGCACTTGTCCCGGCCTGTATCTCGTA670100.37491465670131835No Hit
TGTAACAGCAACTCCATGTGGAAATCTCGT661360.3700247087837396No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTA543120.3038705392443218No Hit
TGTAAACATCCTCGACTGGAAGATCTCGTA494620.2767352447360186No Hit
ACCCTGTAGATCCGAATTTGTATCTCGTAT493790.27627086753103114No Hit
CTGACCTATGAATTGACAGCATCTCGTATG462260.25863012864759205No Hit
TAATACTGCCGGGTAATGATGGATCTCGTA454220.2541318241559063No Hit
CTGACCTATGAATTGACAGCCAGATCTCGT441610.2470766475837475No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAA423610.23700581663221232Illumina Paired End PCR Primer 2 (100% over 23bp)
TGAGGTAGTAGGTTGTATAGTATCTCGTAT407140.22779100631155758No Hit
TACCCTGTAGATCCGAATTTGATCTCGTAT377400.2111517556171878No Hit
AAGCTGCCAGTTGAAGAACTGTAATCTCGT375530.21010550817944498No Hit
TGTAACAGCAACTCCATGTGGACATCTCGT365960.20475118305687875No Hit
TACAGTACTGTGATAACTGAATCTCGTATG357870.2002249040347721No Hit
TGAGATGAAGCACTGTAGCTCAATCTCGTA354660.19842893918174836No Hit
TGAGATGAAGCACTGTAGCTCTTATCTCGT330940.18515782195005864No Hit
ACCCTGTAGATCCGAATTTGTGAATCTCGT327450.18320519972667765No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTA326650.18275760723994275No Hit
TTTGTTCGTTCGGCTCGCGTGAAATCTCGT321580.17992098985526034No Hit
TACCCTGTAGAACCGAATTTGATCTCGTAT319780.17891390676010682No Hit
AAGCTGCCAGTTGAAGAACTGTTATCTCGT314240.17581432878946765No Hit
CACCCGTAGAACCGACCTTGCGATCTCGTA305740.1710586586179094No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGC290880.16274462817680865No Hit
TTTGGCAATGGTAGAACTCACAATCTCGTA289760.1621179986953798No Hit
TTTGGCAATGGTAGAACTCACACTATCTCG289750.16211240378929562No Hit
CACTAGATTGTGAGCTCCTGGAATCTCGTA284840.1593653049019602No Hit
GTCTACGGCCATACCACCCTGAACGCGCCC280640.15701544434660197No Hit
TAGCACCATCTGAAATCGGTTATCTCGTAT272050.152209420020286No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGT269800.1509505661513441No Hit
TCAGTGCACTACAGAACTTTGTCATCTCGT266240.14895877958537382No Hit
TCGTACCGTGAGTAATAATGCGATCTCGTA264430.1479461015841361No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTA260050.14549553271926255No Hit
CTGACCTATGAATTGACAGATCTCGTATGC252460.1412489990013652No Hit
TGAGATGAAGCACTGTAGCTCTAATCTCGT252130.14106436710058706No Hit
ACCCTGTAGAACCGAATTTGTGATCTCGTA249750.13973277945255075No Hit
CTGACCTATGAATTGACAGCCATATCTCGT241030.13485402134714036No Hit
TAGCTTATCAGACTGATGTTGACAATCTCG233590.13069141122050582No Hit
TACAGTACTGTGATAACTGAATATCTCGTA231200.12935422866638532No Hit
TCCAGCATCAGTGATTTTGTTGATCTCGTA225700.1262770303200829No Hit
TGAGGTAGTAGGTTGTATAGATCTCGTATG225130.1259581206732843No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTA225080.12593014614286335No Hit
GTACAGTACTGTGATAACTGAATCTCGTAT220100.12314388291293861No Hit
TGAGATGAAGCACTGTAGCTAATCTCGTAT208870.1168608033803975No Hit
TCCCTGGTCTAGTGGTTAGGATTCGGCGCA208280.11653070392143051No Hit
TGACCTATGAATTGACAGCCAGAATCTCGT207190.11592085915825422No Hit
ACTGGACTTGGAGTCAGAAGGCATCTCGTA206790.11569706291488675No Hit
TGAGGTAGTAGGTTGTGTGGTTATCTCGTA198280.1109357978372443No Hit
TAGCTTATCAGACTGATGTTGAAATCTCGT198110.11084068443381312No Hit
CGCGACCTCAGATCAGACGTGGATCTCGTA194470.10880413861916935No Hit
GTGAAATGTTTAGGACCACTAGATCTCGTA191270.10701376867222975No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCG184730.10335470009317196No Hit
TTTGTTCGTTCGGCTCGCGTGTATCTCGTA183400.10261057758397521No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCA179030.10016560362518584No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)