FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001082985

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001082985
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1631570
Sequences flagged as poor quality0
Sequence length30
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTATGCCGTCTTCTGCTTGAAAAAA21873013.40610577541877Illumina Single End Adapter 1 (95% over 24bp)
TAGCTTATCAGACTGATGTTGAATCTCGTA940575.764815484472011No Hit
TTTGTTCGTTCGGCTCGCGTGAATCTCGTA699564.287649319367235No Hit
TGACCTATGAATTGACAGCCAGATCTCGTA560853.4374865926684115No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTA460602.8230477392940543No Hit
TGAGATGAAGCACTGTAGCTCTATCTCGTA400602.4553037871498007No Hit
TCAGTGCACTACAGAACTTTGATCTCGTAT325961.997830310682349No Hit
TGAGATGAAGCACTGTAGCTATCTCGTATG287761.7636999944838405No Hit
TACCCTGTAGAACCGAATTTGTATCTCGTA284111.7413289040617321No Hit
CTGACCTATGAATTGACAGCCATCTCGTAT229861.408827080664636No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC188161.1532450339243796No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTA182501.1185545211054384No Hit
TAGCTTATCAGACTGATGTTGACATCTCGT163541.0023474322278543No Hit
TACCCTGTAGATCCGAATTTGTATCTCGTA156490.9591375178509044No Hit
TGTAACAGCAACTCCATGTGGAATCTCGTA154230.945285828986804No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGT125470.7690138945923252No Hit
TAGCTTATCAGACTGATGTTGATCTCGTAT124100.760617074351698No Hit
CTGACCTATGAATTGACAGCCAATCTCGTA113410.6950973602113302No Hit
TGAGATGAAGCACTGTAGCTCATCTCGTAT109710.6724198164957679No Hit
TATTGCACTTGTCCCGGCCTGTATCTCGTA106290.6514584112235454No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAA83480.5116544187500383Illumina Single End Adapter 1 (95% over 23bp)
CACCCGTAGAACCGACCTTGCGATCTCGTA72560.4447250194597841No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGT71160.43614432724308494No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGC70500.4320991437694981No Hit
GTGAAATGTTTAGGACCACTAGATCTCGTA67810.41561195658169736No Hit
TACCCTGTAGAACCGAATTTGTGATCTCGT65810.4033538248435556No Hit
TCAGTGCACTACAGAACTTTGAATCTCGTA62690.3842311393320544No Hit
TTTGTTCGTTCGGCTCGCGTGATCTCGTAT60140.3686020213659236No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTG56840.3483761039979897No Hit
TGTAAACATCCTCGACTGGAAGATCTCGTA54710.3353211936968687No Hit
CGGCGACTCTGGACGCGAGCCGGGATCTCG54150.33188891681018895No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTA49330.3023468193212672No Hit
TAGCACCATCTGAAATCGGTTATCTCGTAT45340.2778918465036744No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCAT45290.27758539321022085No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCC37650.23075932997051918No Hit
CACTAGATTGTGAGCTCCTGGAATCTCGTA36970.22659156517955098No Hit
GCGGCGGCGGCGACTCTGGACGCGAGCATC36950.22646898386216957No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTA36800.22554962398180894No Hit
CGCGACCTCAGATCAGACGTGGATCTCGTA36100.2212592778734593No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTA36010.2207076619452429No Hit
TAATACTGCCGGGTAATGATGGAATCTCGT34360.21059470326127597No Hit
CAACACCAGTCGATGGGCTGTCATCTCGTA33580.20581403188340064No Hit
GCGGCGACTCTGGACGCGAGCATCTCGTAT33160.20323982421839087No Hit
TGAAATGTTTAGGACCACTAGATCTCGTAT31210.19128814577370265No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTG30270.185526823856776No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCG29730.18221712828747771No Hit
CACCCGTAGAACCGACCTTGCATCTCGTAT29540.18105260577235424No Hit
TACCCTGTAGAACCGAATTTGATCTCGTAT29320.17970421128115865No Hit
CGGCGACTCTGGACGCGAGCATCTCGTATG28670.17572031846626257No Hit
GTCTACGGCCATACCACCCTGAACGCGCCC28450.17437192397506696No Hit
TTTGGCAATGGTAGAACTCACAATCTCGTA28120.17234933223827356No Hit
TTTGGCAATGGTAGAACTCACACTATCTCG26830.1644428372671721No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGT26580.16291057079990437No Hit
GCGGCGACTCTGGACGCGAGCCGGGATCTC25590.1568427955895242No Hit
TGTAACAGCAACTCCATGTGGATCTCGTAT25380.1555556917570193No Hit
GCGGCGGCGGCGACTCTGGACGCGATCTCG24970.15304277475070024No Hit
ACCCTGTAGAACCGAATTTGTGATCTCGTA24950.15292019343331884No Hit
TCGTACCGTGAGTAATAATGCGATCTCGTA24700.1513879269660511No Hit
ACCCTGTAGATCCGAATTTGTATCTCGTAT24010.14715887151639218No Hit
CGGCGACTCTGGACGCGAGCCATCTCGTAT23310.14286852540804257No Hit
CGGCGGCGGCGACTCTGGACGCGAGCATCT22970.14078464301255847No Hit
CTGACCTATGAATTGACAGCATCTCGTATG22740.13937495786267215No Hit
CTGTGCGTGTGACAGCGGCTGAATCTCGTA22340.1369233315150438No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGA21970.13465557714348755No Hit
TGAGGTAGTAGGTTGTGTGGTTATCTCGTA21770.13342976396967338No Hit
TCAGTGCACTACAGAACTTTGTCATCTCGT21490.13171362552633353No Hit
TGAGATGAAGCACTGTAGATCTCGTATGCC21420.13128459091549857No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCATC21400.13116200959811714No Hit
TGGCTCAGTTCAGCAGGAACAGTATCTCGT20120.1233168052857064No Hit
CGCGACCTCAGATCAGACGTGGCGATCTCG20070.12301035199225287No Hit
CGCGACCTCAGATCAGACGTGGCATCTCGT20010.1226426080401086No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTA19770.1211716322315316No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTA19540.1197619470816453No Hit
GCGGCGGCGGCGACTCTGGACGCGAGATCT19270.11810709929699614No Hit
CTGACCTATGAATTGACAGCCAGATCTCGT18890.11577805426674921No Hit
CGGCGGCGGCGGCGACTCTGGACGCGATCT18890.11577805426674921No Hit
GCGGCGACTCTGGACGCGAGCCATCTCGTA18310.11222319606268807No Hit
TGACCTATGAATTGACAGCCAGTATCTCGT17870.10952640708029689No Hit
GGGCGCGCGCCGCGGCTGGACGAGATCTCG17670.10830059390648271No Hit
GGTTCCATGGTGTAATGGTTAGCACTCTGG17530.10744252468481279No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGC17020.10431670109158662No Hit
CGGCGACTCTGGACGCGAGCCGGGCATCTC16890.10351992252860742No Hit
TACAGTACTGTGATAACTGAATCTCGTATG16850.10327475989384458No Hit
CATCTTACCGGACAGTGCTGGAATCTCGTA16760.1027231439656282No Hit
CGGCGACTCTGGACGCGAGCCGATCTCGTA16740.10260056264824678No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCA16720.10247798133086537No Hit
TGAGATGAAGCACTGTAGCTCTTATCTCGT16600.10174249342657686No Hit
GCGGCGGCGGCGACTCTGGACGATCTCGTA16510.10119087749836048No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)