FastQCFastQC Report
Wed 8 May 2024
EGAF00001082986

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001082986
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5446585
Total Bases163.3 Mbp
Sequences flagged as poor quality0
Sequence length30
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCTTATCAGACTGATGTTGAATCTCGTA62052211.392863601688031No Hit
ATCTCGTATGCCGTCTTCTGCTTGAAAAAA5441049.989819308796246Illumina Paired End PCR Primer 2 (100% over 24bp)
AAGCTGCCAGTTGAAGAACTGTATCTCGTA2122883.897634940058771No Hit
TACCCTGTAGATCCGAATTTGTATCTCGTA1737403.189888710081638No Hit
TCAGTGCACTACAGAACTTTGATCTCGTAT1232252.262426823413203No Hit
TAGCTTATCAGACTGATGTTGACATCTCGT1089542.00040943086356No Hit
CACCCGTAGAACCGACCTTGCGATCTCGTA925911.6999826496786516No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGT880351.616333904639329No Hit
TGACCTATGAATTGACAGCCAGATCTCGTA819311.5042636808201837No Hit
TACCCTGTAGAACCGAATTTGTATCTCGTA783581.4386629420086163No Hit
TGAGATGAAGCACTGTAGCTATCTCGTATG761271.3977014955242597No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTA712361.3079021074673396No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGT674921.2391617866975362No Hit
TAGCTTATCAGACTGATGTTGATCTCGTAT580611.0660074156558652No Hit
TGAGATGAAGCACTGTAGCTCTATCTCGTA560041.0282406315149768No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTA435080.7988124668943934No Hit
TATTGCACTTGTCCCGGCCTGTATCTCGTA398540.7317245576815564No Hit
TTTGGCAATGGTAGAACTCACACTATCTCG384190.7053777734121472No Hit
TTTGTTCGTTCGGCTCGCGTGAATCTCGTA336340.6175245589667654No Hit
TAATACTGCCGGGTAATGATGGAATCTCGT308580.5665568424985564No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTA290360.5331046885341916No Hit
TTTGGCAATGGTAGAACTCACAATCTCGTA280020.5141203157574884No Hit
CTGACCTATGAATTGACAGCCATCTCGTAT271020.49759620018782413No Hit
ACCCTGTAGATCCGAATTTGTATCTCGTAT257840.4733975509424713No Hit
TGTAACAGCAACTCCATGTGGAATCTCGTA235990.4332806703650085No Hit
CACCCGTAGAACCGACCTTGCATCTCGTAT221630.4069155259671886No Hit
TTTGGCAATGGTAGAACTCACACTGATCTC207380.3807523429818868No Hit
TCAGTGCACTACAGAACTTTGAATCTCGTA205540.377374079354311No Hit
CAACACCAGTCGATGGGCTGTCATCTCGTA203270.37320633020507343No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAA197690.3629613785518816Illumina Paired End PCR Primer 2 (100% over 23bp)
TATGGCACTGGTAGAATTCACTGATCTCGT173660.3188419899808779No Hit
ATGGCACTGGTAGAATTCACTGATCTCGTA166230.3052004145716995No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTA162570.29848060757336936No Hit
CTGACCTATGAATTGACAGCCAATCTCGTA155610.2857019581994956No Hit
TAGCACCATCTGAAATCGGTTATCTCGTAT153650.282103373030991No Hit
TACCCTGTAGATCCGAATTTGATCTCGTAT146230.2684801577502233No Hit
TACCCTGTAGAACCGAATTTGTGATCTCGT136450.25052395216452145No Hit
TGAGATGAAGCACTGTAGCTCATCTCGTAT119840.22002777887428546No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGT112610.2067534060333218No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC105100.19296494959685748No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTA99180.18209575357770053No Hit
GGCCGCGTGGCCTAATGGAATCTCGTATGC92310.16948234535952345No Hit
CAAAGTGCTGTTCGTGCAGGTAGAATCTCG89000.16340514285556912No Hit
AATATCAACGTATTGTAGGACTATCTCGTA88030.16162421039972755No Hit
TTTGGCAATGGTAGAACTCACACTGGATCT87130.1599717988427611No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTAT86430.15868658985400944No Hit
ACCCTGTAGATCCGAATTTGTGAATCTCGT85580.15712597893909672No Hit
AGCAGCATTGTACAGGGCTATGATCTCGTA84780.15565716866623766No Hit
ACCGGGTGCTGTAGGCTTTATCTCGTATGC84070.15435359954907524No Hit
GTGAAATGTTTAGGACCACTAGATCTCGTA83200.15275626837734105No Hit
AAGCTGCCAGTTGAAGAACTGTAATCTCGT81870.15031437129871286No Hit
TAGCTTATCAGACTGATGTTGACTATCTCG81780.15014913014301623No Hit
TATGGCACTGGTAGAATTCACTATCTCGTA80590.1479642748621384No Hit
TGTAAACATCCTCGACTGGAAGATCTCGTA78290.14374144532766864No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTA76490.14043662221373576No Hit
TAGCTTATCAGACTGATGTTGACAATCTCG76010.13955533605002035No Hit
TACCCTGTAGAACCGAATTTGATCTCGTAT74820.1373704807691425No Hit
TACAGTACTGTGATAACTGAATCTCGTATG72820.13369845508699488No Hit
TAGCTTATCAGACTGATGTTGAAATCTCGT72210.13257848725393986No Hit
CACTAGATTGTGAGCTCCTGGAATCTCGTA71110.13055887312875866No Hit
CTAGCACCATCTGAAATCGGTTATCTCGTA69400.12741929117052245No Hit
TGAGATGAAGCACTGTAGATCTCGTATGCC65720.12066276391537083No Hit
TCGTACCGTGAGTAATAATGCGATCTCGTA65150.11961623659595874No Hit
TCAGTGCACTACAGAACTTTGTCATCTCGT64790.11895527197317218No Hit
GTAGTGTTTCCTACTTTATGGATCTCGTAT64610.1186247896617789No Hit
TGAGGTAGTAGGTTGTGTGGTTATCTCGTA62870.11543012731831045No Hit
GTACAGTACTGTGATAACTGAATCTCGTAT61110.11219874471802055No Hit
CTTTGACATGATGTACAGAAAAAAGTATCT60510.11109713701337627No Hit
TTTGGCAATGGTAGAACTCACACATCTCGT57770.10606646182883403No Hit
TCAGTGCACTACAGAACTTTGTTATCTCGT57470.1055156579765119No Hit
TAGCACCATCTGAAATCGGTTAATCTCGTA57330.10525861617876155No Hit
TAATACTGCCGGGTAATGATGGATCTCGTA55500.10189871267959648No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)