FastQCFastQC Report
Wed 8 May 2024
EGAF00001082987

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001082987
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5854135
Total Bases175.6 Mbp
Sequences flagged as poor quality0
Sequence length30
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCTTATCAGACTGATGTTGAATCTCGTA68070011.627678555414251No Hit
ATCTCGTATGCCGTCTTCTGCTTGAAAAAA5274519.00988788266755Illumina Paired End PCR Primer 2 (100% over 24bp)
TACCCTGTAGAACCGAATTTGTATCTCGTA3179335.430913362947728No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTA2318853.9610463373325007No Hit
TTTGTTCGTTCGGCTCGCGTGAATCTCGTA2144083.6625052206688093No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTA1977943.3787058207574647No Hit
TAGCTTATCAGACTGATGTTGACATCTCGT1362122.3267656109741233No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGT1112821.9009127736206972No Hit
TATTGCACTTGTCCCGGCCTGTATCTCGTA867941.482610155044255No Hit
TGACCTATGAATTGACAGCCAGATCTCGTA866411.479996617775299No Hit
TGAGATGAAGCACTGTAGCTATCTCGTATG794721.3575361688789205No Hit
TACCCTGTAGAACCGAATTTGTGATCTCGT771091.317171537725044No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGT700671.1968804955813284No Hit
GTGAAATGTTTAGGACCACTAGATCTCGTA592731.0124980035479194No Hit
TAGCTTATCAGACTGATGTTGATCTCGTAT585431.0000281852058417No Hit
TGAGATGAAGCACTGTAGCTCTATCTCGTA566710.9680507880327325No Hit
TACCCTGTAGATCCGAATTTGTATCTCGTA536720.9168220411726071No Hit
TGTAACAGCAACTCCATGTGGAATCTCGTA448550.7662105503204145No Hit
CACCCGTAGAACCGACCTTGCGATCTCGTA439670.7510417849947089No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTA428940.7327128602261478No Hit
TCAGTGCACTACAGAACTTTGTCATCTCGT362170.6186567272534712No Hit
TAATACTGCCGGGTAATGATGGAATCTCGT330050.5637895265483287No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTA327760.5598777616163617No Hit
TTTGGCAATGGTAGAACTCACACTATCTCG325530.556068488342001No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC308070.5262434159786202No Hit
CTGACCTATGAATTGACAGCCATCTCGTAT298700.5102376354491313No Hit
TGAAATGTTTAGGACCACTAGATCTCGTAT283760.4847172127052075No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGT277410.4738701789418932No Hit
TCAGTGCACTACAGAACTTTGATCTCGTAT276780.47279401653702896No Hit
ACCCTGTAGAACCGAATTTGTGATCTCGTA238790.407899715329421No Hit
CAACACCAGTCGATGGGCTGTCATCTCGTA204300.34898409414883663No Hit
CTGACCTATGAATTGACAGCCAATCTCGTA183240.31300952232908874No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAA180690.3086536268808287Illumina Paired End PCR Primer 2 (100% over 23bp)
GTGAAATGTTTAGGACCACTAGAATCTCGT174340.2978065931175144No Hit
TTTGGCAATGGTAGAACTCACAATCTCGTA172870.29529554750616444No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTA148400.25349603314580205No Hit
TACCCTGTAGAACCGAATTTGATCTCGTAT139960.23907887330920793No Hit
TCGTACCGTGAGTAATAATGCGATCTCGTA135540.2315286545322238No Hit
CACCCGTAGAACCGACCTTGCATCTCGTAT131960.22541331896172534No Hit
CTTCTCACTACTGCACTTGACTAGATCTCG118040.20163525439710564No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTA116920.19972207678845807No Hit
TTTGGCAATGGTAGAACTCACACTGATCTC114360.19534909939726364No Hit
TAGCTTATCAGACTGATGTTGACTATCTCG113980.1946999855657582No Hit
TTTGGCAATGGTAGAACTCACACATCTCGT111720.1908394664625944No Hit
TGAGATGAAGCACTGTAGCTCATCTCGTAT111630.19068572897618521No Hit
TATGGCACTGGTAGAATTCACTGATCTCGT107890.1842970823187371No Hit
ACCCTGTAGAACCGAATTTGTATCTCGTAT104050.17773761623194545No Hit
TGTAAACATCCTCGACTGGAAGATCTCGTA102470.17503866924831765No Hit
CAAAGTGCTTACAGTGCAGGTAGATCTCGT95690.16345711193882614No Hit
TTCTCACTACTGCACTTGACTAGATCTCGT95650.16338878416708874No Hit
ACCCTGTAGAACCGAATTTGTGTATCTCGT94970.1622272120475527No Hit
TACAGTACTGTGATAACTGAATCTCGTATG93820.16026278861010207No Hit
TAGCACCATCTGAAATCGGTTATCTCGTAT91380.1560947945341199No Hit
TACAGTACTGTGATAACTGAATATCTCGTA90650.1548478126999121No Hit
TACCCTGTAGAACCGAATTTGTGAATCTCG89210.15238801291736526No Hit
TCAGTGCACTACAGAACTTTGAATCTCGTA87830.1500307047924245No Hit
TAGCTTATCAGACTGATGTTGAAATCTCGT87100.1487837229582167No Hit
AACCCGTAGATCCGAACTTGTGAATCTCGT86710.14811752718377694No Hit
CATCTTACCGGACAGTGCTGGAATCTCGTA86570.14787837998269598No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGC86230.147297593922928No Hit
AGTCTGTGATGAATTGCTTTGACTTCTGAC84920.1450598593985277No Hit
CCCCCCACTGCTAAATTTGACTGGATCTCG83520.14266838738771825No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCC83300.1422925846431625No Hit
TAGCTTATCAGACTGATGTTGACAATCTCG82080.14020858760517138No Hit
TGAAATGTTTAGGACCACTAGTATCTCGTA81860.13983278486061562No Hit
CACTAGATTGTGAGCTCCTGGAATCTCGTA80890.13817583639598335No Hit
TTTGTTCGTTCGGCTCGCGTGAAATCTCGT79960.1365872157030885No Hit
TCAGTGCACTACAGAACTTTGTCTATCTCG79920.13651888793135109No Hit
CAAAGTGCTGTTCGTGCAGGTAGAATCTCG79170.1352377422112746No Hit
TAATACTGCCGGGTAATGATGGATCTCGTA78940.13484485752378447No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTAT77710.13274377854285904No Hit
AGCAGCATTGTACAGGGCTATGATCTCGTA76770.1311380759070298No Hit
TTTGTTCGTTCGGCTCGCGTGATCTCGTAT76430.1305572898472618No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTA75970.12977152047228155No Hit
TGAGGTAGTAGGTTGTGTGGTTATCTCGTA75380.1287636858391547No Hit
GTCTACGGCCATACCACCCTGAACGCGCCC75170.1284049650375333No Hit
TGGCTCAGTTCAGCAGGAACAGTATCTCGT75090.12826830949405846No Hit
AAGCTGCCAGTTGAAGAACTGTAATCTCGT75070.12823414560818977No Hit
TCAGTGCACTACAGAACTTTGTTATCTCGT72930.12457860982023818No Hit
TGAAATGTTTAGGACCACTAGAATCTCGTA68680.11731878407313803No Hit
TAAAGTGCTTATAGTGCAGGTAGATCTCGT68440.11690881744271357No Hit
ATGGCACTGGTAGAATTCACTGATCTCGTA67760.11574724532317755No Hit
AGCAGCATTGTACAGGGCTCTGAATCTCGT66330.11330452748356504No Hit
GTACAGTACTGTGATAACTGAATCTCGTAT65750.11231377479337255No Hit
TGAGATGAAGCACTGTAGATCTCGTATGCC65590.1120404637064229No Hit
ACCCTGTAGATCCGAATTTGTATCTCGTAT61570.1051735226468129No Hit
TATTGCACTTGTCCCGGCCTGTAAATCTCG61330.10476355601638841No Hit
TAATACTGCCTGGTAATGATGACATCTCGT60670.1036361477827211No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTG60300.10300411589415004No Hit
TGGCTCAGTTCAGCAGGAACAGATCTCGTA60010.10250873954905379No Hit
TACCCTGTAGAACCGAATTTGTGTATCTCG59570.10175713405994224No Hit
ACCCTGTAGAACCGAATTTGTGAATCTCGT59080.10092011885615895No Hit
TCCCTGGTCTAGTGGTTAGGATTCGGCGCA58980.10074929942681539No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)