FastQCFastQC Report
Wed 8 May 2024
EGAF00001082988

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001082988
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14824641
Total Bases444.7 Mbp
Sequences flagged as poor quality0
Sequence length30
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTATGCCGTCTTCTGCTTGAAAAAA12480638.418841306173958Illumina Paired End PCR Primer 2 (100% over 24bp)
TACCCTGTAGAACCGAATTTGTATCTCGTA11823937.975862619539995No Hit
TAGCTTATCAGACTGATGTTGAATCTCGTA9447696.372963770252514No Hit
TGAGATGAAGCACTGTAGCTCTATCTCGTA7321824.93895265322108No Hit
TGACCTATGAATTGACAGCCAGATCTCGTA7197064.854795471944312No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTA5533283.732488361775506No Hit
TACCCTGTAGATCCGAATTTGTATCTCGTA4231422.8543153254099036No Hit
TCAGTGCACTACAGAACTTTGATCTCGTAT4174892.8161828674299767No Hit
TTTGTTCGTTCGGCTCGCGTGAATCTCGTA3954802.6677205876351406No Hit
TGTAACAGCAACTCCATGTGGAATCTCGTA2939041.9825370476087751No Hit
TGAGATGAAGCACTGTAGCTATCTCGTATG2931181.9772350642420278No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTA2712211.8295282833493236No Hit
TACCCTGTAGAACCGAATTTGTGATCTCGT2243831.5135813406881151No Hit
CTGACCTATGAATTGACAGCCATCTCGTAT1887411.273157306136452No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGT1802841.2161103934995796No Hit
TGAGATGAAGCACTGTAGCTCATCTCGTAT1478970.9976430457911257No Hit
TAGCTTATCAGACTGATGTTGACATCTCGT1268230.8554878327239088No Hit
CTGACCTATGAATTGACAGCCAATCTCGTA1150200.7758703903858448No Hit
TAATACTGCCGGGTAATGATGGAATCTCGT1117250.7536438825061599No Hit
TCAGTGCACTACAGAACTTTGAATCTCGTA1011850.6825460393948157No Hit
TAGCTTATCAGACTGATGTTGATCTCGTAT723110.48777572421483933No Hit
ACCCTGTAGAACCGAATTTGTGATCTCGTA705160.4756675052029927No Hit
TAGCACCATCTGAAATCGGTTATCTCGTAT639310.4312482170731824No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGT605870.4086911784238148No Hit
TATTGCACTTGTCCCGGCCTGTATCTCGTA605470.40842135738733915No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTA580900.39184760022181986No Hit
TGTAAACATCCTCGACTGGAAGATCTCGTA576250.38871093067278994No Hit
TACCCTGTAGAACCGAATTTGATCTCGTAT563580.3801643493424225No Hit
ACCCTGTAGATCCGAATTTGTATCTCGTAT558540.3767646042828289No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTA511710.3451753064374375No Hit
TACAGTACTGTGATAACTGAATCTCGTATG453470.30588936352657714No Hit
ACCCTGTAGAACCGAATTTGTATCTCGTAT443610.29923827497745137No Hit
TTTGGCAATGGTAGAACTCACACTATCTCG400120.2699019827866321No Hit
ACCCTGTAGATCCGAATTTGTGAATCTCGT392420.26470792783447505No Hit
CACTAGATTGTGAGCTCCTGGAATCTCGTA386460.26068759439098727No Hit
TAATACTGCCGGGTAATGATGGATCTCGTA380560.2567077341029708No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAA372390.2511966394329549Illumina Paired End PCR Primer 2 (100% over 23bp)
TGTAAACATCCCCGACTGGAAGATCTCGTA362410.24446460457288646No Hit
TGAGATGAAGCACTGTAGCTCAATCTCGTA359440.24246118337705444No Hit
TGAGATGAAGCACTGTAGCTCTTATCTCGT357830.24137515370523982No Hit
TGACCTATGAATTGACAGCCAGTATCTCGT357730.24130769844612088No Hit
TACCCTGTAGAACCGAATTTGTGAATCTCG357070.24086249373593602No Hit
TGTAACAGCAACTCCATGTGGATCTCGTAT333800.2251656549389628No Hit
GTCTACGGCCATACCACCCTGAACGCGCCC333400.22489583390248707No Hit
TGTAACAGCAACTCCATGTGGAAATCTCGT332910.22456530313280437No Hit
ACCCTGTAGAACCGAATTTGTGTATCTCGT331480.22360069292740378No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTA327000.22057869731887605No Hit
GTGAAATGTTTAGGACCACTAGATCTCGTA323220.21802888852418081No Hit
TACAGTACTGTGATAACTGAATATCTCGTA299080.20174518897287294No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC294340.198547809690636No Hit
ACCCTGTAGAACCGAATTTGTGAATCTCGT288010.19427789178840824No Hit
TAGCACCATTTGAAATCGGTTATCTCGTAT280410.18915129209537013No Hit
TGAGATGAAGCACTGTAGCTCTAATCTCGT277890.1874514195655733No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGT277590.18724905378821655No Hit
ACTGGACTTGGAGTCAGAAGGCATCTCGTA269950.18209547199153087No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTA269410.1817312135922887No Hit
TTTGGCAATGGTAGAACTCACAATCTCGTA269170.18156932097040326No Hit
CACCCGTAGAACCGACCTTGCGATCTCGTA267920.18072613023141673No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTA267590.1805035278763243No Hit
CTGACCTATGAATTGACAGCCAGATCTCGT257720.17384569380128667No Hit
CGCGACCTCAGATCAGACGTGGATCTCGTA250710.1691170801370502No Hit
AACCCGTAGATCCGAACTTGTGAATCTCGT250590.1690361338261075No Hit
GTACAGTACTGTGATAACTGAATCTCGTAT233350.15740684715400527No Hit
TTTGTTCGTTCGGCTCGCGTGATCTCGTAT233110.15724495453211987No Hit
TCAGTGCACTACAGAACTTTGTCATCTCGT232650.1569346603401728No Hit
TACCCTGTAGATCCGAATTTGATCTCGTAT221350.1493122160597346No Hit
TACCCTGTAGAACCGAATTTGTGTATCTCG220890.14900192186778755No Hit
TGAGATGAAGCACTGTAGCTAATCTCGTAT212620.14342337193865268No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTAT202860.13683973864864588No Hit
TACCCTGTAGATCCGAATTTGTGAATCTCG200620.13532874084438198No Hit
TAGCACCATTTGAAATCGGTATCTCGTATG199070.1342831843280387No Hit
TCGTACCGTGAGTAATAATGCGATCTCGTA192050.12954782513789034No Hit
CTGACCTATGAATTGACAGCATCTCGTATG191030.12885978149487734No Hit
CTGACCTATGAATTGACAGCCATATCTCGT190200.1282999028441903No Hit
TAACACTGTCTGGTAAAGATGGCATCTCGT189750.12799635417815514No Hit
TAGCACCATCTGAAATCGGTTAATCTCGTA186960.1261143524487372No Hit
AAGCTGCCAGTTGAAGAACTGTAATCTCGT180600.12182419796877375No Hit
CACCCGTAGAACCGACCTTGCATCTCGTAT178670.12052231146777856No Hit
CGCGACCTCAGATCAGACGTGGCGATCTCG177860.11997592386891527No Hit
TGTAAACATCCTTGACTGGAAGCATCTCGT177410.11967237520288013No Hit
TCCAGCATCAGTGATTTTGTTGAATCTCGT177330.11961841099558498No Hit
TGGCTCAGTTCAGCAGGAACAGTATCTCGT172600.11642777723925996No Hit
TTTGTTCGTTCGGCTCGCGTGAAATCTCGT163250.11012071051164073No Hit
TGTAAACATCCTTGACTGGAAGATCTCGTA156030.10525044080325453No Hit
TGAAATGTTTAGGACCACTAGATCTCGTAT154310.10409021034640906No Hit
TAATACTGCCTGGTAATGATGACATCTCGT152590.10292997988956361No Hit
TAATACTGCCTGGTAATGATGAATCTCGTA151040.10188442337322029No Hit
TGTAAACATCCTCGACTGGAAGCATCTCGT150220.10133129024844514No Hit
TGAGATGAAGCACTGTAGATCTCGTATGCC149880.1011019423674408No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)