FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001104915

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001104915
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104838
Sequences flagged as poor quality0
Sequence length251
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA53765.1279116350941445No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA40463.859287662870333No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA27142.588755985425132No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA26572.53438638661554No Hit
CCTGTTTGATACCCGCACTTTCGAGCATCAACGTCAGTTACGGTCCAGCA25012.3855853793471833No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTCAGTTACGGCTTAGTG15611.4889639252942635No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCA14961.4269635055991148No Hit
CCTGTTTGATACCCGCACTTTCGAGCCTCAACGTCAGTTACGGTCCAGCA14151.3497014441328525No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAACGTCAGTTACAGCTTAGCA12651.2066235525286633No Hit
CCTGTTTGCTACCCACGCTTTCGCGCCTCAGCGTCAGTTAAAGCCCAGCA8880.8470211182968007No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA8100.7726206146626223No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGCA7010.6686506800969114No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA6920.6600660066006601No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAACGTCAGTGGCGGTTTTGTA6570.6266811652263492No Hit
CCTGTTCGATCCCCGCGCTTTCGTGCCTCAGCGTCCGTATGCCGCTTGCA6200.5913886186306492No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAACGTCAGTGGCGGTTTTGCA6180.58948091340926No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGTA5780.5513268089814761No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA5710.544649840706614No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA5640.5379728724317518No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA5430.5179419676071654No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTA5400.5150804097750815No Hit
CCTGTTTGATACCCGCACTTTCGAGCCTCAGCGTCAGTTATGCCCCGGCA4980.4750186001259086No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAGCAGTCCAGTA4670.44544916919437605No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCA4600.43877220091951397No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGAA4290.40920276998798144No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCA4120.3929872756061733No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAGGCCCAGTA4100.3910795703847841No Hit
CCTGTTTGATACCCGCACCTTCGAGCCTCAGCGTCAGTCGGCCACCGGGC4040.3853564547206166No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTG3960.3777256338350598No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAACAGTCCAGCA3920.3739102233922814No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGTA3880.370094812949503No Hit
CCTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCTGTTTCACCCTAGCA3640.34720235029283275No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTATCGTCCAGCA3380.32240218241477325No Hit
CCTGTTTGCTACCCGCACTTTCGAGCATCAACGTCAGTTACGGTCCAGCA3340.31858677197199486No Hit
CCTGTTTGCTACCCACACTTTCGCGCCTCAGCGTCAGTTGGTGCCCAGTA3260.31095595108643814No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG3170.3023712775901867No Hit
CCGGTTCGCGCCCCTAGCTTTCGTCCCTCACCGTCAGAATCGTTCCAGTC3010.28710963581907323No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCCGATATAGATTTGTA2960.28234037276560026No Hit
CCCTTTCGCTCCCCTGGCCTTCGTGCCTCAGCGTCAGTTAATGTCCAGGA2830.26994028882657056No Hit
CCCGTTCGCTCCCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCCAGAA2820.2689864362158759No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTGCCGTCCAGTA2680.2556324996661516No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGTA2600.24800167878059484No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATCGTCCAGTA2590.24704782616990023No Hit
CCTGTTTGATACCCGCACCTTCGAGCCTCAGCGTCAGTTGCGCTCCGGAC2470.23560159484156507No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGTTGCCCAGTA2430.2317861843987867No Hit
CCTGTTTGCTCCCCATGCTTTCGTGCCTCAGCGTCAGTTAGAGCCCAGCA2390.22797077395600832No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA2330.22224765829184073No Hit
CCTGTTTGCTACCCGCACTTTCGAGCCTCAACGTCAGTTACGGTCCAGCA2220.21175527957420018No Hit
CCTGTTTGCTACCCACACTTTCGAGCATGAACGTCAGTTACGGCTTAGTG2140.20412445868864343No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAACGTCAGTTACCGTCCAGTA2140.20412445868864343No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTCCAGTA2120.20221675346725423No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGCA2010.19172437474961368No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGTT1900.18123199603197315No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAACGTCAGTGGCGGTTTTGTT1880.17932429081058396No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGAGCCCAGTA1870.17837043819988935No Hit
CCTGTTTGATACCCGCACTTTCGCGCATCAACGTCAGTTACGGTCCAGCA1790.1707396173143326No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA1740.1659703542608596No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAACGTCAGTGGCGGTTTAGCA1620.15452412293252446No Hit
CCTGTTTGATACCCGCACTTTCGAGCATCAACGTCAGTTACAGTCCAGCA1620.15452412293252446No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAG1470.14021633377210554No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAACGTCAGTGGCGGTTTAGTA1460.13926248116141093No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTTCAGTCCAGTA1450.13830862855071635No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA1420.13544707071863255No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGCTGTCCAGCA1420.13544707071863255No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGAG1420.13544707071863255No Hit
CCTGTTTGCTCCCCACACTTTCGTGTCTCAGCGTCAGGTATCGTCCAGTA1410.13449321810793796No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTA1410.13449321810793796No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTGCTGTCCAGCA1250.11923157633682442No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTACAGCCCAGCA1230.11732387111543524No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGTA1230.11732387111543524No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACGGCCCAGCA1220.11637001850474063No Hit
CCTGTTTGCTACCCACACTTTCGAGCATCAACGTCAGTTACAGCTTAGCA1190.11350846067265687No Hit
CCTGTTCGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTGGCGGCCCAGAA1190.11350846067265687No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAACGTCAGTTACGGTCCAGCA1180.11255460806196226No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGTG1180.11255460806196226No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAACGTCAGTGGCGGTTTTGCT1140.10873919761918388No Hit
CCTGTTCGATACCCGCGCTTTCGAGCCTCAGCGTCAGTTGCGCTACCGTC1140.10873919761918388No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTATAGCCCAGCA1090.10396993456571091No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTTCAGTCCAGTA1060.10110837673362712No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)