FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001104983

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001104983
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74961
Sequences flagged as poor quality0
Sequence length251
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA52927.059671028935047No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA39305.2427262176331695No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTCCAGTA31884.252878163311589No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA31744.234201784928163No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA26703.5618521631248248No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGTA22873.0509198116353837No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA18322.443937514174037No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA15742.099758541108043No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGCA10101.3473672976614508No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTGCCGTCCAGTA9811.308680513867211No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA8941.1926201624844919No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCACTGTCAGTTGCAGCTTAGCA7400.9871800002668053No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA6970.9298168380891397No Hit
CCCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCCAGAA6580.8777897840210244No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTG6450.8604474326649857No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA6360.8484411894184977No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAACAGTCCAGCA5940.7924120542682194No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGTA5910.7884099731860568No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA5280.7043662704606395No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATCGTCCAGTA5020.6696815677485626No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCA4780.6376649190912608No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTCATGTCCCAGAG4750.6336628380090981No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACACTCCAGTG4690.6256586758447726No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTGAACGTCAGTTACTGTCCAGCA4210.561625378530169No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA4060.5416149731193554No Hit
CCCTTTCGCTCCCCTGGCCTTCGTGCCTCAGCGTCAGTTAATGTCCAGGA3980.5309427569002547No Hit
CCTGTTTGATACCCACACTTTCGCGCCTCAATGTCAGTTGCAGCTTAGCA3830.5109323514894412No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTA3520.4695775136404264No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGTGTCAGTTATGGTTTAGTA3410.4549032163391631No Hit
CCTGTTTGATCCCCATGCTTTCGTGCCTCAGCGTCAGTTGACAGCCAGAT3340.44556502714745No Hit
CCTGTTTGATCCCCATGCTTTCGTGCCTCAGCGTCAGTATATGGCCAGAT3020.40287616227104756No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAACGTCAGTTACCGTCCAGTA2960.3948720001067222No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGTA2750.3668574325315831No Hit
CCTGTTTGCTACCCACACTTTCGCGCCTCAGCGTCAGTTGGTGCCCAGTA2640.35218313523031974No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTAATCGTCCAGTA2640.35218313523031974No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGTA2590.3455130000933819No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTTAGTGTCAGTTTCAGTCCAGTA2560.34151091901121916No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA2300.3068262162991422No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAGGCCCAGTA2180.29081789197049135No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTAACAGTCTAGTG1880.25079708114886407No Hit
CCTATTTGCTCCCCACGCTTTCGGGACTGAGCGTCAGTTATGCGCCAGAT1880.25079708114886407No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTACAGTCCAGTT1800.24012486492976348No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTA1740.232120702765438No Hit
CCTGTTTGCTACCCACGCTTTCGCACCTCAGCGTCAGTTACCGTCCAGGT1670.222782513573725No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAACGTCAGTTACAGTCCAGTA1670.222782513573725No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG1610.21477835140939955No Hit
CCTGTTTGATCCCCATGCTTTCGAGCCTCAGCGTCAATATACAGCCAGAT1440.19209989194381077No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA1330.17742559464254745No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGCCCAGTA1320.17609156761515987No Hit
CCTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCTGTCGCACCCTAGCA1310.1747575405877723No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCATTGTCAGTCCAGTA1300.17342351356038474No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAGGCCCAGCA1280.17075545950560958No Hit
CCTGTTTGATACCCACACTTTCGCGCCTCACTGTCAGTTGCAGCTTAGCA1270.169421432478222No Hit
CCTGTTTGCTACCCATGCTTTCGCGCCTCAGCGTCAGTTGGTGCCCAGTA1190.15874921625912142No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTACAGTCCAGCA1160.15474713517695868No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCA1150.1534131081495711No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAACGTCAGTTGCCGTCCAGTA1110.1480770000400208No Hit
CCTGTTTGATCCCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA1040.13873881084830778No Hit
CCTGTTTGCTCCCCACGCTCTCGAGCCTCAGCGTCAGTTATCGTCCAGTA1040.13873881084830778No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA1040.13873881084830778No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCCTAGCA960.1280665946292072No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTTCAGTCCAGTA910.1213964594922693No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGTA880.1173943784101066No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTATCGTCCAGTA860.11472632435533144No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTACAGTCCAGTG860.11472632435533144No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTTCAGTCCAGTA830.11072424327316871No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA820.10939021624578114No Hit
CCTGTTTGATACCCACACTCTCGAGCCTCAATGTCAGTTGCAGCTTAGCA800.10672216219100598No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTCAGTCCAGAA790.10538813516361842No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTT780.10405410813623085No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCGGTCTCGGCCCAGAG780.10405410813623085No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA760.10138605408145569No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAATGTCAGTTACAGTCCAGTA760.10138605408145569No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)