FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001105459

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001105459
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20334
Sequences flagged as poor quality0
Sequence length251
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGTTCGCTACCCTAGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAA438121.545195239500345No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG231411.379954755581783No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG17578.640700304908036No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGAA6323.1080948165633915No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGT5882.891708468574801No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGAA4362.1441919937051246No Hit
CCCGTTCGCTACCCTCGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAA3551.74584439854431No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAA2161.0622602537621717No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGTG1830.8999704927707288No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAG1680.8262024195928003No Hit
TCCGTTCGCTACCCTAGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAA1560.7671879610504574No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGTG1010.4967050260647192No Hit
TCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG980.48195141142913345No Hit
CCCGTTCGCTACCCTAGCTTTCGCGCCTCAGCGTCAGTTACAGTCCAGAA830.4081833382512049No Hit
TCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG760.37375823743483816No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGTCCAGAG710.349168879708862No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGA660.3245795219828858No Hit
CCCGTTCGCTACCCTAGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGCA660.3245795219828858No Hit
CCTGTTCGCTACCCTAGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAA600.29507229271171437No Hit
CCCGTTCGCTACCCTAGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAC580.2852365496213239No Hit
CCCGTTCGCTACCCTAGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG560.2754008065309334No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG560.2754008065309334No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGTA550.27048293498573817No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA520.25572932035015244No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGTG490.24097570571456672No Hit
CCCGTTCGCTACCCTCGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGTA440.21638634798859055No Hit
CCCGATCGCTACCCTAGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAA420.20655060489820007No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGTT410.20163273335300483No Hit
CTCGTTCGCTACCCTAGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAA410.20163273335300483No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGCCCAGAG400.1967148618078096No Hit
ACCGTTCGCTACCCTAGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAA390.19179699026261435No Hit
TCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGT390.19179699026261435No Hit
CCCGTTCGCTACCCTAGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGTA390.19179699026261435No Hit
CCCGTTCGCTACCCTAGCTTTCGAGCCTCAGCGTCAGTTACAGTCCCGAA380.18687911871741908No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGTA350.17212550408183339No Hit
CCTGATTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG340.16720763253663815No Hit
CCTGTTCGCTACCCTCGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAA320.15737188944624766No Hit
CCCGTTAGCTACCCTAGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAA320.15737188944624766No Hit
CCTGATTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG310.15245401790105242No Hit
CCCGTTCGCTACCCTCGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGCA300.14753614635585718No Hit
CACGTTCGCTACCCTAGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAA300.14753614635585718No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG290.14261827481066194No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAT290.14261827481066194No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGTCCAGAG290.14261827481066194No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA290.14261827481066194No Hit
CCCGTTCGCTACCCAAGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAA280.1377004032654667No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTCTTCGTCCAGGG280.1377004032654667No Hit
CCCGTTCGCTACCCTAGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGAA280.1377004032654667No Hit
CCCGTTCGCTACCCTCGCTTTCGCGCCTCAGCGTCAGTTACAGTCCAGAA260.12786466017507622No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTA240.11802891708468574No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG230.1131110455394905No Hit
CCCGTTCGCTACCCTAGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAT230.1131110455394905No Hit
CCCGTTCGCTACCCTAGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAG230.1131110455394905No Hit
CATGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG220.10819317399429527No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTACAGTCCAGAA220.10819317399429527No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTGAGCGTCAGTCTTCGTCCAGGG220.10819317399429527No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGTA220.10819317399429527No Hit
CCCGTTCGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAA220.10819317399429527No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)