FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001105533

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001105533
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37617
Sequences flagged as poor quality0
Sequence length251
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGTGTCAGTTATAGTTTAGTA384510.221442432942553No Hit
CCGGTTCGCGCCCCTAGCTTTCGTCCCTCACCGTCAGAATCGTTCCAGTC23346.204641518462398No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTAGTGCCCAGTA12473.314990562777468No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA8672.30480899593269No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACACTCCAGTG7782.068213839487466No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA7291.937953584815376No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG6981.8555440359411968No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA6281.6694579578382114No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA6071.6136321344073157No Hit
CCGGTTCGCTCCCCTAGCTTTCGTCCCTCACCGTCAGAATCGTTCCAGTC5201.3823537230507483No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCA4501.196267644947763No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGCA4171.1085413509849271No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGCGTCAGTTATAGTTTAGTA3520.9357471356035836No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA3430.9118217827046282No Hit
CCTGTTTGATCCCCACGCTTTCGCGCTTCAGTGTCAGTTATAGTTTAGTA3390.9011882925273149No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG3360.8932131748943297No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAGAGCCCAGCA3340.887896429805673No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACTGTCCAGCA3310.8799213121726879No Hit
TCTGTTTGATCCCCACGCTTTCGTGCTTCAGTGTCAGTTATAGTTTAGTA3210.8533375867294043No Hit
CCTGTTTGCTACCCACGCTTTCGCGCCTCAGCGTCAATACCGGTCCAGAC2970.7895366456655235No Hit
CCTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCTGTCGCACCCTAGCA2880.7656112927665684No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA2730.7257357046016428No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA2410.6406677831831352No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA2400.638009410638807No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCATTCACCGTCCAGTA2070.550283116675971No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGAG2000.5316745088656725No Hit
CCTGTTTGCTACCCATGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGTA1690.44926495999149324No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCA1550.4120477443708961No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA1440.3828056463832842No Hit
CCTGTTCGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTGGCGGCCCAGAA1430.3801472738389558No Hit
CCGGTTCGCGCCCCTGGCTTTCGTTACTCACCGTCAAGATCGTTCCAGTT1370.36419703857298563No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTTGTCCAGGG1300.3455884307626871No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGTGTCAGTTATGGTTTAGTA1240.3296381954967169No Hit
CCCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCCAGAA1220.3243214504080602No Hit
CCTATTTGCTCCCCACGCTTTCGGGACTGAGCGTCAGTTATGCGCCAGAT1220.3243214504080602No Hit
CCTGTTTGCTACCCACGCTTTCGCACCTCAGCGTCAGTTACCGTCCAGGT1210.3216630778637318No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCACTGTCCAGTA1110.2950793524204482No Hit
CCCGTTCGCTGCCCTGGCTTTCGCACATGAGCGTCAGTTGTGTGCCAGGA1090.2897626073317915No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACTGTCCAGAA1040.2764707446101497No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTGCAGTCCAGTG980.2605205093441795No Hit
TCGGTTCGCGCCCCTAGCTTTCGTCCCTCACCGTCAGAATCGTTCCAGTC940.24988701916686604No Hit
CCTGTTCGCTACCCACGCTCTCGCGCATCAGCGTCAGAACGAGCCTGGGA930.24722864662253766No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGTA900.2392535289895526No Hit
CCTGTTCGCTACCCACGCTCTCGCGCATCAGCGTCAGATCGAGCCTGGGA880.2339367839008959No Hit
CCTGTTTGATCCCCACGCTTTCGCGCTTCAGCGTCAGTTATAGTTTAGTA870.23127841135656751No Hit
CCTATTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGACCAGGC830.22064492117925405No Hit
CCGGTTCGCGCCCCTCGCTTTCGTCCCTCACCGTCAGAATCGTTCCAGTC820.2179865486349257No Hit
CCTGTTTGCTACCCACGCTTTCGCGCCTCAGCGTCAGTTAAAGCCCAGCA810.21532817609059732No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCGGTCTCGGCCCAGAG800.212669803546269No Hit
CCGGTTCGCGCCCCTAGCTTTCGCCCCTCACCGTCAGAATCGTTCCAGTC780.20735305845761223No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGTGTCAGTTATAGTTTAGTA750.19937794082462718No Hit
TCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTAGTGCCCAGTA680.18076933301432863No Hit
CCTGTTTGCTCCCCATGCTTTCGTGCCTCAGCGTCAGTTAGAGCCCAGCA640.1701358428370152No Hit
CCTGTTCGCTACCCACGCTCTCGTGCATCAGCGTCAGTTACAGTCTGGTA640.1701358428370152No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTATCGTCCAGTA610.1621607252040301No Hit
CCGGTTCGCTCCCCTAGCTTTCGCCCCTCACCGTCAGAATCGTTCCAGTC610.1621607252040301No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGAGTCAGTTATAGTTTAGTA580.15418560757104502No Hit
CCGGTTCGCTCCCCTCGCTTTCGTCCCTCACCGTCAGAATCGTTCCAGTC580.15418560757104502No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCACTGTCCAGCA570.15152723502671664No Hit
CCTGTTTGCTCCCCATGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA560.1488688624823883No Hit
TCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA540.14355211739373155No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAACGTCAGTTACAGTCCAGTA530.1408937448494032No Hit
TCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG530.1408937448494032No Hit
CCTGTTTGCTACCCATGCTTTCGCGCCTCAGCGTCAGTTAGTGCCCAGTA520.13823537230507485No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGCA520.13823537230507485No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTGCTGTCCAGCA520.13823537230507485No Hit
CCTGTTCGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTTGCGGCCCAGCA500.13291862721641812No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGAG500.13291862721641812No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCATTTACAGTCCAGTA500.13291862721641812No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCTTCAGTGTCAGTTATAGTTTAGTA490.13026025467208974No Hit
CCTGTTTGATCCCCACGCTTTCGTGCCTCAGTGTCAGTTATAGTTTAGTA470.12494350958343302No Hit
TCGGTTCGCTCCCCTAGCTTTCGTCCCTCACCGTCAGAATCGTTCCAGTC450.1196267644947763No Hit
CCTGTTTGATACCCACACTTTCGCGCATCAGCGTCAGTTACACTCCAGTG450.1196267644947763No Hit
TCTGTTTGATCCCCACGCTTTCGCGCTTCAGTGTCAGTTATAGTTTAGTA440.11696839195044795No Hit
CCTGTTTGATACCCACACTTTCGCGCATCAGTGTCAGTTGCAGTCCAGTG440.11696839195044795No Hit
TCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACACTCCAGTG430.11431001940611957No Hit
CCTGTTTGCTACCCACACTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGTA430.11431001940611957No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTAACAGTCCAGTA430.11431001940611957No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGTA420.11165164686179122No Hit
TCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA410.10899327431746285No Hit
CCTGTTTGATCCCCACGCTTTCGAGCTTCAGTGTCAGTTATAGTTTAGTA410.10899327431746285No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTAGAGCCCAGTA400.1063349017731345No Hit
TCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA390.10367652922880612No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCCTAGCA390.10367652922880612No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGCCGTCCAGTT390.10367652922880612No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGAA390.10367652922880612No Hit
TCTGTTTGATCCCCACGCTTTCGTGCTTCAGCGTCAGTTATAGTTTAGTA380.10101815668447776No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)