FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001105743

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001105743
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68415
Sequences flagged as poor quality0
Sequence length251
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTTCGCTCCCCTGGCCTTCGTGCCTCAGCGTCAGTTAATGTCCAGGA23443.4261492362785937No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA22013.217130746181393No Hit
CCCGTTCGCTGCCCTGGCTTTCGCACATGAGCGTCAGTAGTGTGCCAGGA19642.870715486369948No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGCA19192.804940437038661No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA19052.7844770883578165No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA16362.3912884601330116No Hit
CCTGTTCGATACCCACGCCTTCGAGCTTCAGCGTCAGTGCGGCGCTGGCA14512.120879923993276No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA10771.5742161806621353No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA9961.455821091865819No Hit
CCCGTTCGCTACCCACGCTTTCGCTCCTCAGCGTCAGAGACAGCCCAGAG9901.447051085288314No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA9851.4397427464737265No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAGAGCCCAGCA7281.0640941314039318No Hit
CCTGTTCGATACCCGCACCTTCGAGCCTCAACGTCAGTCCAGGCCTGGCA6961.0173207629905723No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACGGCCCAGTA6891.0070890886501498No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGTA6690.9778557333918001No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGCTGTCCAGCA6620.9676240590513776No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA6090.8901556676167507No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGCA5960.8711539866988234No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGTA5290.7732222465833516No Hit
CCTGTTCGATACCCGCGCTTTCGAGCCTCAGCGTCAGTTGCGCTCCGGTA4890.7147555360666521No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCCGATATAGATTTGTA4860.7103705327778996No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAACGTCAGTTGCAGCTTAGCA4470.6533654900241175No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACTGTCCAGCA4350.6358254768691076No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCA4310.6299788058174377No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA4110.6007454505590879No Hit
CCTGTTTGCTACCCACGCTTTCGCACCTCAGCGTCAGTTACCGTCCAGGT3970.5802821018782431No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACGGCCCAGCA3970.5802821018782431No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA3890.5685887597749031No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCA3780.5525104143828108No Hit
CCGGTTCGCGCCCCTAGCTTTCGTCCCTCACCGTCAGAATCGTTCCAGTC3610.5276620624132135No Hit
CCCTTTCGCTCCCCTGGCCTTCGCGCCTCAGCGTCAGTTAATGTCCAGGA3360.4911203683402763No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA3230.4721186874223489No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTAACAGTCCAGTA3190.466272016370679No Hit
CCTGTTTGCTACCCATGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGTA3120.45604034203025656No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCCGTGCCGGCGCGGCA2780.4063436380910619No Hit
CCTATTTGCTCCCCACGCTTTCGTGACTGAGCGTCAGTATCGTCCTGGTG2570.37564861506979463No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTATTATCCCAGGA2540.37126361178104217No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTCCGGTCCAGCA2490.36395527296645475No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG2430.3551852663889498No Hit
CCTGTTCGATACCCGCACCTTCGAGCATCAGCGTCAGTAATGCTGCGGCA2350.3434919242856099No Hit
CCTGTTTGATACCCGCACCTTCGAGCCTCAACGTCAGTGGCGGACTGGCA2260.3303369144193525No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGTCCAGAA2150.3142585690272601No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACACTCCAGTG2130.3113352335014251No Hit
CCTGTTCGATACCCGCACCTTCGAGCCTCAACGTCAGTCACGGACTGGCA2080.3040268946868377No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAACGTCAGTTGCAGCTTAGCA1820.26602353285098296No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAATACCGGTCCAGGT1820.26602353285098296No Hit
CCCGTTCGCTACCCTAGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAA1780.26017686179931304No Hit
CCCTTTCGCTCCCCTGGCCTTCGTGCCTCAGCGTCAGTTAATGTCCAGTA1620.23679017759263318No Hit
CCCGTTTGCGACCCAGGGCTTCGTCCCTCACCGTCGGATCCGTTCTAGCA1580.23094350654096324No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTTTTGCCCCAGGG1570.22948183877804576No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTAAAAGCCCAGCA1530.2236351677263758No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAACGTCAGTTACAGTCCAGAA1510.2207118322005408No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGTA1470.2148651611488709No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAACGTCAGATATAGTTTGGTA1470.2148651611488709No Hit
CCCGTTCGCTGCCCTGGCTTTCGCACATGAGCGTCAGTAGTGTTCCAGGA1440.21048015786011837No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGCA1410.20609515457136593No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG1390.20317181904553094No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTGTCAGTCCAGAA1370.20024848351969599No Hit
CCCGTTCGCTGCCCTGGCTTTCGCACATGAGCGTCAGTTGTGTGCCAGGA1330.19440181246802601No Hit
CCTGTTCGATACCCACGCTTTCGTGCCTCAGTGTCAGTCATGGCTTGGTA1330.19440181246802601No Hit
CCTGTTTGCTACCCATGCTTTCGCGCCTCAGCGTCAGTTGGTGCCCAGTA1230.1797851348388511No Hit
CCTGTTCGCTACCCACGCCTTCGAGCTTCAGCGTCAGTGCGGCGCTGGCA1040.15201344734341884No Hit
CCTGTTCGCTACCCACGCTTTCGTGCATCAGCGTCAGATATAGATTAGCA1030.15055177958050137No Hit
CCTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCTGTTTCACCCTAGCA1000.14616677629174887No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGCCGTCCAGTT980.14324344076591392No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA960.14032010524007893No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTAAGGCCCAGCA960.14032010524007893No Hit
CCTGTTCGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTTCCAGTCCAGA950.13885843747716142No Hit
CCCGTTCGCTCCCCTCGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA920.13447343418840899No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATTGTCCAGCA900.131550098662574No Hit
CCTGTTCGATACCCACGCCTTCGCGCTTCAGCGTCAGTGCGGCGCTGGCA860.12570342761090403No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAACGTCAGTTACTGTCCAGTA850.12424175984798655No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTAGTGCCCAGTA850.12424175984798655No Hit
CCCTTTCGCTCCCCTCGCCTTCGTGCCTCAGCGTCAGTTAATGTCCAGGA850.12424175984798655No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCCGATATAGATTTGTA840.12278009208506907No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA830.12131842432215158No Hit
CCTGTTTGATACCCGCACCTTCGAGCCTCAACGTCAGTGGCGGGCCTGCA830.12131842432215158No Hit
CCTGTTCGATACCCACGCCTTCGAGCTTCAGCGTCAGTGCGGCTCTGGCA820.1198567565592341No Hit
CCTGTTTGCTACCCACACTTTCGCGCCTCAGCGTCAGTTGGTGCCCAGTA790.11547175327048162No Hit
CCTGTTCGCTACCCGCACCTTCGAGCCTCAACGTCAGTCCAGGCCTGGCA770.11254841774464663No Hit
CCGGTTCGCTCCCCTAGCTTTCGTCCCTCACCGTCAGAATCGTTCCAGTC750.10962508221881166No Hit
CCTGTTTGCTACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGTA750.10962508221881166No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCGGTCTCGGCCCAGAG750.10962508221881166No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGCGGCCCAGTA740.10816341445589418No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAACGTCAGTTACTGTCCAGCA720.10524007893005918No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATCGTCCAGCA720.10524007893005918No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGTCCAGAA720.10524007893005918No Hit
CCCGTTCGCTACCCTGGCTTTCGCACATGAGCGTCAGTAGTGTGCCAGGA710.1037784111671417No Hit
CCCTTTCGCTCCCCTGGCCTTCGCGCCTCAGCGTCAGTTAATGTCCAGTA690.10085507564130673No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)