FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001106081

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001106081
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74823
Sequences flagged as poor quality0
Sequence length251
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCCAGTG68439.145583577242292No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA36184.8354115713082875No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA21782.910869652379616No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAGCAGTCCAGTA19642.624861339427716No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA19582.6168424147655136No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTCCAGTA18672.4952220573887707No Hit
CNTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCCAGTG16382.189166432781364No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA12771.7066944656054956No Hit
CNTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA8881.186800850006014No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA7491.0010290953316494No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAACAGTCCAGCA5750.7684802801277683No Hit
CNTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA5230.6989829330553439No Hit
CCTGTTCGATACCCGCACTTTCGCGCTTCAGCGTCAGTTGCGCTCCAGTG5160.6896275209494407No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA4640.6201301738770164No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGCA4630.618793686433316No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA4560.6094382743274127No Hit
CNTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA4480.598746374777809No Hit
CNTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTCCAGTA4410.5893909626719057No Hit
CNTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAGCAGTCCAGTA4370.5840450128971039No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA3690.49316386672547213No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTG3620.4838084546195689No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA3490.4664341178514628No Hit
CNTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA3450.461088168076661No Hit
CCTATTTGCTCCCCACGCTTTCGGGACTGAGCGTCAGTTATGCGCCAGAT3030.4049556954412413No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGATGCGCTCCAGTG3000.4009462331101399No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA2950.39426379589163757No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGTA2930.39159082100423664No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTCATGTCCCAGAG2920.39025433356053624No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGAG2860.3822354088983334No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGTA2610.34882322280582173No Hit
CCTGTTTGCTACCCACACTTTCGCGCCTCAGCGTCAGTTGGTGCCCAGTA2540.33946781069991844No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCA2450.3274394237066143No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTA2410.3220934739318124No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTATCGTCCAGCA2290.3060556246074068No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGTGTCAGTTATGGTTTAGTA2280.30471913716370636No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTGCCGTCCAGTA2270.3033826497200059No Hit
CNTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA2200.2940272376141026No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTCATCGTCCAGTA2010.26863397618379375No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAACGTCAGTTACCGTCCAGTA1810.24190422730978445No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCA1810.24190422730978445No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA1680.22452989054167838No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATCGTCCAGTA1680.22452989054167838No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCTAGTG1630.217847453323176No Hit
CCTGTTCGATACCCGCACTTTCGAGCTCCAGCGTCAGTTGCGCTCCAGTG1560.20849204121727274No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGATGGTGCCCAGTA1560.20849204121727274No Hit
CNTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAACAGTCCAGCA1540.20581906632987182No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACACTCCAGTG1500.20047311655506994No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTA1450.19379067933656763No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGTTGCCCAGTA1430.1911177044491667No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA1430.1911177044491667No Hit
CCTGTTCGACACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCCAGTG1360.18176229234326344No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA1290.17240688023736017No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTAATCGTCCAGTA1220.1630514681314569No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG1210.16171498068775644No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATCGTCCAGTT1110.14835010625075176No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTGCAGTCCAGTA1110.14835010625075176No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAGGCCCAGTA1100.1470136188070513No Hit
CCTGTTTGATACCCACACTTTCGCGCCTCAATGTCAGTTGCAGCTTAGCA1090.14567713136335084No Hit
CCTATTTGCTCCCCACGCTTTCGCGACTGAGCGTCAGTTATGCGCCAGAT1080.14434064391965037No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGTA1040.13899469414484852No Hit
CNTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA1040.13899469414484852No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCA1030.13765820670114803No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG1030.13765820670114803No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCGGTCTCGGCCCAGAG1010.1349852318137471No Hit
CNTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGCA1000.13364874437004665No Hit
CNTATTTGCTCCCCACGCTTTCGGGACTGAGCGTCAGTTATGCGCCAGAT990.13231225692634618No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTACCGTCCAGTA990.13231225692634618No Hit
CCTTTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCCAGTG960.1283027945952448No Hit
CCTGTTCGATACCCGCACTTTCGAGCATCAGCGTCAGTTGCGCTCCAGTG960.1283027945952448No Hit
CNTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTG950.1269663071515443No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGATATCGTCCAGTA880.11761089504564105No Hit
CCTGTTCGCTACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCCAGTG860.11493792015824013No Hit
CNTGTTCGATACCCGCACTTTCGCGCTTCAGCGTCAGTTGCGCTCCAGTG850.11360143271453965No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGCA850.11360143271453965No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCACTGTCAGTTGCAGCTTAGCA840.11226494527083919No Hit
CNTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA830.1109284578271387No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTGTCGTCCAGTA800.10691899549603731No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCATTGTCAGTCCAGTA790.10558250805233683No Hit
CNTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA770.10290953316493591No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCACTCCAGTG770.10290953316493591No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACTGTCCAGAA760.10157304572123545No Hit
CCTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCTGTCGCACCCTAGCA750.10023655827753497No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)