FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001106601

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001106601
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66902
Sequences flagged as poor quality0
Sequence length251
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA42106.292786463782847No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA41376.183671639113928No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAATCCAGTA34135.10149173417835No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA24873.7173776568712444No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA20893.122477653881797No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA15032.2465696092792444No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTG12471.8639203611252277No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA10881.6262593046545692No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG9981.491734178350423No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA8941.336282921287854No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTTATGTCCCAGGA7651.143463573585244No Hit
CCCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCCAGAA7641.1419688499596423No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCACTGTCAGTTGCAGCTTAGCA7331.0956324175659922No Hit
CCTGTTTGCTACCCATGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGTA7121.064243221428358No Hit
CCTGTTTGCTACCCACACTTTCGAGCATCAGCGTCAGTTACAATCCAGTA5490.8206032704552928No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGTGTCAGTGACCAGTCTAGT5380.8041613105736749No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA5330.7966876924456668No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA5260.7862246270664555No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA5100.7623090490568294No Hit
CCTGTTTGCTACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA4410.6591731188903172No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGTA4340.6487100535111058No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGTGTCAGTTATGGTTTAGTA3980.5949000029894472No Hit
CCTGTTTGCTACCCACACTTTCGCGCCTCAGCGTCAGTTGGTGCCCAGTA3650.5455741233445937No Hit
CCTGTTTGATACCCACACTTTCGCGCCTCAATGTCAGTTGCAGCTTAGCA3470.5186690980837643No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTA3320.4962482436997399No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAGTCGTCCAGTA3280.4902693491973334No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAACAGTCCAGCA3250.48578517832052853No Hit
CCTGTTTGATACCCACACTTTCGCGCATCAGCGTCAGTTACAATCCAGTA3240.48429045469492693No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTGAACGTCAGTTACTGTCCAGCA3210.479806283818122No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAATGTCAGTTACAGGTTAGTA3170.47382738931571555No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTATCGTCCAGCA2940.4394487459268781No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTCCAGTA2800.4185226151684553No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTGCCGTCCAGTA2590.3871334190308212No Hit
CCTGTTTGATACCCACACTTTCGCGCATCAGCGTCAGTTACAGTCCAGCA2580.38563869540521956No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA2570.38414397177961795No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAGAGCCCAGCA2490.3721861827748049No Hit
CCCTTTCGCTCCCCTGGCCTTCGTGCCTCAGCGTCAGTTAATGTCCAGGA2240.33481809213476427No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCA2070.3094077904995366No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCACTGTCCAGTA2020.30193417237152853No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAACGTCAGTTACCGTCCAGTA1860.2780185943619025No Hit
CCTGTTTGCTACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG1850.27652387073630086No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCACTGTCAGTTGCAGCTTAGCA1540.23018743834265043No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGTA1520.22719799109144717No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCA1480.22121909658904068No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTAACAGTCTAGTG1440.2152402020866342No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA1380.20627186033302441No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGTA1370.2047771367074228No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTACCGTCCAGTA1330.19879824220501627No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATCGTCCAGTA1310.19580879495381304No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCTAGTG1300.1943140713282114No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACACTCCAGTG1260.18833517682580492No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTGCTGTCCAGCA1230.18385100594900003No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTCATGGCTTGGCA1220.1823562823233984No Hit
CCTGTTTGATACCCACACTTTCGCGCCTCACTGTCAGTTGCAGCTTAGCA1190.1778721114465935No Hit
CCTGTTTGCTACCCACACTTTCGCGCATCAGCGTCAGTTACAATCCAGTA1180.1763773878209919No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTACAGTCCAGTG1180.1763773878209919No Hit
CCTGTTTGATACCCACACTTTCGCGCATCAGTGTCAGTTGCAGTCCAGTG1140.1703984933185854No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGTA1120.16740904606738213No Hit
CCTGTTTGCTACCCACACTTTCGCGCATCAGCGTCAGTTACAGTCCAGCA1080.16143015156497564No Hit
CCTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCTGTTTCACCCTAGCA1070.15993542793937401No Hit
CCTGTTTGCTACCCACACTTTCGCGCCTCAATGTCAGTTGCAGCTTAGCA1000.1494723625601626No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA900.13452512630414637No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA890.13303040267854474No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCTTCAGTGTCAGTTATGGTTTAGTA860.12854623180173988No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCATTGTCAGTCCAGTA840.1255567845505366No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGCCGTCCAGTT820.12256733729933335No Hit
CCTGTTTGCTACCCACACTCTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA820.12256733729933335No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTCATGGCTTTGCA810.12107261367373173No Hit
CCTATTTGCTCCCCACGCTTTCGGGACTGAGCGTCAGTTATGCGCCAGAT810.12107261367373173No Hit
CCTGTTTGATACCCACACTCTCGAGCCTCAATGTCAGTTGCAGCTTAGCA800.1195778900481301No Hit
CCTGTTTGCTACCCACGCTTTCGCACCTCAGCGTCAGTTACCGTCCAGGT750.11210427192012197No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAACGTCAGTTACAGTCCAGTA740.11060954829452034No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTGGTCGTCCAGTA730.10911482466891873No Hit
CCTGTTTGCTACCCATGCTTTCGCGCCTCAGCGTCAGTTGGTGCCCAGTA720.1076201010433171No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGTA710.10612537741771548No Hit
CCTGGTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA690.10313593016651221No Hit
CCTGTTTGATACCCACACTTTCGAGCATCACTGTCAGTTACAGGTTAGTA690.10313593016651221No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)