FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001109450

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001109450
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2315141
Sequences flagged as poor quality0
Sequence length35-151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT917793.9642941833780316TruSeq Adapter, Index 14 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATATCGTAT41510.17929793476941577TruSeq Adapter, Index 14 (97% over 43bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)