FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001109584

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001109584
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1726043
Sequences flagged as poor quality0
Sequence length35-151
%GC40

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT184191.067122893230354TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATATCGTAT44470.2576413217978926TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)