FastQCFastQC Report
Wed 8 May 2024
EGAF00001128947

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001128947
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2396593
Total Bases359.4 Mbp
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATCACTGCCAAGTTAGACAGCCCCAGGGTGACAGTGGCCCAGCTGGAC113760.47467383907071414No Hit
CGATGACAGCGCCTTCATGGAGCCGAGGTACCACGTCCGTCGAGAAGATC57020.2379210821361825No Hit
TGGTGATGGGTGTCGATGTTGGGGTCTGTGTGCCGGTGGGTGTTGGGGTT56550.23595996483341142No Hit
GGGAGCGTCACGTCTACCCGCCAGTTCCAGTCATAGCTGACTTGCAGTCC45070.18805863156572683No Hit
GCCGTAAAACCTCCAGTGACCCGTTTGCCTTCCCTCCTTCCCCCAGGTGA41880.17474806944691904No Hit
GCATGCACCCTCCTAACTGAGGTCTGGTGGGAGCCATGGAAGGACCAGGG40050.1671122297361296No Hit
GGTCTCTTGCAGTCTGGAAGAGGACGGGCTCGTGGACAGAGGGCGTGGCC38560.16089507062734473No Hit
GGTAGAGCTGCAAGGACACCGCCTGCACAGGGTGCCCGGGGCTGGGCATT37350.15584623672021072No Hit
GGTGAAGCAGTGTCCTTGTCCCAGGGGCTGCTGCTGTTGCTGCTGCTGAG36010.15025496611231026No Hit
GACTCTTATCCATCCGTCCTCTCCCAGCTCACCAATCACTCGGCCTAGGC35160.14670826460729877No Hit
GGAGGATTCTGTGGCGTATGAAGACCACGGGATAACCGTATTGCCCAAGA34350.1433284667025231No Hit
AAGCATTCAAGTAGCAGGTATTTCCCATATTCTGGAGCCCAGCCCCCACC33910.14149252709992893No Hit
CCATGGCTATCCCCATCTGGGTCTGGCAGAAGTTCTCCTCCAGTTGTGCA33640.14036592779833706No Hit
GCACCAAGCCAAGAAAGTCCAGCAGGAGCACGAGCATCCCGATGATCACG33390.1393227802968631No Hit
GACTCTTATCCATCCGTCCTCTCCCAGCTCACCAATCACTTGGCCTAGGC31420.13110277798524822No Hit
AAGGTGCTGACTTCCTTGGGAGATGCCATAAAGCACCTGGATGATCTCAA30700.1280985131810032No Hit
CGGAGCATGTAGGTGTAGAAGGAAGCACCGAAGAGCAGCAGCACTATGAT30690.12805678728094425No Hit
GCCCCACCAAGCCAGATAGGAAGGCCACTGTTGGGAGGCAGGAGGGAAAG30580.1275978023802957No Hit
AGGGTCACAGATCTTTAACAAGAATGGGGGCACTCAGGGTCTGAGGCCAC30570.12755607648023673No Hit
GGCGGCAGAATTCATCCTGTGGATGAGATGCAGGATGAGGGTGTATGAGC30460.1270970915795882No Hit
CTGTGATGATTTGGCTCCTGTGGCAAGACAGCTTGCTCCCAGGGAGAAGC29970.1250525224766992No Hit
GAGAACTCCTTTGTCCATTTGCACGTATGGATCTTTCCTCACTGCTTGAT29960.12501079657664024No Hit
GAGGTTTGTTGCCTCCTTGTGGGGGTGGTCCTTGTGGCTTTCCTGGAGGA29920.12484389297640441No Hit
GATATGCTTTATGGTCTGCTCAGTCACCTCGTTCTTAATCCCTTTCTCAA29690.12388419727504837No Hit
ACAAGGAGGCAACAAACCTCAAGGTCCCCCACCTCCAGGAAAGCCACAAG29540.123258308774164No Hit
GGGCTGGACATCTTGAAGTGCCGCACCACAAAGTCCTTGCCGCCCATCAT29230.12196480587233628No Hit
GCTGGTTGCCTCCTTGTGGGGGTGGTCCTTGTGGCTTTCCTGGAGGAGAT29130.1215475468717467No Hit
CAGAGCCTGGAAATGGTTGAGACTACCCTGCCACGATTTTGCTCCCGCTC27790.11595627626384622No Hit
GGTAGAGCTGCAAGGACACCGCCTGCACAGGATGCCCGGGGCTGGGCATT27080.11299373735966015No Hit
CCACTACGGTGACACCAACCCCAACACCCACCGGCACACAGACCCCAACA26920.1123261229587168No Hit
AGGCAACAAACCTCAAGGTCCCCCACCTCCAGGAAAGCCACAAGGACCAC25860.10790317755246719No Hit
GAGATTGGAGAAATAATATGCCATGGTATTACATAAGCTTTGGCTTCTCT25710.1072772890515828No Hit
CTCTTTTGCTTGTCCTTCTTGTTCATGTCAGTGTCCCTGAGCATGACGAT25660.10706865955128801No Hit
CACAGATGGTGGTGTTGACGGTGATGCACACGGGGCAGCCCTCCTTCTCC25550.10660967465063947No Hit
GGTGGAGGTGAGGCAGATGCCCAGCAGGCGGCACACGTGGGGGTTGTCCA24980.10423129834727883No Hit
CACGTCCGTCGAGAAGATCTGGACAAGCTCCACAGAGCTGCCTGGTGGGG24900.10389749114680716No Hit
GGCTCACTCACCAGAGATGCGGCCTGCCACGTGCACTCCCATGGTGACTC24620.10272916594515631No Hit
TCCTTGGGTCCATCGTGTAGCACCAGGGCCCATGGCTATCCCCATCTGGG24470.10210327744427192No Hit
GGAAGACTCGGAGCAGGTAGGCACGAAGCACACAGGCTTGTTACAGACGG24360.1016442925436234No Hit
GCACTGCTGCTGTTGATAAGACATCCTGCTGGAGTCTCAGAATCTGAAAG24300.10139393714326964No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)