FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001128948

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001128948
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3189954
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATCACTGCCAAGTTAGACAGCCCCAGGGTGACAGTGGCCCAGCTGGAC179000.5611366182709845No Hit
TGGTGATGGGTGTCGATGTTGGGGTCTGTGTGCCGGTGGGTGTTGGGGTT75940.23805985917038303No Hit
GGTCTCTTGCAGTCTGGAAGAGGACGGGCTCGTGGACAGAGGGCGTGGCC67560.21178988787926095No Hit
CGATGACAGCGCCTTCATGGAGCCGAGGTACCACGTCCGTCGAGAAGATC59220.18564531024585307No Hit
TCCTTGGGTCCATCGTGTAGCACCAGGGCCCATGGCTATCCCCATCTGGG53150.1666168226877253No Hit
GGTAGAGCTGCAAGGACACCGCCTGCACAGGGTGCCCGGGGCTGGGCATT51580.16169512162244346No Hit
GCCGTAAAACCTCCAGTGACCCGTTTGCCTTCCCTCCTTCCCCCAGGTGA49820.15617780068301923No Hit
GGAGGATTCTGTGGCGTATGAAGACCACGGGATAACCGTATTGCCCAAGA48410.15175767424859418No Hit
CTGTGATGATTTGGCTCCTGTGGCAAGACAGCTTGCTCCCAGGGAGAAGC44140.13837190128760476No Hit
GCTGAAATTGAACTCTGCTGTTGCTGTTGCTGTTGCTGTTGCTGCTGCTG44070.13815246238660495No Hit
AAGCATTCAAGTAGCAGGTATTTCCCATATTCTGGAGCCCAGCCCCCACC44050.13808976555774785No Hit
CCATGGCTATCCCCATCTGGGTCTGGCAGAAGTTCTCCTCCAGTTGTGCA43760.13718066153932001No Hit
ACAAGGAGGCAACAAACCTCAAGGTCCCCCACCTCCAGGAAAGCCACAAG43270.13564458923232123No Hit
GATATGCTTTATGGTCTGCTCAGTCACCTCGTTCTTAATCCCTTTCTCAA43000.13479818204275046No Hit
GACTCTTATCCATCCGTCCTCTCCCAGCTCACCAATCACTCGGCCTAGGC42900.13448469789846498No Hit
GGGAGCGTCACGTCTACCCGCCAGTTCCAGTCATAGCTGACTTGCAGTCC42650.1337009875377513No Hit
GCATGCACCCTCCTAACTGAGGTCTGGTGGGAGCCATGGAAGGACCAGGG42120.13203952157303836No Hit
GTGAAGACCAAGAACATGAAGATGATGGTGAAGATGAAGATGATGAAGAT40690.1275566983097562No Hit
GGAAGACTCGGAGCAGGTAGGCACGAAGCACACAGGCTTGTTACAGACGG40130.12580118710175758No Hit
CCACTACGGTGACACCAACCCCAACACCCACCGGCACACAGACCCCAACA39920.1251428703987581No Hit
AAGGTGCTGACTTCCTTGGGAGATGCCATAAAGCACCTGGATGATCTCAA39630.12423376638033025No Hit
CTGTGGATGCTGAGGAAGTGTCGGTGACAGGAAGAGGGGTGGTGTCACCT39330.12329331394747385No Hit
GGTGAAGCAGTGTCCTTGTCCCAGGGGCTGCTGCTGTTGCTGCTGCTGAG39080.12250960358676018No Hit
CTCTTTTGCTTGTCCTTCTTGTTCATGTCAGTGTCCCTGAGCATGACGAT38750.12147510591061815No Hit
GGTAGAGCTGCAAGGACACCGCCTGCACAGGATGCCCGGGGCTGGGCATT38580.12094218286533284No Hit
CAACTTCTACCACCAACATCACCCCCAACCACACCAGTACAGGCACCAGA38410.12040925982004756No Hit
CCTGATTCCTGTCCTCAGCTACCTGGCCAATGACATGGAGGAGGACGACG37920.11887318751304878No Hit
GTGCTCTTCAATGACTCAACACGCCTCATCCTCTACAATGATGGTGACAG37680.11812082556676366No Hit
CCTGATTCCTGTCCTCAGCTATCTGGCCAATGACATGGAGGAGGACGACG37480.11749385727819273No Hit
GAGATTGGAGAAATAATATGCCATGGTATTACATAAGCTTTGGCTTCTCT37140.11642801118762214No Hit
CACACTCGTGCAAGGCTTAGAAGGCCAGAAGATCACACGCGTGGCTTGTG36330.11388878961890987No Hit
CGCCATGTTCTCAGATAAGAAGTACCTGACCGCCAGCCAGCTTGTTCCCC36250.11363800230348149No Hit
GAACCAGCAGATGCCTTCTGTTTTGCCTTCCCAGAACAAGCCTTCTCTCC36090.11313642767262476No Hit
GACTCTTATCCATCCGTCCTCTCCCAGCTCACCAATCACTTGGCCTAGGC36070.11307373084376766No Hit
GCCCCACCAAGCCAGATAGGAAGGCCACTGTTGGGAGGCAGGAGGGAAAG35810.11225867206862544No Hit
GTCTAAGATAATATCGCTTCTGATTCTCTTCTTCTCCATCTTCTTCATCT35650.11175709743776868No Hit
GCACAGGCTATGAGAAGTACAACTCCATGCGAGCTGACCCCGCGCTGTGC35590.11156900695119742No Hit
CCAATACACATTACTGACATGTATGCCCACCTGACCTGCACCCACTCATG35590.11156900695119742No Hit
CTTGTCTCCCCAGATGTCAAGAATATGAACACGTGGCTGCTGTTCCTCCC35420.11103608390591212No Hit
AATGTCCTTCTTACCCGTCACCACCATCTGCTAGCATGTCTGTTCCTCTT35280.11059720610391247No Hit
CCCCACGATGTCAGAAGGTGCCACAGTCTCATGGAGCATATAATACTGCA35200.11034641878848409No Hit
CATACACCCTCATCCTGCATCTCATCCACAGGATGAATTCTGCCGCCGGC35120.11009563147305573No Hit
GGCACTCACCTTCTTCATCAAGGAGTTGGGATGGGAACTCACGGTGAGGT35060.10990754098648445No Hit
GGTAGAGCTGCAAGGACACCACCTGCACAGGATGCCTGGGGCTGGCCATT34800.10909248221134223No Hit
GCTGGTTGCCTCCTTGTGGGGGTGGTCCTTGTGGCTTTCCTGGAGGAGAT34740.10890439172477094No Hit
GCCGTTGTTCGAGCAGTACATCAACAACCTCAGGAGGCAGCTGGACAGCA34390.10780719721977183No Hit
GAGTCGCACAGCTCAGCCTGTCCCTGACATGTCTCTGTGCTTTGTAGGCG33750.10580089869634483No Hit
GGTAGAGCTGCAAGGACACCACCTGCACAGGGTGCCCGGGGCTGGGCATT33550.1051739304077739No Hit
CTGTGGATGCTGAGGAAGTGTCGGTGACAGGAAGAGGGGTGGTGTGACCT33260.10426482638934605No Hit
CATGCGTCTTCACCTGGAAGGGGTCCATGTGCCCCTCCTTCTGGCCACCA33220.10413943273163187No Hit
CCACTACGGTGACACCAACCCCAACACCCACCGGCACACAGACCCCAACC33180.10401403907391768No Hit
CCTCTCCAGATCCCATGTGAATGACAGCCAGAATAACTCGAGCATAGGAA33150.10391999383063204No Hit
AGGGTCACAGATCTTTAACAAGAATGGGGGCACTCAGGGTCTGAGGCCAC33130.10385729700177494No Hit
GCCAGATCAACACCCTCAACCACGGGGAGGTGGTGTGCGCTGTGACCATC32230.10103593970320576No Hit
CAGAGCCTGGAAATGGTTGAGACTACCCTGCCACGATTTTGCTCCCGCTC32220.10100459128877722No Hit
GTTCTCTGTTGCATGCTTCTCCTTCTCCACCTTGGCCAGTGTCAGCTCCA31940.1001268356847779No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)