FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001128995

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001128995
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCCCCCACCCCTCGACGCTTCTCACCCACCTGAAAAACATACGAATA11.639344262295082No Hit
ACCATAAGACTCCTAGTAGATTCACAAATGTTTCGGGCAGACCAAGCCAG11.639344262295082No Hit
CAAAGTGCTCACAGTGTGGCATGGATCAGCTGAGAAGGGCGGATGCGCTT11.639344262295082No Hit
ATTTACCTGACGTTGCATTGAATGCGCTGAGCATCTTGTGTGTCCCTGTC11.639344262295082No Hit
TGTTCTGCTCATCTGTTTCATGCTCCACAAATTAACTTCTCCAGTTTATT11.639344262295082No Hit
AAGGTAATGGGCTAGAAGGCAGAAGGGTGAGTCACCTACAAAGTCACCAG11.639344262295082No Hit
AGCCTCCAGGATATTACGTGTGCACCGGCGTGGGATCCTGGAACTGGCAG11.639344262295082No Hit
TTGAGACAGGGTCTCACTATTGCCCAGGCTGGAGTGCAATAACACGATCA11.639344262295082No Hit
TTCTCTTCCTGTTCCTGCGTCATCTCCTCCTGCTCTCGTATCTTCTCCTC11.639344262295082No Hit
TATAACCTAAGAGTAAGAGTTTTTGGTTAGAAGTTGAGACAGAGAAGATT11.639344262295082No Hit
GATCTTCACCTCTAGCTCGCCGTTGGCCGCCTCCAGGGCGCGCACCTTGT11.639344262295082No Hit
AAAAGCTCTACAAAGAAAATGAAGGAAAGCCAGAGAATGAAAGAAACCTA11.639344262295082No Hit
CACAGTGACTGGCCCCCCCCCAGTGACCGGCCCCCCCCGCAGCGCCCAGC11.639344262295082No Hit
AGACCAGTGGCTCTCCAAGCACTAAGCCAGCAGCCACATCAGAATCCCAC11.639344262295082No Hit
AGGCTATCAGAGCGGGCATTGAAGGCCCATGATGCTCATTCTCCTCTTCC11.639344262295082No Hit
TCAGCAGCAGCAAGAAAGGTGGCTTTCTGTGGCCAGAAGGCGCAGCAGCA11.639344262295082No Hit
GTTGGGACCAGAATTATTGCCTGTTTCCTCCCCAGGGCTGGTTCAGGAAC11.639344262295082No Hit
TTTCTAGCAGTAGGATGGGTTCAGGTCAGAAATGGGAGAGCATTCAGAAT11.639344262295082No Hit
AGCCTCTCATAAGCCTGGGGTTTTGGGTCATCAGGGCCTATGGCCACCTT11.639344262295082No Hit
GCTCTCAGATAGTCCTGTTCCAGAAACGACGGTTCCACTTCTTCTGTTCC11.639344262295082No Hit
AAACCTTCCGACCCCTCTCCGGAGTCCGGTCCCGTTTGCTGTCTCGTCTG11.639344262295082No Hit
TCAGCATTGAAGTCAGGCTGCAGCAGAACCTCAGCCTGAGCCGTGGGCGC11.639344262295082No Hit
GGCCGGTGCCGGAGGCCGCGGTCGCGCACCCCCGGGCCAGCGTAGGCGGG11.639344262295082No Hit
CGCCCTGTGATTCTGGGCTTGCATCTGTACACAAAGTAAAGGAAGCAAAA11.639344262295082No Hit
GAAGAAGGAAGCTTCCCTGCCTGAGACACATATGGAAGCCAAGAGCTCCA11.639344262295082No Hit
CGTGCCCTTCTCGGGCTCCAAAAATCAGCAGTGCTCCGGGCGCCCCCTGC11.639344262295082No Hit
CTCGTCCTCCTTCCTGCTGTTGAAGATGCCCAGGCTGAATGAGTTGGGCT11.639344262295082No Hit
CAATTCTTTCTCTTCATCCGGTAGCAGTCCACACATACTCCAAACCCACA11.639344262295082No Hit
AGTGCTCCTCCCCTGGTGCTCATCTGGGGCCTCAGGGCTCCTCCCCCGGT11.639344262295082No Hit
TCCCCATCAAGTGGACAGNNNNTGAAGCCATCAACTTTGNCTCCTTCACC11.639344262295082No Hit
CTGCAGAGTGCCCGTGACTGGCTCTGTCTTCTTGATGGAACCCAGGGTGT11.639344262295082No Hit
CTGGGGAGGACACAACCAGGTAGAGAGGACCCCTACCCACTAGTCCCTGC11.639344262295082No Hit
TTCTCCCCGTTGACGTAGCTGGCATCTGGAGAGCACAGGAAGGACACGAT11.639344262295082No Hit
TGAGTGTCACGCCTTCTCCTCTCTGTCCCCAGCATGGGCACCAGCCTCAG11.639344262295082No Hit
TGGGCGGGCCCGGAGGTGATCCCAGGCTGCGGAGGCTGGGGACCCGGTCG11.639344262295082No Hit
CAAACCACAATAAACTTGACTCTTAATTTTAACTTATGTCACAGAGCTGC11.639344262295082No Hit
GGTCTAAGTAGTGCATTCAATCAGAGATGATAGCGAAAATATTTAACAAT11.639344262295082No Hit
GATTTCGAAGATCCGTGTCTTTGGCTTTAATTCAGCGCGTAGTTTCTGTC11.639344262295082No Hit
AGCCGGCGGCTCCTCAGTCGACCCCAGGACGCGCTGGAGGGTGTTGTGCT11.639344262295082No Hit
ACCAGGGGCACCGAGCGATCGCATCCTTCTGAACCAAGGTGAAAATAAGG11.639344262295082No Hit
CTTCTCCTGCTGCTGCACATGATGCATGTACTCCATCACCCCAATCCTCA11.639344262295082No Hit
CTGTGCTGGGCGCTTGGGGAACCTCACCTGGAGCCCTGTGTCTCCTGCTC11.639344262295082No Hit
CAGCGAGAGTAGGTGGTAGGTAGGCAATGGCCAGTGGGGCCAGCGCGGGT11.639344262295082No Hit
GCATCGTGAACAGCCTGGCAGGAGGAAGGCCTGAGGAGGCAGTCCCCACT11.639344262295082No Hit
CTGACTTCAAGTCCCTGCCCTGCCATCCTGGCTGTGGGGACTTGGACAGG11.639344262295082No Hit
AGGTGTTTCCCTGGGGGTGCCCAGAGTGGAGGTGGTAGCTCAGGTACCTC11.639344262295082No Hit
AGGGAAGGTCACCATCAAGTATGACCCCAAGGAGCTACGGAAGCACCTCA11.639344262295082No Hit
TGAAAAGAAAAAGCAAGAGAACAAAATGAGAAGAGACCAGTTGAACGACC11.639344262295082No Hit
CCCTGATGGCACAGTGGCAGGCATCTGGGTCCTTGGGGAACTCCCCATGA11.639344262295082No Hit
TTGGAGTAGAGGAGTAGATGATGTGATCTTAAGGTTCCTTCCAGCTAGAA11.639344262295082No Hit
CCTGTGGACCGAATGGCTTGGATGGTGAGATTTCATTTTGTGGATGAGAT11.639344262295082No Hit
TTGGCCTTCTCCCCAAAACAGGCCCTGTCGACTTCCTTTCTGAGGACGTT11.639344262295082No Hit
ATCTTCCGCGTGCTGGTGTACCTGGTGACGGCCGAGCGTGTGTGGAGTGA11.639344262295082No Hit
TGCCTGGCTGCGGAAGTGCCCGGGCTGGCACACGCAGCCCGAGGCTCGAG11.639344262295082No Hit
ACCTCGGCGTCGCGGTTGAGGCGGTACACGTAGAGCTGCGAGGTCCCGGC11.639344262295082No Hit
ATTTCTGGATGGCCAAAGTACAATAATATCTGCTTATTGTGAGAGTATTT11.639344262295082No Hit
TGAGACTCCTTCTCAAAAGAAAAAAAAGAAAATGATTGAGAGACTCAAAG11.639344262295082No Hit
TTAGCATCAGACAACAGTCAGCGCTTTCGAGAAACCTGTTTTGCATTTGC11.639344262295082No Hit
TCTCCAGATTGAAACTTCTCACTCTTCACCATCCAGTTGTCCAGCCGCAG11.639344262295082No Hit
TCATGATGCTTGCCTTGTCCAGAGACCTTTCCTTTTGGAGCTTTTCTCCC11.639344262295082No Hit
ATTGGGGTTATTTGTGCCAGAGGCTAATGACCCATGCTGGCCCTTCACTT11.639344262295082No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)