FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001129226

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001129226
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2549798
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGTCCTTCCATGGTGACTCTGGCTCTTGCAGGAGGTGGGCTACTGCAG223250.875559554129386No Hit
AGTGTGCCTTGGCTGAAAGCTGTGATCACCTGGCCAGGGTGGCCATCCCC176020.6903291947048354No Hit
AGCCACTATCAAATGCTGTGAAGAGAAATGTACCCAGATGTATCATTATC141040.5531418567274741No Hit
GCTTTGTTGGCCTCAAATAGCTGCTTTATTAGATGCAATGAAGCAGGGGA117550.4610169119279253No Hit
GCTTTGTTGGCCTCAAATAGCTGCTTTATTAGATGTAATGAAGCAGGGGA113510.44517251954860737No Hit
GTATTTCATTATGTCCTGAGGAACAGTTGGTTATAGAAAGGCTACAAGGA80450.31551518983072385No Hit
CATGCGTCTTCACCTGGAAGGGGTCCATGTGCCCCTCCTTCTGGCCACCA75700.29688626314711986No Hit
ACTTTCACCCAAACCTCCTACACCCTGTTCGTCCGCGAGAACAACAGCCC75690.29684704435410175No Hit
AGGCCCTCATGAATGACCTCTTTCTTTCCTTTCTTCTTTTCTAGCTACTC67400.26433466494208563No Hit
GCAGGTACCCTCAACTGAGTAAGGGACAATGAACAGCCTCTTGGCCATTC66000.25884403391954974No Hit
AGTAAATACATTGGTTGAAGTTTAAAAGTCTATCCGTAGTAAACTTACAT63010.24711761480713376No Hit
GGAAAATGCTGGCTGACCTAAAGCCACCTCCTTACTTTGCCTCCTTCTGC60290.23645010310620684No Hit
AGTAAATACATTGGTTGAAGTTTAAAAGTCTATGCGTAGTAAACTTACAT59790.23448916345530116No Hit
GCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTGGATCCCAG55940.21938992814332742No Hit
GGTGGAGGTGAGGCAGATGCCCAGCAGGCGGCACACGTGGGGGTTGTCCA55700.2184486771108927No Hit
CATCATGATCAACCAGTGAAGCCCCTGGATCGAGCAGTCTTCTGGATTGA55410.21731133211336742No Hit
AACAGAGAGAATTTTCACTATGGATCAGTGGTGACCTACCGCTGCAATCC55180.2164092998739508No Hit
CACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTTTCGGAG54460.2135855467766466No Hit
GGCATACAGTGGACGAGAAACAGTATATTCCAATGCATCCCTGCTGATCC53410.20946757350974468No Hit
TTGCCTCCTTGGTATGAAACATCTTTCACACAACCCTCAATTGTAGGAGT53350.20923226075163603No Hit
CCTGAAGTCTGGCTATGGAAAATATCTTGGTATAAATTCAGATGAACTTG48080.18856395683109015No Hit
GTCAAAGGAGTGTTGGAGTCAACAGGAGTTGTTGAAAGGGTGCTAGCCTC48030.18836786286599957No Hit
GCGGGACCACAATCCTTCGCTTCTCTCCAATGCAAACACCAAGTAGACCT47260.18534801580360483No Hit
GCCCACAGCATCCTTACCTGCTGTGTGACTCGTTCTGGGTACCATCTCCC45950.18021035391823195No Hit
AAGGCACACTTACCACTGTTTTCTTGGCTCCAGTCCAAACCTGAGAGTAG45620.17891613374863422No Hit
CAGGAACGTACTGGTGAAAACACCGCAGCATGTCAAGATCACAGATTTTG45490.17840628943939874No Hit
GGTTTGAAGACCACTTAAAAGCTTTGCCACATTCTTGACATTTGTAGGGT44060.17279800203780848No Hit
TGCTGTAACAAAGGTGACTGTGGCACTCCAAGCCATTCCAGGAGGCAGCC43850.1719744073844281No Hit
GGATGACCTCATGCTCATCAAGCTGGCTAAGCCTGCCATGCTCAATCCCA42840.16801330928959862No Hit
GAGAAATTATTTAATACTTCAGCCAAGAAGTCTTTCTAGACTAAATTGTT42640.16722893342923636No Hit
GTAGGTGTCTTCTCCTCGATCCCTGGGATGGGAGCCTTGTGGGGTAGTAT42490.16664065153396465No Hit
TGTGTGAATGCAGTCATCTGTATAAAATGTCATCAATACCTTGATCATTT42270.16577783808756616No Hit
GGAGGAAGAAGATCTGCACACCACAGCCAGCGCGGGAGATGGCCTTATTT41370.16224814671593593No Hit
ATCCTGGCTCAGCAACCTACCCTTTTCTCCCTGGGACTCCTTGTCTGCTG41270.1618559587857548No Hit
AGGTGACACCATGCCTCTTCCTGTCACTAGCCCTTCCTCAGCATCCACAG40930.16052251982313892No Hit
AGGAGTCATGGGAGAAAACAACACCCTGGTCTGGAAGTACGCAGACGCCG40720.15969892516975853No Hit
CTGACTATCACAGCGAGTACCACCAAGGTGATCACCACCCCAGTGAGTAC40280.15797329827696155No Hit
CAGACCCATGCTGACCATAGCTGGAAGATGAACCTGAGCTAGATCCGTGT39050.15314938673573358No Hit
GTGAACAGCATAATCGCGTATTTCGTAAAGTCTGCTCCAAAGATGGCCTC38510.15103157191275546No Hit
CCAGGAAGCCTACGTGATGGCCAGCGTGGACAACCCCCACGTGTGCCGCC38340.1503648524314475No Hit
AGTGTGCCTTGGCTGAAAGCTGTGATCACCCGGCCAGGGTGGCCATCCCC37960.1488745382967592No Hit
CTGAGGAGCAGTAAGGGATGGCATCAGCAACGCGAGCCACAGGCAGGCCT35820.14048171659088288No Hit
CCTGCTGTGGGATGAGGTACTCGTCGGCATCCACCACGTCGTCCATGTCT34140.1338929593638398No Hit
CAAGAGAGGAAATGTTCTGTTCTCCTTCACTTTCCACTCACCCGTAGGTG34090.13369686539874925No Hit
CCATTCTAATAGCCTCAAAATCTCTGCACCAGGGGGATGAAAGAATGCAT33180.1301279552341009No Hit
TGGAGCCAGACCATAAATACAAGATGAACCTGTACGGCTTCCACGGTGGC33040.1295788921318473No Hit
TTACAGTCAGCATGGATCTGGCTCAGGTCACTCCTCTGGCTACGGACAAC32850.12883373506450316No Hit
GGTTGATATGGGAGTAAAGACCTTGGCATGGTACATACCCCTCATGCAAG32840.12879451627148503No Hit
CAGACCCATGCTGACCATAGCGGGAAGACGAACCTGAGCTAGATCCGTGT32680.1281670155831952No Hit
CAGGATTCCCTCTACCTACCTTCCCCCCATTTAGGCGTGCATTAGGCCTC31400.12314701007687667No Hit
GTACTGGGAGCCAATATTGTCTTTGTGTTCCCGGACATAGTCCAGGAGGC31210.12240185300953252No Hit
AAAGCAGATCTCAATTAAGCAGACCAGCATTTGCACAGGATCACTCAGGC31000.12157825835615213No Hit
GCCTTGAGCAGCTGGTACGACAGCCAGGCGTTCTGGCCCGAGTCGCCGTC30830.12091153887484421No Hit
GACCTGCGCTGTGAACTGCCCAAGCTGGAGAAGCGGCTGCGTGCCACGGC30550.11981341267033703No Hit
CTCCATCATAGGACCCAAAAGGGGGGAACATGCCTGGACCCACAGACTGC30290.11879372405186608No Hit
GGATACTCATTCTACCCAGTGTCACGAGAAATGAAACAGGACCCTATCAA30290.11879372405186608No Hit
GTAGTCGCCATGGAGGTAACTGGAATGATACGCGCCGTGGTGGTCTTGGT30160.11828387974263058No Hit
TGAAAAGCAAGGCTGTATAGAAGACGGTGACCACGGTGAGGTGAGAGGCG29800.1168720031939785No Hit
CGCCCCTCAGTGCATTATACCTAACAAATGCACGCCTCCAAATGTGGAAA29750.11667590922888793No Hit
CTGGAGAACAATGACCAGGTATTGGGAGGGCTGGTCTCAACACATTTCAC29520.11577387698947132No Hit
CTGCTGTCCAGACCATATTGACTTTCCATTTGCCCTACTGTGGACCCAAC29420.1153816890592902No Hit
CTCAGTCCATTGCCCCAAGGCTCACTTGTTAGCTTGCCTGGGGAAAGGGG29370.11518559509419962No Hit
TGGAGGCGTGCATTTGTTAGGTATAATGCACTGAGGGGCGGGGCCGCTCC29250.11471496957798225No Hit
GAGTCTCAAAGCCATGTTATTCTGCCTTTTTAAACTATCATCCTGTAATC29230.11463653199194604No Hit
GCAGAAGAGAAGGGATCCATCCTAAGCATGACTCCTGAGCAGCTGGGCTC29010.11377371854554752No Hit
CCGCATTAATATCCCAGAGAGAGCAGGTGGACCAGAAGGCAACTTAAACC28990.1136952809595113No Hit
CACAACCAGGAAGCTTATTGGACTGTCCTCAGAGATCTGCACCCTATAGA28950.11353840578743886No Hit
ACGTCAGCCCCAGGAAATGCCCGGTTTGGCCAGGGTTCAGGACCCATTGT28810.11298934268518526No Hit
GATCCACCTCCTGCTGGTAGCGCTTACGGTCCCGCATGGCGTTCTCCTTG28720.11263637354802224No Hit
GGAGGAAGAAGATCTGCACACCACAGCCAGCGCGGGAGATGGCCTTACTT28630.11228340441085921No Hit
CTTGGTCTTCACTGCTGTATGTTGACTTTTTCACTGTTAATTCTAAGGCT28580.11208731044576865No Hit
GAGGTAGAGAAGCTTGGCATCCCAGCCAGCTGAGTAGCGAGTACGGGGGT28350.11118527820635203No Hit
CACAATGTTAACAAAACTCCCTATTTTCCCCACCCCATAGTAGCTCAGCA28340.11114605941333391No Hit
GGCGAGTGTACCAGTACCTGGCTGCCGACTGCACCTCCACCTTCCCTCGG28170.110479339932026No Hit
GAACACCCCACACATTAGGGCGGCTGCCTTGTCCTTCTGGGAGGAGCTGG27840.10918511976242824No Hit
AACAGAGAGAATTTTCACTATGGATCAGTGGTGACCTACCGCTGCAATCT27750.10883215062526522No Hit
GTCTCACACCCATCTCTCCTCTGCCCTCCAGCTCCCCCGCCTCCAAAGCG27320.10714574252548632No Hit
CACAATGTTAACAAAACTCCCTATTTTCCGCACCCCATAGTAGCTCAGCA27310.10710652373246822No Hit
GGTTTTATCCTCAGCTTCTTCATAGCTGTCTTCATATCAGCATTTCTGAG27160.10651824183719652No Hit
ATACAACACTAATGACCCTCCACGTTGGTTTGGCTATGATAGGGAAGGAA27150.1064790230441784No Hit
TGTGTGAATGCAGTCATCTGTATAAAATGTCATCATTACCTTCATCATTT27000.1058907411489067No Hit
CCGGCATGGTGACTGTACAGGGCTGGGCCGGGTGTGCGACTTACCGAGCT26880.10542011563268933No Hit
GCAACTTGCATACCAGTGTTTCCTCAGCACAGGGCCAGGATGCAGCCCAG26650.10451808339327273No Hit
CTGTCCTAATTCCCCACTCCTCTAGGCAGGGCCAGCATTTCTGCGGGGGG26260.10298855046556629No Hit
CTGCGATTTTTCTGCATCAGGCCCAGCAGCACTGTACAGCAGGGTTCACC26030.10208651822614968No Hit
GGGAGTGCAAGTGTCATGGGAGTGCTGGAGGCCGCAGTCATACTGGAGGC25900.10157667391691422No Hit
GTTGCTGCTTCAGGAATGGAGGGAGTGAGAGCCTTTTCCGACAATGCCGA25890.10153745512389609No Hit
CGGATAAAATGCACCCAACTGCATCCACCGCCTACAGAGCTCCTCAGGGG25620.10047854771240702No Hit
CCAGGTCTATATGAGAAACCTTCTCTCTCAGCCCAGCCGGGCCCCACGGT25590.10036089133335269No Hit
GGCCTCCAGTATGACTGTGGCCTCCAGTGCTCCCACGACTGCAGCCTCCA25510.10004714098920776No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)