FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001129268

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001129268
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2201957
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGTCCTTCCATGGTGACTCTGGCTCTTGCAGGAGGTGGGCTACTGCAG142300.646243319011225No Hit
GCTTTGTTGGCCTCAAATAGCTGCTTTATTAGATGTAATGAAGCAGGGGA127230.5778041987195935No Hit
AGCCACTATCAAATGCTGTGAAGAGAAATGTACCCAGATGTATCATTATC126040.5723999151663725No Hit
GCTTTGTTGGCCTCAAATAGCTGCTTTATTAGATGCAATGAAGCAGGGGA122910.5581852869969759No Hit
AGTGTGCCTTGGCTGAAAGCTGTGATCACCTGGCCAGGGTGGCCATCCCC115180.523080150974792No Hit
CATGCGTCTTCACCTGGAAGGGGTCCATGTGCCCCTCCTTCTGGCCACCA62290.2828847248152439No Hit
GGCATACAGTGGACGAGAAACAGTATATTCCAATGCATCCCTGCTGATCC62130.28215809845514694No Hit
AGGCCCTCATGAATGACCTCTTTCTTTCCTTTCTTCTTTTCTAGCTACTC60650.2754368046242502No Hit
AACAGAGAGAATTTTCACTATGGATCAGTGGTGACCTACCGCTGCAATCC60560.27502807729669565No Hit
GGAGGAAGAAGATCTGCACACCACAGCCAGCGCGGGAGATGGCCTTATTT59360.26957837959596853No Hit
AGTAAATACATTGGTTGAAGTTTAAAAGTCTATCCGTAGTAAACTTACAT55290.2510948215610023No Hit
CTGACTATCACAGCGAGTACCACCAAGGTGATCACCACCCCAGTGAGTAC52610.23892383002937842No Hit
GGTGGAGGTGAGGCAGATGCCCAGCAGGCGGCACACGTGGGGGTTGTCCA51340.23315623329610885No Hit
GTCAAAGGAGTGTTGGAGTCAACAGGAGTTGTTGAAAGGGTGCTAGCCTC50020.22716156582530903No Hit
GCCCACAGCATCCTTACCTGCTGTGTGACTCGTTCTGGGTACCATCTCCC49350.22411881794240307No Hit
GGTTTGAAGACCACTTAAAAGCTTTGCCACATTCTTGACATTTGTAGGGT47410.21530847332622755No Hit
AGTAAATACATTGGTTGAAGTTTAAAAGTCTATGCGTAGTAAACTTACAT46280.21017667465804282No Hit
GTAGGTGTCTTCTCCTCGATCCCTGGGATGGGAGCCTTGTGGGGTAGTAT44810.20350079497465212No Hit
TGTGTGAATGCAGTCATCTGTATAAAATGTCATCAATACCTTGATCATTT44760.20327372423712178No Hit
GGAAAATGCTGGCTGACCTAAAGCCACCTCCTTACTTTGCCTCCTTCTGC44600.20254709787702485No Hit
GTATTTCATTATGTCCTGAGGAACAGTTGGTTATAGAAAGGCTACAAGGA42610.19350968252331902No Hit
CTGCTGTCCAGACCATATTGACTTTCCATTTGCCCTACTGTGGACCCAAC42590.1934188542283069No Hit
GGAGGAAGAAGATCTGCACACCACAGCCAGCGCGGGAGATGGCCTTACTT40990.1861525906273374No Hit
CACAACCAGGAAGCTTATTGGACTGTCCTCAGAGATCTGCACCCTATAGA39620.17993085241900728No Hit
GTAGTCGCCATGGAGGTAACTGGAATGATACGCGCCGTGGTGGTCTTGGT39550.17961295338646485No Hit
GACCTGCGCTGTGAACTGCCCAAGCTGGAGAAGCGGCTGCGTGCCACGGC39450.17915881191140426No Hit
GATCCACCTCCTGCTGGTAGCGCTTACGGTCCCGCATGGCGTTCTCCTTG38050.17280083126055595No Hit
GAACACCCCACACATTAGGGCGGCTGCCTTGTCCTTCTGGGAGGAGCTGG35860.16285513295672896No Hit
GGATACTCATTCTACCCAGTGTCACGAGAAATGAAACAGGACCCTATCAA35150.15963072848379872No Hit
CGAGTGCGATGACCAGAGCGACGAGGAGGGCTGCCCCGTGTGCTCCGCCG34830.15817747576360483No Hit
CAACCCTGCATCTTTAGACAAGGCCATGTCCATTCCCATGTGGGTGGACA34480.15658798060089274No Hit
CTCAGTCCATTGCCCCAAGGCTCACTTGTTAGCTTGCCTGGGGAAAGGGG34350.15599759668331398No Hit
GCAGGTACCCTCAACTGAGTAAGGGACAATGAACAGCCTCTTGGCCATTC34350.15599759668331398No Hit
GTCACATGTGATCCAGGTCTCCTCCGAGGGGCTGGCCAGTCTCTTCTCCC34270.1556342835032655No Hit
GCCCCAAGCTCGTATCCCGGAGGGCAGGGCCAGGGTCTTGGCAGAGCCTT34120.15495307129067462No Hit
AAAGCAGATCTCAATTAAGCAGACCAGCATTTGCACAGGATCACTCAGGC34050.1546351722581322No Hit
GCGGGACCACAATCCTTCGCTTCTCTCCAATGCAAACACCAAGTAGACCT33410.1517286668177444No Hit
CAGGAACGTACTGGTGAAAACACCGCAGCATGTCAAGATCACAGATTTTG33370.15154701022772016No Hit
AACAGAGAGAATTTTCACTATGGATCAGTGGTGACCTACCGCTGCAATCT33060.15013917165503232No Hit
TGGAGGCGTGCATTTGTTAGGTATAATGCACTGAGGGGCGGGGCCGCTCC33030.15000292921251415No Hit
GGTTTTCTGCACTGCTTGTAAAACAGACTTGTAGATGCGGAAACTGATGG32770.1488221613773566No Hit
ACTTTCACCCAAACCTCCTACACCCTGTTCGTCCGCGAGAACAACAGCCC32660.14832260575478995No Hit
GCGGGACCACAATCCTTCGCTTTTCTCCAATGCAAACACCAAGTAGACCT32510.14764139354219905No Hit
CATCATGATCAACCAGTGAAGCCCCTGGATCGAGCAGTCTTCTGGATTGA32140.14596107008447487No Hit
GGTGGCGTGACCTGTAGATGCTGAGGAAGTGTCGGTGACAGGAAGAGGGG31940.14505278713435366No Hit
TGTGTGAATGCAGTCATCTGTATAAAATGTCATCATTACCTTCATCATTT31570.14337246367662948No Hit
GCCACCAAATCGGGAGAAGCGGCGGTCTCCGCTGAAGGTGCTGAGCAGCA30720.13951226113861442No Hit
CGTGTTGTGCAGTCTCTTTCTGTGCAGGTCGTCCTTCCGGACCAGCACCA30430.1381952508609387No Hit
GGTTAGGTAGGAGCTTACTCAGTGCATCTGGAGGATGGAAAACCAATGGC30430.1381952508609387No Hit
CTGTCCTAATTCCCCACTCCTCTAGGCAGGGCCAGCATTTCTGCGGGGGG30160.1369690688782751No Hit
AAGTGGTGACATCAGAACCTGCCCTCCACACCCAACAGCTGGACTGCAGC30150.13692365473076903No Hit
GACGTGTCTAAGGTGGCATTCCTGACCGTGACCCTACACCAAGGCGGAGC30020.13633327081319027No Hit
CATCGTGTTTGGGAGTTTCCTGCTGCTGCTCTTCTCCCTGACCCAGTTCA29560.13424422002791153No Hit
TTGCCTCCTTGGTATGAAACATCTTTCACACAACCCTCAATTGTAGGAGT29540.13415339173289942No Hit
ATGGCATTGTATGAGAGCATGAGGGAACGCAGGTTGGACAGGTGGGCAAA29410.13356300781532066No Hit
GGCAAGTGGAAAGCCTTCTCCACCTGTGGCTTACACCTCACTGTGGTGTC29340.13324510878277823No Hit
GAAATATCCAGTTACTCCTCTAGTCCCATCACCTCGCTTTATGATGTGTA29310.13310886634026003No Hit
GGTTTTATCCTCAGCTTCTTCATAGCTGTCTTCATATCAGCATTTCTGAG27550.12511597637919358No Hit
CAATGTCCACAAAGATGACCATCTCGTTGGCTGAGGTGTCTGCACAGGCC27390.12438935001909666No Hit
GTGTGATCTGAACCCTTTGCTCAACCTCGCCTGCACTGACACCCATATGC27200.12352648121648152No Hit
ACCTTACCTTTTTGTCACTGAGGCCGGTCACTTGGTCCACAAATTCCTCT26770.12157367287372098No Hit
CCTGAAGTCTGGCTATGGAAAATATCTTGGTATAAATTCAGATGAACTTG26500.12034749089105737No Hit
AGTGTGCCTTGGCTGAAAGCTGTGATCACCCGGCCAGGGTGGCCATCCCC26330.11957545038345435No Hit
ACCAGCAAGATCATGGTATCTGTATGGGCCTTCTTCGCTGTCATATTCCT26270.119302965498418No Hit
CATCTAATTTCCCGGGCCATACCCTGCAGGTACTGGATGATGCTGAGGTT26040.11825844010577863No Hit
AAGGCACACTTACCACTGTTTTCTTGGCTCCAGTCCAAACCTGAGAGTAG25670.11657811664805443No Hit
GACAAGCTGGTGTCTGTATTCTACACCATCCTCACTCCCATGCTGAATCC25630.1163964600580302No Hit
GCCGCTGACCAAGAATGAGGGGCTGTGGGAGGCAGGGGCTGGTGAGCAGC25370.11521569222287266No Hit
CCTGCATATAGTGGACGAGAAACAGCATATTCCAATGCATCCCTGCTGAT25370.11521569222287266No Hit
CGACCTTATCCAGCCACACGCCACCATGTGCCACACCAGCTGCTCCTCGG25300.11489779319033024No Hit
GAGTCTCAAAGCCATGTTATTCTGCCTTTTTAAACTATCATCCTGTAATC24970.11339912632263027No Hit
AGGTGACACCATGCCTCTTCCTGTCACTAGCCCTTCCTCAGCATCCACAG24940.11326288388011209No Hit
TGCTGTAACAAAGGTGACTGTGGCACTCCAAGCCATTCCAGGAGGCAGCC24360.11062886332476064No Hit
GGATGACCTCATGCTCATCAAGCTGGCTAAGCCTGCCATGCTCAATCCCA24280.11026555014471218No Hit
AGGAATGACCACAGTTCAGTGCCCTGATGGTGTGACATTCACATTCAGCA23950.10876688327701221No Hit
AAAGTACTCACTCTCCACACTTGCCCTTTCCTAACACTTTCCTGAGTGCT23890.10849439839197587No Hit
GCTGAGTTTGAATGCATCCACCCTGAGAAGCAGCAGAAAAAGAAAAGCTA23890.10849439839197587No Hit
ACCCATTTTGTGACAGATGCTGCCTCTCCCTCAGGCCCTTCACCTTCGTG23860.10835815594945769No Hit
AGAGAAGCTGCTACTCTTTTCACTCGAGCCATGGGGAGAAGCGCTGCCAA23860.10835815594945769No Hit
ATGGTCAGCTCCTGTTGTGGTTCCGTCTGCTCTGACCAGGGCTGTGGCCT23520.10681407493425166No Hit
CCTGGCCTCCTCTTGATGGCACAGGACCTTCCCCATCCCGCCCCTGGAGC23440.10645076175420319No Hit
TGTGTGAATGCAGTCATCTGTATAAAATGTCATCATTACCTTGATCATTT23100.10490668073899717No Hit
GGTGATAGCAGTTGGTGTCATGGAGCTCAGGAATCAGAGCCTTGGTTACA23080.10481585244398504No Hit
AGAGAACCCTTACAAATTCGAAGAATGTGGTAAAGCCTTTTATTGGTTTT23010.10449795341144265No Hit
AACTGGAGGGACATTTGAAGGGGCCTTGAGCAGGAGGGGAGCTGGGTGCA22850.10377132705134569No Hit
GCAGAAGAGAAGGGATCCATCCTAAGCATGACTCCTGAGCAGCTGGGCTC22640.10281762995371843No Hit
GTCTGTTTTTAGACATTGCCATCGGAGTGCCTTTTGCAGGCAAGGATCAA22610.10268138751120028No Hit
AGGAAGATGATGAGGTTCTTGGCGGCTGTCTGTGCAGGCTGCAGCTTCTT22490.10213641774112754No Hit
GGTTTTATCCTCAGCTTCTTCATAGCTGTCTTCATATCAGTATTTCTGAG22170.10068316502093366No Hit
AAACTGACAAAGTCCATCTGGAGGCGGGGGCCGCCGTAGCGGATGAGCAT22060.10018360939836699No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)