FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001129274

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001129274
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2050946
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGTCCTTCCATGGTGACTCTGGCTCTTGCAGGAGGTGGGCTACTGCAG333671.626907778166758No Hit
AGTGTGCCTTGGCTGAAAGCTGTGATCACCTGGCCAGGGTGGCCATCCCC212741.0372774319752933No Hit
AGCCACTATCAAATGCTGTGAAGAGAAATGTACCCAGATGTATCATTATC169420.8260578289238235No Hit
AGTAAATACATTGGTTGAAGTTTAAAAGTCTATCCGTAGTAAACTTACAT91400.4456480082849573No Hit
AGTAAATACATTGGTTGAAGTTTAAAAGTCTATGCGTAGTAAACTTACAT82670.40308228495533277No Hit
AGTGTGCCTTGGCTGAAAGCTGTGATCACCCGGCCAGGGTGGCCATCCCC71190.3471081149869377No Hit
AGGTGACACCATGCCTCTTCCTGTCACTAGCCCTTCCTCAGCATCCACAG70420.343353749928082No Hit
GCTTTGTTGGCCTCAAATAGCTGCTTTATTAGATGCAATGAAGCAGGGGA69130.3370639695048041No Hit
GCTTTGTTGGCCTCAAATAGCTGCTTTATTAGATGTAATGAAGCAGGGGA64780.3158542448216579No Hit
GGAGGAAGAAGATCTGCACACCACAGCCAGCGCGGGAGATGGCCTTATTT63980.3119536057994701No Hit
GTATTTCATTATGTCCTGAGGAACAGTTGGTTATAGAAAGGCTACAAGGA61640.30054423665957075No Hit
GCCCACAGCATCCTTACCTGCTGTGTGACTCGTTCTGGGTACCATCTCCC60730.29610725977183217No Hit
GGTTTGAAGACCACTTAAAAGCTTTGCCACATTCTTGACATTTGTAGGGT58920.2872820639841322No Hit
GGCATACAGTGGACGAGAAACAGTATATTCCAATGCATCCCTGCTGATCC57300.2793832699642019No Hit
CTCAGTCCATTGCCCCAAGGCTCACTTGTTAGCTTGCCTGGGGAAAGGGG55120.2687540286287401No Hit
GTAGGTGTCTTCTCCTCGATCCCTGGGATGGGAGCCTTGTGGGGTAGTAT53800.2623179742421302No Hit
TTGCCTCCTTGGTATGAAACATCTTTCACACAACCCTCAATTGTAGGAGT48250.23525729102570228No Hit
GTCAAAGGAGTGTTGGAGTCAACAGGAGTTGTTGAAAGGGTGCTAGCCTC39010.19020491031943307No Hit
GTTGCTGCTTCAGGAATGGAGGGAGTGAGAGCCTTTTCCGACAATGCCGA38810.18922975056388613No Hit
GGAGAACCAGAAAATGATGAACCAAGATGCCAGAACCAGGAGGCCACAGA38670.18854713873500326No Hit
CTGCTGTCCAGACCATATTGACTTTCCATTTGCCCTACTGTGGACCCAAC38660.1884983807472259No Hit
CATCATGATCAACCAGTGAAGCCCCTGGATCGAGCAGTCTTCTGGATTGA37800.184305193798374No Hit
AACAGAGAGAATTTTCACTATGGATCAGTGGTGACCTACCGCTGCAATCC37540.18303748611616297No Hit
CTGTCCTAATTCCCCACTCCTCTAGGCAGGGCCAGCATTTCTGCGGGGGG36860.17972194294730334No Hit
ACTTTCACCCAAACCTCCTACACCCTGTTCGTCCGCGAGAACAACAGCCC36340.17718652758288125No Hit
GGTTGATATGGGAGTAAAGACCTTGGCATGGTACATACCCCTCATGCAAG36160.176308883802889No Hit
GTGTGATCTGAACCCTTTGCTCAACCTCGCCTGCACTGACACCCATATGC33950.1655333685040952No Hit
GGCAGTTCCACCATCAAGTACACCACCACCTCCTCCTCCAGCAGGAAGAG33040.16109639161635655No Hit
GGATACTCATTCTACCCAGTGTCACGAGAAATGAAACAGGACCCTATCAA32980.16080384368969247No Hit
AGTGTGCCTTGGCTGAAAGCTGTGATCACCTGGTCAGGGTGGCCATCCCC30170.14710284912425778No Hit
CGCCCCTCAGTGCATTATACCTAACAAATGCACGCCTCCAAATGTGGAAA29710.14485998168649977No Hit
GGTTGTCTACCTGAGCTACATGGCGGTGGAGTACAATGTGTCCTTCCCCC29280.14276338821207385No Hit
CTCCATCATAGGACCCAAAAGGGGGGAACATGCCTGGACCCACAGACTGC28680.139837908945433No Hit
AAGGCACACTTACCACTGTTTTCTTGGCTCCAGTCCAAACCTGAGAGTAG28510.13900902315321806No Hit
GGATGACCTCATGCTCATCAAGCTGGCTAAGCCTGCCATGCTCAATCCCA28430.1386189592509993No Hit
CTGACTATCACAGCGAGTACCACCAAGGTGATCACCACCCCAGTGAGTAC28360.13827765333655787No Hit
TGTGTGAATGCAGTCATCTGTATAAAATGTCATCAATACCTTGATCATTT28330.1381313793732258No Hit
GGCAAGTGGAAAGCCTTCTCCACCTGTGGCTTACACCTCACTGTGGTGTC27750.13530341608213964No Hit
GAACACCCCACACATTAGGGCGGCTGCCTTGTCCTTCTGGGAGGAGCTGG27690.13501086815547558No Hit
TGGAGCCAGACCATAAATACAAGATGAACCTGTACGGCTTCCACGGTGGC27430.13374316047326454No Hit
TGGAGGCGTGCATTTGTTAGGTATAATGCACTGAGGGGCGGGGCCGCTCC26900.1311589871210651No Hit
AGGCCCTCATGAATGACCTCTTTCTTTCCTTTCTTCTTTTCTAGCTACTC26470.12906239364663918No Hit
GCAGGTACCCTCAACTGAGTAAGGGACAATGAACAGCCTCTTGGCCATTC26230.12789220193998282No Hit
GAAATATCCAGTTACTCCTCTAGTCCCATCACCTCGCTTTATGATGTGTA26200.12774592797665077No Hit
GAAGGGTCAGATCCTATCCACCTCCCTGGCCCCAGCTTAGTCCTTTCAGG25730.12545430255111542No Hit
GGAACCCAGTCACATCCAAAGAGTGGTACTGGAACCAGGTGAGGTCGTGG25310.12340646706446683No Hit
TGAAAAGCAAGGCTGTATAGAAGACGGTGACCACGGTGAGGTGAGAGGCG25070.1222362753578105No Hit
GGAGCAGCTTGGTAACTCTGGCTCCCATTTGTTCAGGGCCTGGCACTTCA25020.12199248541892377No Hit
GGTGGCGTGACCTGTAGATGCTGAGGAAGTGTCGGTGACAGGAAGAGGGG24260.11828687834784535No Hit
GAGAAATTATTTAATACTTCAGCCAAGAAGTCTTTCTAGACTAAATTGTT24120.11760426651896247No Hit
CATGCGTCTTCACCTGGAAGGGGTCCATGTGCCCCTCCTTCTGGCCACCA23980.11692165469007959No Hit
TGTGTGAATGCAGTCATCTGTATAAAATGTCATCATTACCTTCATCATTT23870.11638531682452878No Hit
AGAGAACCCTTACAAATTCGAAGAATGTGGTAAAGCCTTTTATTGGTTTT23830.11619028487341938No Hit
GACATTACCACAGGACAATTTGGCTACAGACATGTGGTCACAGTAGGTAT23750.1158002209712006No Hit
GGTGTCATCTGTGGAAGCTGAAGAAAGGCCGGTGACAGGAAGTGGGGTGG23620.11516636713009508No Hit
CTGTCCTAATTCCCCACTCCTCTAGGCAGGGCCAGCATTTCTGCGCGGGG23530.11472754524009895No Hit
GTAGTCGCCATGGAGGTAACTGGAATGATACGCGCCGTGGTGGTCTTGGT23250.11336232158233324No Hit
GGTGGAGGTGAGGCAGATGCCCAGCAGGCGGCACACGTGGGGGTTGTCCA23070.11248467780234096No Hit
CCTGAAGTCTGGCTATGGAAAATATCTTGGTATAAATTCAGATGAACTTG22980.11204585591234484No Hit
GCTCAGACAAATACTTCCATGCTCGGGGGAACTATGATGCTGCCAAAAGG22830.11131448609568463No Hit
GTAATTGCAAAGGTTGGGACTGCCACCTTCCTGAGAGGGGTATTACTCAT22590.11014429438902827No Hit
ATCCTGGCTCAGCAACCTACCCTTTTCTCCCTGGGACTCCTTGTCTGCTG22040.10746260506127416No Hit
TGCCCTCATGATTCTCCTGATTCACCGGGACCACCGGCTCCACACGCCCA21950.10702378317127804No Hit
GGAGGAAGAAGATCTGCACACCACAGCCAGCGCGGGAGATGGCCTTACTT21870.10663371926905925No Hit
CATCTAATTTCCCGGGCCATACCCTGCAGGTACTGGATGATGCTGAGGTT21790.10624365536684047No Hit
GATGTTTGCTGGAATGGATACTTTCCTACTGGCTGTGATAGCCTATGACC21780.10619489737906312No Hit
CGGATGAGAAAGGAGTCGATGAAGTCCCGTGGGGAATTGGGATCCAGCGT21710.10585359146462169No Hit
AAACTGACAAAGTCCATCTGGAGGCGGGGGCCGCCGTAGCGGATGAGCAT21640.10551228555018026No Hit
AAGTGGTGACATCAGAACCTGCCCTCCACACCCAACAGCTGGACTGCAGC21570.10517097963573883No Hit
GTTCTCTTCGGTGATGAAACACCTGCCAGGACCACAGCAACAGGCCTTTA21430.10448836780685596No Hit
GGGATGAGTGGAAGGTTCTCTTACGGAGTTACTCGTCATCTTCCTCAGGG21390.10429333585574656No Hit
AGGAGCTCACTCACTTTAATAGCAGTGCTTTCATCAAAGGACTTTGGTGC21350.10409830390463717No Hit
AACTGGAGGGACATTTGAAGGGGCCTTGAGCAGGAGGGGAGCTGGGTGCA21320.10395202994130513No Hit
GCTGAGTTTGAATGCATCCACCCTGAGAAGCAGCAGAAAAAGAAAAGCTA20950.10214798439354326No Hit
CCGCATTAATATCCCAGAGAGAGCAGGTGGACCAGAAGGCAACTTAAACC20860.10170916250354714No Hit
GATATAAACATGAGCCCTGAGAACCAGAGCAGCGTGTCCGAGTTCCTCCT20790.10136785658910571No Hit
CCACCTCTCCTTTGTAGACATTTGCTATTCTTCCAATGTTACTCCAAATA20670.10078276073577754No Hit
GATCCACCTCCTGCTGGTAGCGCTTACGGTCCCGCATGGCGTTCTCCTTG20550.10019766488244936No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)