FastQCFastQC Report
Wed 8 May 2024
EGAF00001129278

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001129278
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2316621
Total Bases347.4 Mbp
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGTCCTTCCATGGTGACTCTGGCTCTTGCAGGAGGTGGGCTACTGCAG160850.6943302335599997No Hit
AGTGTGCCTTGGCTGAAAGCTGTGATCACCTGGCCAGGGTGGCCATCCCC110690.47780797981197615No Hit
AGCCACTATCAAATGCTGTGAAGAGAAATGTACCCAGATGTATCATTATC98620.4257062333458947No Hit
GCTTTGTTGGCCTCAAATAGCTGCTTTATTAGATGCAATGAAGCAGGGGA89770.3875040414465724No Hit
GCTTTGTTGGCCTCAAATAGCTGCTTTATTAGATGTAATGAAGCAGGGGA89190.38500039497181454No Hit
GGCATACAGTGGACGAGAAACAGTATATTCCAATGCATCCCTGCTGATCC58340.25183230230581527No Hit
GTATTTCATTATGTCCTGAGGAACAGTTGGTTATAGAAAGGCTACAAGGA55340.23888240674672293No Hit
GGTTGATATGGGAGTAAAGACCTTGGCATGGTACATACCCCTCATGCAAG54690.23607659604225292No Hit
AGGCCCTCATGAATGACCTCTTTCTTTCCTTTCTTCTTTTCTAGCTACTC53260.22990381249241892No Hit
CATGCGTCTTCACCTGGAAGGGGTCCATGTGCCCCTCCTTCTGGCCACCA51040.2203208897786906No Hit
AGTAAATACATTGGTTGAAGTTTAAAAGTCTATCCGTAGTAAACTTACAT50980.22006189186750874No Hit
GGAGGAAGAAGATCTGCACACCACAGCCAGCGCGGGAGATGGCCTTATTT50260.2169539169333266No Hit
CTGCTGTCCAGACCATATTGACTTTCCATTTGCCCTACTGTGGACCCAAC49290.21276678403588672No Hit
GGTTTGAAGACCACTTAAAAGCTTTGCCACATTCTTGACATTTGTAGGGT45090.1946369302531575No Hit
CACTTAATTTTGTTTCTGATCTCACCATCCAAGCCATTCAGATCGGAAGA44720.19303977646753612No Hit
CCAACCTCAAGCACAATCAACCTCAAGTTAACCTTTTAGATCGGAAGAGC42860.1850108412208989No Hit
AACAGAGAGAATTTTCACTATGGATCAGTGGTGACCTACCGCTGCAATCC42410.18306835688703504No Hit
ACGTCAGCCCCAGGAAATGCCCGGTTTGGCCAGGGTTCAGGACCCATTGT41720.1800898809084438No Hit
CTCCATCATAGGACCCAAAAGGGGGGAACATGCCTGGACCCACAGACTGC41540.17931288717489827No Hit
TTGCCTCCTTGGTATGAAACATCTTTCACACAACCCTCAATTGTAGGAGT40020.1727516067582915No Hit
GGAAAATGCTGGCTGACCTAAAGCCACCTCCTTACTTTGCCTCCTTCTGC39060.16860764017938196No Hit
GGTGGAGGTGAGGCAGATGCCCAGCAGGCGGCACACGTGGGGGTTGTCCA38870.1677874801273061No Hit
ATACACCCCCATAATGCTCAGAATTTCTTAAATGTAATTTACTGAGATCG37720.1628233534963207No Hit
AAAAGGTTAACTTGAGGTTGATTGTGCTTGAGGTTGGAGATCGGAAGAGC37610.16234852399248734No Hit
CCGCATTAATATCCCAGAGAGAGCAGGTGGACCAGAAGGCAACTTAAACC37540.16204635976277518No Hit
GCCCACAGCATCCTTACCTGCTGTGTGACTCGTTCTGGGTACCATCTCCC36210.15630523939824426No Hit
AGGTGACACCATGCCTCTTCCTGTCACTAGCCCTTCCTCAGCATCCACAG36180.15617574044265334No Hit
GGCAAGTGGAAAGCCTTCTCCACCTGTGGCTTACACCTCACTGTGGTGTC35890.1549239172052744No Hit
CAGTAAATTACATTTAAGAAATTCTGAGCATTATGGGGGTGTATAGATCG35270.15224760545639532No Hit
GCCTGTTTCTCCATCTGAACTGCGCTCCTGGCCGCCCATGGCCTCCCAGT35260.15220443913786502No Hit
CATCATGATCAACCAGTGAAGCCCCTGGATCGAGCAGTCTTCTGGATTGA35260.15220443913786502No Hit
AGGAGTCATGGGAGAAAACAACACCCTGGTCTGGAAGTACGCAGACGCCG34840.1503914537595921No Hit
ACTTTCACCCAAACCTCCTACACCCTGTTCGTCCGCGAGAACAACAGCCC33680.1453841608100764No Hit
GAACACCCCACACATTAGGGCGGCTGCCTTGTCCTTCTGGGAGGAGCTGG33510.14465033339506117No Hit
GCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTGGATCCCAG33290.1437006743873944No Hit
GTAGGTGTCTTCTCCTCGATCCCTGGGATGGGAGCCTTGTGGGGTAGTAT33130.14301001329090945No Hit
GAATGGCTTGGATGGTGAGATCAGAAACAAAATTAAGTGAGATCGGAAGA32830.14171502373500022No Hit
CTCAGTCCATTGCCCCAAGGCTCACTTGTTAGCTTGCCTGGGGAAAGGGG32620.14080853104586377No Hit
GGATACTCATTCTACCCAGTGTCACGAGAAATGAAACAGGACCCTATCAA31820.13735522556343915No Hit
AGTGTGCCTTGGCTGAAAGCTGTGATCACCCGGCCAGGGTGGCCATCCCC31800.13726889292637853No Hit
ATCCTGAAACTGGCCTGTGCAGACACCTCAGCCAACGAGATGGTCATCTT31790.13722572660784824No Hit
CACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTTTCGGAG30970.13368608848836303No Hit
CAGGAACGTACTGGTGAAAACACCGCAGCATGTCAAGATCACAGATTTTG30690.1324774315695144No Hit
AGTAAATACATTGGTTGAAGTTTAAAAGTCTATGCGTAGTAAACTTACAT30400.13122560833213548No Hit
GTGTGATCTGAACCCTTTGCTCAACCTCGCCTGCACTGACACCCATATGC30360.13105294305801424No Hit
GGAACAGACGATGGACACATAGGACAGCACTATCAGGACAAAGCAGCCCG30360.13105294305801424No Hit
GCAGGTACCCTCAACTGAGTAAGGGACAATGAACAGCCTCTTGGCCATTC30330.13092344410242332No Hit
CTGAGGAGCAGTAAGGGATGGCATCAGCAACGCGAGCCACAGGCAGGCCT29710.12824713235354424No Hit
CCGTGTCCAAGGAGGTCATGTACACAGCCTTCAGATCGGAAGAGCACACG29000.12518232373789237No Hit
CCACCTCTCCTTTGTAGACATTTGCTATTCTTCCAATGTTACTCCAAATA28790.12427583104875593No Hit
GCGGGACCACAATCCTTCGCTTTTCTCCAATGCAAACACCAAGTAGACCT28770.12418949841169533No Hit
CAGACCCATGCTGACCATAGCTGGAAGATGAACCTGAGCTAGATCCGTGT28340.12233334671489207No Hit
GTGCTCCTTCATTTTTGATCTCCCCACTAGGTGCAATACCCTGATTATCA28300.12216068144077084No Hit
GGGATGAGTGGAAGGTTCTCTTACGGAGTTACTCGTCATCTTCCTCAGGG28140.12147002034428593No Hit
GGTGGAGGCCATAGGAATGGGAACATCATGGGCCACATCCCCTTGCCCTT27870.12030452974396762No Hit
CCAGGAAGCCTACGTGATGGCCAGCGTGGACAACCCCCACGTGTGCCGCC27820.12008869815131609No Hit
CTGGGTTGGGCATGTGAAATTCCATTTTCTGTTTGAGATCGGAAGAGCAC27520.11879370859540686No Hit
GAAGCTCAATGTCTGCTTTCGGAACTACAACGCCAAGACCGAGAACCTAG27420.11836204541010376No Hit
CATACACCCTCATCCTGCATCTCATCCACAGGATGAATTCTGCCGCCGGC27110.11702388953566423No Hit
GCAGAAGAGAAGGGATCCATCCTAAGCATGACTCCTGAGCAGCTGGGCTC26780.11559940102416406No Hit
CGCCCCTCAGTGCATTATACCTAACAAATGCACGCCTCCAAATGTGGAAA26500.11439074410531545No Hit
CAGGATTCCCTCTACCTACCTTCCCCCCATTTAGGCGTGCATTAGGCCTC26450.11417491251266391No Hit
GTACTGGGAGCCAATATTGTCTTTGTGTTCCCGGACATAGTCCAGGAGGC25990.11218926186026977No Hit
CAAGAGAGGAAATGTTCTGTTCTCCTTCACTTTCCACTCACCCGTAGGTG25970.11210292922320915No Hit
TTACAGTCAGCATGGATCTGGCTCAGGTCACTCCTCTGGCTACGGACAAC25940.11197343026761823No Hit
GACAAGCTGGTGTCTGTATTCTACACCATCCTCACTCCCATGCTGAATCC25910.11184393131202729No Hit
GACCTGCGCTGTGAACTGCCCAAGCTGGAGAAGCGGCTGCGTGCCACGGC25800.11136910180819391No Hit
CAATGTCCACAAAGATGACCATCTCGTTGGCTGAGGTGTCTGCACAGGCC25750.11115327021554237No Hit
CTGCGATTTTTCTGCATCAGGCCCAGCAGCACTGTACAGCAGGGTTCACC25480.10998777961522407No Hit
GAGTCTCAAAGCCATGTTATTCTGCCTTTTTAAACTATCATCCTGTAATC25270.1090812869260876No Hit
GCGGGACCACAATCCTTCGCTTCTCTCCAATGCAAACACCAAGTAGACCT25250.10899495428902699No Hit
AGAATCCAGCTTGTGTTCCAGCTCTTCCCATCAGATCGGAAGAGCACACG25230.10890862165196637No Hit
GTTTAGGGTCATGGGTTCTTGTTCTTTATTGTATCGGAGATCGGAAGAGC25230.10890862165196637No Hit
GGTTAGGTAGGAGCTTACTCAGTGCATCTGGAGGATGGAAAACCAATGGC24810.10709563627369345No Hit
GAAGCAAGGATGTAAAACTCAGTGATCACCAGGGCGATGAAGAGAAGACA24800.10705246995516314No Hit
AGAGGGCTCTTCTATACTTACCTGTCCCATCATTGTCTCCTTATACTGCT24620.10627547622161759No Hit
GAAGTAGATGATGGACAGGATCACAGGTTAAGATCACAGATCGGAAGAGC24600.10618914358455699No Hit
AAGGCACACTTACCACTGTTTTCTTGGCTCCAGTCCAAACCTGAGAGTAG24590.10614597726602668No Hit
GCTTTGTATATGAGGCCTGTGGGGCAAGAACCTTTGTGCAGCGTTTCCGC24440.10549848248807207No Hit
TGTGATCTTAACCTGTGATCCTGTCCATCATCTACTTCAGATCGGAAGAG24280.10480782139158713No Hit
CCTCTACTTCATAGTGCTTATTGGCGGGCTGGTGGGTGTCATTTCCATTC24230.10459198979893561No Hit
GAAGGCTGTGTACATGACCTCCTTGGACACGGAGATCGGAAGAGCACACG24220.1045488234804053No Hit
CAGACCCATGCTGACCATAGCGGGAAGACGAACCTGAGCTAGATCCGTGT24190.10441932452481438No Hit
GAAATATCCAGTTACTCCTCTAGTCCCATCACCTCGCTTTATGATGTGTA24180.10437615820628407No Hit
GAGAGGGCAATTAAGACTTCTTACTCCTCGTCCTGAAGGGTCTCCTCCTC24180.10437615820628407No Hit
GGCGAGTGTACCAGTACCTGGCTGCCGACTGCACCTCCACCTTCCCTCGG24130.10416032661363253No Hit
GGATGACCTCATGCTCATCAAGCTGGCTAAGCCTGCCATGCTCAATCCCA23920.10325383392449607No Hit
GATGGGAAGAGCTGGAACACAAGCTGGATTCTAGATCGGAAGAGCACACG23720.1023905075538899No Hit
GATCCACCTCCTGCTGGTAGCGCTTACGGTCCCGCATGGCGTTCTCCTTG23560.10169984645740499No Hit
CTGCGTTGATGGAGACCAGTGAGACTCTGATATCAGATCGGAAGAGCACA23200.10014585899031392No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)