FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001129279

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001129279
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2316621
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGTCCTTCCATGGTGACTCTGGCTCTTGCAGGAGGTGGGCTACTGCAG159480.6884164479213475No Hit
AGTGTGCCTTGGCTGAAAGCTGTGATCACCTGGCCAGGGTGGCCATCCCC99020.42743288608710706No Hit
AGCCACTATCAAATGCTGTGAAGAGAAATGTACCCAGATGTATCATTATC98300.42432491115292487No Hit
GGTTGATATGGGAGTAAAGACCTTGGCATGGTACATACCCCTCATGCAAG57530.24833583050486033No Hit
GGCATACAGTGGACGAGAAACAGTATATTCCAATGCATCCCTGCTGATCC51350.22165904565313016No Hit
AGGCCCTCATGAATGACCTCTTTCTTTCCTTTCTTCTTTTCTAGCTACTC48930.21121279656879569No Hit
GGTGGAGGTGAGGCAGATGCCCAGCAGGCGGCACACGTGGGGGTTGTCCA48200.20806165531608323No Hit
AGTAAATACATTGGTTGAAGTTTAAAAGTCTATCCGTAGTAAACTTACAT47930.20689616471576489No Hit
CTGCTGTCCAGACCATATTGACTTTCCATTTGCCCTACTGTGGACCCAAC47020.2029680297295069No Hit
GAATGGCTTGGATGGTGAGATCAGAAACAAAATTAAGTGAGATCGGAAGA44530.1922196164154603No Hit
GCTTTGTTGGCCTCAAATAGCTGCTTTATTAGATGTAATGAAGCAGGGGA43710.18867997829597505No Hit
GCTTTGTTGGCCTCAAATAGCTGCTTTATTAGATGCAATGAAGCAGGGGA43230.18660799500652028No Hit
GGTTTGAAGACCACTTAAAAGCTTTGCCACATTCTTGACATTTGTAGGGT42690.18427701380588365No Hit
AAAAGGTTAACTTGAGGTTGATTGTGCTTGAGGTTGGAGATCGGAAGAGC42530.18358635270939871No Hit
TTGCCTCCTTGGTATGAAACATCTTTCACACAACCCTCAATTGTAGGAGT41200.1778452323448678No Hit
CCAACCTCAAGCACAATCAACCTCAAGTTAACCTTTTAGATCGGAAGAGC38820.1675716485346546No Hit
CAGGAACGTACTGGTGAAAACACCGCAGCATGTCAAGATCACAGATTTTG38400.16575866315638163No Hit
CAGTAAATTACATTTAAGAAATTCTGAGCATTATGGGGGTGTATAGATCG38290.16528383365254826No Hit
CATGCGTCTTCACCTGGAAGGGGTCCATGTGCCCCTCCTTCTGGCCACCA38000.16403201041516935No Hit
GCCCACAGCATCCTTACCTGCTGTGTGACTCGTTCTGGGTACCATCTCCC36240.15643473835383517No Hit
ATACACCCCCATAATGCTCAGAATTTCTTAAATGTAATTTACTGAGATCG36030.15552824566469872No Hit
AGGTGACACCATGCCTCTTCCTGTCACTAGCCCTTCCTCAGCATCCACAG36020.15548507934616843No Hit
GGCAAGTGGAAAGCCTTCTCCACCTGTGGCTTACACCTCACTGTGGTGTC35360.1526361023231681No Hit
CACTTAATTTTGTTTCTGATCTCACCATCCAAGCCATTCAGATCGGAAGA35340.15254976968610748No Hit
CCGCATTAATATCCCAGAGAGAGCAGGTGGACCAGAAGGCAACTTAAACC35150.15172960963403165No Hit
GGGATGAGTGGAAGGTTCTCTTACGGAGTTACTCGTCATCTTCCTCAGGG34610.14939862843339502No Hit
ACGTCAGCCCCAGGAAATGCCCGGTTTGGCCAGGGTTCAGGACCCATTGT33600.14503883026183395No Hit
GAACACCCCACACATTAGGGCGGCTGCCTTGTCCTTCTGGGAGGAGCTGG33340.14391650598004593No Hit
GGAACAGACGATGGACACATAGGACAGCACTATCAGGACAAAGCAGCCCG33140.14305317960943978No Hit
CTCCATCATAGGACCCAAAAGGGGGGAACATGCCTGGACCCACAGACTGC32930.14214668692030333No Hit
GCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTGGATCCCAG32310.13947037517142424No Hit
GTAGGTGTCTTCTCCTCGATCCCTGGGATGGGAGCCTTGTGGGGTAGTAT31890.1376573897931513No Hit
GGATACTCATTCTACCCAGTGTCACGAGAAATGAAACAGGACCCTATCAA31810.13731205924490886No Hit
GCGGGACCACAATCCTTCGCTTTTCTCCAATGCAAACACCAAGTAGACCT31660.13666456446695424No Hit
CAAGAGAGGAAATGTTCTGTTCTCCTTCACTTTCCACTCACCCGTAGGTG31350.1353264085925147No Hit
CACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTTTCGGAG31050.13403141903660548No Hit
CGGAGCATGTAGGTGTAGAAGGAAGCACCGAAGAGCAGCAGCACTATGAT30660.13234793261392347No Hit
GGAAAATGCTGGCTGACCTAAAGCCACCTCCTTACTTTGCCTCCTTCTGC30430.1313551072877264No Hit
TGCCTCCTTGGTATGAAACATCTTTCACACAACCCTCAATTGTAGGAGTC29940.1292399576797413No Hit
GGAACCCAGTCACATCCAAAGAGTGGTACTGGAACCAGGTGAGGTCGTGG29740.12837663130913515No Hit
CCAGGAAGCCTACGTGATGGCCAGCGTGGACAACCCCCACGTGTGCCGCC29570.12764280389411992No Hit
GTATTTCATTATGTCCTGAGGAACAGTTGGTTATAGAAAGGCTACAAGGA29340.12664997856792284No Hit
ATCCTGAAACTGGCCTGTGCAGACACCTCAGCCAACGAGATGGTCATCTT29320.12656364593086222No Hit
GAAGGCTGTGTACATGACCTCCTTGGACACGGAGATCGGAAGAGCGTCGT28960.12500965846377116No Hit
GTGTGATCTGAACCCTTTGCTCAACCTCGCCTGCACTGACACCCATATGC28720.12397366681904376No Hit
GAAGCAAGGATGTAAAACTCAGTGATCACCAGGGCGATGAAGAGAAGACA28550.12323983940402854No Hit
GCAGAAGAGAAGGGATCCATCCTAAGCATGACTCCTGAGCAGCTGGGCTC28210.12177218457399808No Hit
AGGAGTCATGGGAGAAAACAACACCCTGGTCTGGAAGTACGCAGACGCCG27940.12060669397367978No Hit
AGTAAATACATTGGTTGAAGTTTAAAAGTCTATGCGTAGTAAACTTACAT27910.12047719501808885No Hit
CAAACAGAAAATGGAATTTCACATGCCCAACCCAGAGATCGGAAGAGCGT27850.12021819710690701No Hit
AGTGTGCCTTGGCTGAAAGCTGTGATCACCCGGCCAGGGTGGCCATCCCC27850.12021819710690701No Hit
GGCGAGTGTACCAGTACCTGGCTGCCGACTGCACCTCCACCTTCCCTCGG27710.11961386864748269No Hit
ACTTTCACCCAAACCTCCTACACCCTGTTCGTCCGCGAGAACAACAGCCC27280.11775771695067946No Hit
GAAATATCCAGTTACTCCTCTAGTCCCATCACCTCGCTTTATGATGTGTA27120.11706705585419452No Hit
GGAGCAGCTTGGTAACTCTGGCTCCCATTTGTTCAGGGCCTGGCACTTCA26900.11611739684652778No Hit
GTGTGAATGCAGTCATCTGTATAAAATGTCATCATTACCTTCATCATTTC26390.11391591460148207No Hit
GCACTGTCAGCAGAAAAACGCTGTGGACCACCACCAAGTTAATGACCGCC26370.11382958196442146No Hit
CTCAGTCCATTGCCCCAAGGCTCACTTGTTAGCTTGCCTGGGGAAAGGGG26370.11382958196442146No Hit
CCGATACAATAAAGAACAAGAACCCATGACCCTAAACAGATCGGAAGAGC26260.11335475246058808No Hit
GAGAGGGCAATTAAGACTTCTTACTCCTCGTCCTGAAGGGTCTCCTCCTC26050.1124482597714516No Hit
GGAGGAAGAAGATCTGCACACCACAGCCAGCGCGGGAGATGGCCTTATTT25990.11218926186026977No Hit
GCGGGACCACAATCCTTCGCTTCTCTCCAATGCAAACACCAAGTAGACCT25920.1118870976305576No Hit
GATGGGAAGAGCTGGAACACAAGCTGGATTCTAGATCGGAAGAGCGTCGT25760.11119643653407268No Hit
GCCAATGAGTTCGGCAACTCCTGGGAGGAGGTGGTGCCCGACTCTCCCTG25740.11111010389701208No Hit
GTGTGAATGCAGTCATCTGTATAAAATGTCATCAATACCTTGATCATTTC25150.10856329110372391No Hit
GAAGCTCAATGTCTGCTTTCGGAACTACAACGCCAAGACCGAGAACCTAG25050.10813162791842085No Hit
GTGATCTTAACCTGTGATCCTGTCCATCATCTACTTCAGATCGGAAGAGC25040.10808846159989054No Hit
GAAGTAGATGATGGACAGGATCACAGGTTAAGATCACAAGATCGGAAGAG25010.10795896264429962No Hit
CCGTGTCCAAGGAGGTCATGTACACAGCCTTCAGATCGGAAGAGCGTCGT25000.10791579632576931No Hit
GCACCACGAAATAGTTGTTCCAGACGTACAGCTGGTTGTCGCGAGGGTTG24750.10683663836251162No Hit
GATCCACCTCCTGCTGGTAGCGCTTACGGTCCCGCATGGCGTTCTCCTTG24750.10683663836251162No Hit
GTACTGGGAGCCAATATTGTCTTTGTGTTCCCGGACATAGTCCAGGAGGC24440.10549848248807207No Hit
GTCAAAGGAGTGTTGGAGTCAACAGGAGTTGTTGAAAGGGTGCTAGCCTC24260.10472148875452653No Hit
TGTGTGAATGCAGTCATCTGTATAAAATGTCATCAATACCTTGATCATTT24250.10467832243599622No Hit
CAATGTCCACAAAGATGACCATCTCGTTGGCTGAGGTGTCTGCACAGGCC24180.10437615820628407No Hit
AGAATCCAGCTTGTGTTCCAGCTCTTCCCATCAGATCGGAAGAGCGTCGT24170.10433299188775376No Hit
CCACCTCTCCTTTGTAGACATTTGCTATTCTTCCAATGTTACTCCAAATA24150.10424665925069315No Hit
AAAGTACTCACTCTCCACACTTGCCCTTTCCTAACACTTTCCTGAGTGCT24020.10368549710979913No Hit
GGATGACCTCATGCTCATCAAGCTGGCTAAGCCTGCCATGCTCAATCCCA23820.10282217073919299No Hit
GTCGACAGACAGGATTATCGTCTCCATCTTTCTCCAAAATAAGATTGGTC23770.10260633914654146No Hit
GAGTCTCAAAGCCATGTTATTCTGCCTTTTTAAACTATCATCCTGTAATC23750.10252000650948084No Hit
AGAGGGCTCTTCTATACTTACCTGTCCCATCATTGTCTCCTTATACTGCT23540.10161351382034438No Hit
AAGGCACACTTACCACTGTTTTCTTGGCTCCAGTCCAAACCTGAGAGTAG23530.10157034750181407No Hit
GATATCAGAGTCTCACTGGTCTCCATCAACGCAGAGATCGGAAGAGCGTC23520.10152718118328376No Hit
GACAAGCTGGTGTCTGTATTCTACACCATCCTCACTCCCATGCTGAATCC23370.10087968640532913No Hit
GACCTGCGCTGTGAACTGCCCAAGCTGGAGAAGCGGCTGCGTGCCACGGC23360.10083652008679883No Hit
GTCCGGCGATAATAAGCAAACTAGAAGTATAAGGTGACAAGATCGGAAGA23280.10049118953855637No Hit
TGGAGGCGTGCATTTGTTAGGTATAATGCACTGAGGGGCGGGGCCGCTCC23250.10036169058296544No Hit
GGCCAATTGTGAGGACATTCTCATGAACTTCCTGGTGTCTGCTGTGACAA23220.10023219162737453No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)