FastQCFastQC Report
Wed 8 May 2024
EGAF00001129283

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001129283
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2306253
Total Bases345.9 Mbp
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCACTATCAAATGCTGTGAAGAGAAATGTACCCAGATGTATCATTATC132750.5756090073378767No Hit
GATGTCCTTCCATGGTGACTCTGGCTCTTGCAGGAGGTGGGCTACTGCAG128310.5563569998608132No Hit
AGTGTGCCTTGGCTGAAAGCTGTGATCACCTGGCCAGGGTGGCCATCCCC85850.3722488382670939No Hit
GGCATACAGTGGACGAGAAACAGTATATTCCAATGCATCCCTGCTGATCC73500.31869877242436107No Hit
GCTTTGTTGGCCTCAAATAGCTGCTTTATTAGATGCAATGAAGCAGGGGA63280.2743844669253547No Hit
AGTAAATACATTGGTTGAAGTTTAAAAGTCTATGCGTAGTAAACTTACAT58190.2523140349302527No Hit
CAGACCCATGCTGACCATAGCTGGAAGATGAACCTGAGCTAGATCCGTGT54500.23631405574323375No Hit
GCTTTGTTGGCCTCAAATAGCTGCTTTATTAGATGTAATGAAGCAGGGGA51280.22235201428464266No Hit
GGTTTGAAGACCACTTAAAAGCTTTGCCACATTCTTGACATTTGTAGGGT45990.1994143747455288No Hit
AGTAAATACATTGGTTGAAGTTTAAAAGTCTATCCGTAGTAAACTTACAT45940.19919757285952583No Hit
TTGCCTCCTTGGTATGAAACATCTTTCACACAACCCTCAATTGTAGGAGT43770.18978837100699705No Hit
TTACAGTCAGCATGGATCTGGCTCAGGTCACTCCTCCAGCTACGAACAAC42530.1844116842341235No Hit
CTGCTGTCCAGACCATATTGACTTTCCATTTGCCCTACTGTGGACCCAAC41500.17994556538246237No Hit
TGTGTGAATGCAGTCATCTGTATAAAATGTCATCATTACCTTCATCATTT38620.1674577767486915No Hit
GCCCACAGCATCCTTACCTGCTGTGTGACTCGTTCTGGGTACCATCTCCC38310.1661136050554731No Hit
CAGACCCATGCTGACCATAGCGGGAAGACGAACCTGAGCTAGATCCGTGT37300.16173420695821317No Hit
AGGTGACACCATGCCTCTTCCTGTCACTAGCCCTTCCTCAGCATCCACAG37020.16052011639659655No Hit
TGTGTGAATGCAGTCATCTGTATAAAATGTCATCAATACCTTGATCATTT37010.16047675601939596No Hit
GAAATATCCAGTTACTCCTCTAGTCCCATCACCTCGCTTTATGATGTGTA36910.16004315224739002No Hit
ACTTTCACCCAAACCTCCTACACCCTGTTCGTCCGCGAGAACAACAGCCC36410.15787513338736037No Hit
GATCCACCTCCTGCTGGTAGCGCTTACGGTCCCGCATGGCGTTCTCCTTG36160.1567911239573455No Hit
TTACAGTCAGCATGGATCTGGCTCAGGTCACTCCTCTGGCTACGGACAAC35980.15601063716773486No Hit
GACCTGCGCTGTGAACTGCCCAAGCTGGAGAAGCGGCTGCGTGCCACGGC35450.1537125371761034No Hit
GTAGGTGTCTTCTCCTCGATCCCTGGGATGGGAGCCTTGTGGGGTAGTAT35260.15288869000929214No Hit
GGATACTCATTCTACCCAGTGTCACGAGAAATGAAACAGGACCCTATCAA35080.15210820321968144No Hit
GTGTGAATGCAGTCATCTGTATAAAATGTCATCATTACCTTCATCATTTC33060.1433494070251616No Hit
GTGTGAATGCAGTCATCTGTATAAAATGTCATCAATACCTTGATCATTTC32990.14304588438475743No Hit
CATCATGATCAACCAGTGAAGCCCCTGGATCGAGCAGTCTTCTGGATTGA32490.14087786552472778No Hit
GAACACCCCACACATTAGGGCGGCTGCCTTGTCCTTCTGGGAGGAGCTGG32200.13962041458591057No Hit
GTATTTCATTATGTCCTGAGGAACAGTTGGTTATAGAAAGGCTACAAGGA32060.13901336930510225No Hit
TACAGTCAGCATGGATCTGGCTCAGGTCACTCCTCCAGCTACGAACAACA31270.13558789950625538No Hit
GTCAAAGGAGTGTTGGAGTCAACAGGAGTTGTTGAAAGGGTGCTAGCCTC31140.13502421460264766No Hit
GGTTGATATGGGAGTAAAGACCTTGGCATGGTACATACCCCTCATGCAAG31110.1348941334710459No Hit
GGGATGAGTGGAAGGTTCTCTTACGGAGTTACTCGTCATCTTCCTCAGGG30530.1323792315934115No Hit
AAAAGGTTAACTTGAGGTTGATTGTGCTTGAGGTTGGAGATCGGAAGAGC30040.13025457311058242No Hit
GGAACCCAGTCACATCCAAAGAGTGGTACTGGAACCAGGTGAGGTCGTGG29670.12865023915416043No Hit
TGCCTCCTTGGTATGAAACATCTTTCACACAACCCTCAATTGTAGGAGTC28920.12539821086411596No Hit
GAATGGCTTGGATGGTGAGATCAGAAACAAAATTAAGTGAGATCGGAAGA28470.12344699389008926No Hit
CTCAGTCCATTGCCCCAAGGCTCACTTGTTAGCTTGCCTGGGGAAAGGGG28130.12197274106526908No Hit
CCAACCTCAAGCACAATCAACCTCAAGTTAACCTTTTAGATCGGAAGAGC28110.1218860203108679No Hit
ACGTCAGCCCCAGGAAATGCCCGGTTTGGCCAGGGTTCAGGACCCATTGT27930.1211055335212572No Hit
GGATGACCTCATGCTCATCAAGCTGGCTAAGCCTGCCATGCTCAATCCCA27240.11811366749441626No Hit
CTCCATCATAGGACCCAAAAGGGGGGAACATGCCTGGACCCACAGACTGC27190.11789686560841331No Hit
CAATGTCCACAAAGATGACCATCTCGTTGGCTGAGGTGTCTGCACAGGCC26780.11611909014318897No Hit
CCTGCATATAGTGGACGAGAAACAGCATATTCCAATGCATCCCTGCTGAT26520.11499172033597353No Hit
GCCATCATTTCCTGGGAGAGATGGATGGTGGTCTGCAAGCCCTTTGGCAA26370.11434131467796464No Hit
GCAGGTACCCTCAACTGAGTAAGGGACAATGAACAGCCTCTTGGCCATTC25880.11221665619513556No Hit
AAGGCACACTTACCACTGTTTTCTTGGCTCCAGTCCAAACCTGAGAGTAG25870.11217329581793498No Hit
TGGAGGCGTGCATTTGTTAGGTATAATGCACTGAGGGGCGGGGCCGCTCC25120.10892126752789048No Hit
GGAGGAAGAAGATCTGCACACCACAGCCAGCGCGGGAGATGGCCTTATTT25020.10848766375588453No Hit
GAAGTGAGCTGCAGTCTCTGCTGATTGCCACGACCTGCCTTCGGGAGCAC25000.10840094300148335No Hit
CAGTAAATTACATTTAAGAAATTCTGAGCATTATGGGGGTGTATAGATCG24910.10801069960667803No Hit
ATACACCCCCATAATGCTCAGAATTTCTTAAATGTAATTTACTGAGATCG24860.10779389772067505No Hit
AGGCCCTCATGAATGACCTCTTTCTTTCCTTTCTTCTTTTCTAGCTACTC24800.10753373545747148No Hit
CCAGGTCTATATGAGAAACCTTCTCTCTCAGCCCAGCCGGGCCCCACGGT24740.10727357319426792No Hit
TACAGTCAGCATGGATCTGGCTCAGGTCACTCCTCTGGCTACGGACAACA24720.10718685243986674No Hit
GGTGGAGGTGAGGCAGATGCCCAGCAGGCGGCACACGTGGGGGTTGTCCA24600.10666652791345961No Hit
TGGAGCCAGACCATAAATACAAGATGAACCTGTACGGCTTCCACGGTGGC24600.10666652791345961No Hit
GCCAATGAGTTCGGCAACTCCTGGGAGGAGGTGGTGCCCGACTCTCCCTG24390.10575595999224717No Hit
TGAAAAGCAAGGCTGTATAGAAGACGGTGACCACGGTGAGGTGAGAGGCG24240.10510555433423825No Hit
CCTGAAGTCTGGCTATGGAAAATATCTTGGTATAAATTCAGATGAACTTG23860.10345786000061571No Hit
GTGTGATCTGAACCCTTTGCTCAACCTCGCCTGCACTGACACCCATATGC23820.10328441849181336No Hit
GGCAGTTCCACCATCAAGTACACCACCACCTCCTCCTCCAGCAGGAAGAG23560.10215704868459792No Hit
GTCACATGTGATCCAGGTCTCCTCCGAGGGGCTGGCCAGTCTCTTCTCCC23550.10211368830739732No Hit
CAGACACACGGAGCATGTGTTTGCCCTGCGGCCGGTGGGATTCCGGGACA23540.10207032793019674No Hit
GAAGGGTCAGATCCTATCCACCTCCCTGGCCCCAGCTTAGTCCTTTCAGG23460.101723444912592No Hit
AGTGTGCCTTGGCTGAAAGCTGTGATCACCCGGCCAGGGTGGCCATCCCC23160.10042263359657418No Hit
CATGCGTCTTCACCTGGAAGGGGTCCATGTGCCCCTCCTTCTGGCCACCA23070.10003239020176884No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)