FastQCFastQC Report
Wed 8 May 2024
EGAF00001129329

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001129329
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2875900
Total Bases431.3 Mbp
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATCACTGCCAAGTTAGACAGCCCCAGGGTGACAGTGGCCCAGCTGGAC150800.5243575924058556No Hit
TGGTGATGGGTGTCGATGTTGGGGTCTGTGTGCCGGTGGGTGTTGGGGTT69670.24225459856045065No Hit
AAGCATTCAAGTAGCAGGTATTTCCCATATTCTGGAGCCCAGCCCCCACC67570.2349525365972391No Hit
CTGTGATGATTTGGCTCCTGTGGCAAGACAGCTTGCTCCCAGGGAGAAGC66070.22973677805208803No Hit
GGGAGCGTCACGTCTACCCGCCAGTTCCAGTCATAGCTGACTTGCAGTCC63300.22010501060537568No Hit
GAGAACTCCTTTGTCCATTTGCACGTATGGATCTTTCCTCACTGCTTGAT59660.20744810320247575No Hit
GCCGTAAAACCTCCAGTGACCCGTTTGCCTTCCCTCCTTCCCCCAGGTGA59290.20616154942800516No Hit
GGTAGAGCTGCAAGGACACCGCCTGCACAGGGTGCCCGGGGCTGGGCATT59240.20598769080983342No Hit
CAGCACTACCTGTGGGATCGGCTTCCAGGTGCGGCAGCGCTCCTGCAGCA55350.19246149031607496No Hit
GTGAAGACCAAGAACATGAAGATGATGGTGAAGATGAAGATGATGAAGAT55080.19152265377794778No Hit
GTCTAAGATAATATCGCTTCTGATTCTCTTCTTCTCCATCTTCTTCATCT51980.18074341945130218No Hit
GCACCAAGCCAAGAAAGTCCAGCAGGAGCACGAGCATCCCGATGATCACG49650.17264160784450083No Hit
GACTCTTATCCATCCGTCCTCTCCCAGCTCACCAATCACTCGGCCTAGGC49110.17076393476824644No Hit
GGTGGAGGTGAGGCAGATGCCCAGCAGGCGGCACACGTGGGGGTTGTCCA48060.1671129037866407No Hit
GGAATGAGAGTAAGCGTGAGGCTGGCGGTGCTGGCAGCAGGGTGGGCACG47180.16405299210681873No Hit
GCCCCACCAAGCCAGATAGGAAGGCCACTGTTGGGAGGCAGGAGGGAAAG46860.16294029695051984No Hit
GGTGAAGCAGTGTCCTTGTCCCAGGGGCTGCTGCTGTTGCTGCTGCTGAG46360.1612017107688028No Hit
GGAGGATTCTGTGGCGTATGAAGACCACGGGATAACCGTATTGCCCAAGA46310.16102785215063112No Hit
CTGGTATCTTGTCCTGTTCCTCCTTTTCCCTTTCCAGAGGCCAAGAAGAT45100.1568204735908759No Hit
GTGTCCAACAGGAGATCGACGACGTGATAGGGCAGGTGCGGCGACCAGAG43120.14993567231127647No Hit
CGATGACAGCGCCTTCATGGAGCCGAGGTACCACGTCCGTCGAGAAGATC42400.14743210820960395No Hit
GCATGCACCCTCCTAACTGAGGTCTGGTGGGAGCCATGGAAGGACCAGGG41640.14478945721339406No Hit
CATGCGTCTTCACCTGGAAGGGGTCCATGTGCCCCTCCTTCTGGCCACCA40530.14092979588998228No Hit
AAGGTGCTGACTTCCTTGGGAGATGCCATAAAGCACCTGGATGATCTCAA40510.1408602524427136No Hit
CGATATCCCACTCTCATGAGCTGGCAGCTGTGCCTGAGGATGACCATGTC39110.13599221113390592No Hit
CTCGCAGAAGAAGTGATCGATCTCGTGTGCACCGCAATATGGGAAGCTCA38100.13248026704683752No Hit
GGTCTCTTGCAGTCTGGAAGAGGACGGGCTCGTGGACAGAGGGCGTGGCC38040.13227163670503148No Hit
GAGATTGGAGAAATAATATGCCATGGTATTACATAAGCTTTGGCTTCTCT37910.13181960429778505No Hit
GGATCACGGGTGTCTACACCGCCCTGGGCTATGTGCAGGCTTTCCTGGGA37850.13161097395597898No Hit
AGGGCCTGTCAGTCTTATTTTGGGTGTGTTCAGTGCATTAGTGGACCTCT36590.12722973677805208No Hit
GGAACCCCAGAAAAGACCTTGCCCCTTCTGTCCCTGCAGATGACGTCCAC36570.1271601933307834No Hit
GATATGCTTTATGGTCTGCTCAGTCACCTCGTTCTTAATCCCTTTCTCAA36510.12695156298897736No Hit
CTGTGGATGCTGAGGAAGTGTCGGTGACAGGAAGAGGGGTGGTGTCACCT34910.12138808720748287No Hit
GGAAGACTCGGAGCAGGTAGGCACGAAGCACACAGGCTTGTTACAGACGG34870.12124900031294551No Hit
CTTGGTCTCCTTCACATGTGTGGGCTATGCGGTCACCAAGGCCCACCCTG34670.1205535658402587No Hit
GGGATGTCATATGGGTCACACCCAGGGGGACGATGTCCCCAAAGCGCTGC34130.11867589276400431No Hit
CAACTTCTACCACCAACATCACCCCCAACCACACCAGTACAGGCACCAGA33660.11704162175319031No Hit
CGCAGTGTACCATGCTGTACTCACGCCAGGGGACCACTGAGACCATCGAG33550.11665913279321255No Hit
AAGCTACATTGGTTGCCCATAAATTCTTGATTGTACCAATCTTCCACAAA33520.11655481762230953No Hit
CTTGTCTCCCCAGATGTCAAGAATATGAACACGTGGCTGCTGTTCCTCCC33490.11645050245140652No Hit
GGTAGAGCTGCAAGGACACCGCCTGCACAGGATGCCCGGGGCTGGGCATT33330.11589415487325706No Hit
CCACTACGGTGACACCAACCCCAACACCCACCGGCACACAGACCCCAACA33240.11558120936054801No Hit
GGTAGAGCTGCAAGGACACCACCTGCACAGGGTGCCCGGGGCTGGGCATT33020.1148162314405925No Hit
CAGGAACGTACTGGTGAAAACACCGCAGCATGTCAAGATCACAGATTTTG32990.11471191626968949No Hit
CCTGATTCCTGTCCTCAGCTACCTGGCCAATGACATGGAGGAGGACGACG32710.11373830800792796No Hit
CACAGATGGTGGTGTTGACGGTGATGCACACGGGGCAGCCCTCCTTCTCC32230.1120692652734796No Hit
CTCTGCTCAACCTGTCTTGGCCCGAGGTGGATGAGGGTGCTGTCCTGGGC32190.11193017837894226No Hit
CGGAGCATGTAGGTGTAGAAGGAAGCACCGAAGAGCAGCAGCACTATGAT32090.11158246114259884No Hit
ACCTGTCTGTCCTTTGTGTCCAGGAATTCCCCAGACAGTGGGGAGGGAGG32070.11151291769533014No Hit
GGCGGCAGAATTCATCCTGTGGATGAGATGCAGGATGAGGGTGTATGAGC31880.11085225494627769No Hit
CCTCTCCAGATCCCATGTGAATGACAGCCAGAATAACTCGAGCATAGGAA31830.11067839632810599No Hit
CTCCCACATCGTACCCAGTGGCCTCGTACTCCTTGGCCTCCCTGGGCTCA31710.1102611356444939No Hit
CCATGGCTATCCCCATCTGGGTCTGGCAGAAGTTCTCCTCCAGTTGTGCA31330.10893981014638895No Hit
CTGTGGATGCTGAGGAAGTGTCGGTGACAGGAAGAGGGGTGGCGTGACCT30810.10713168051740324No Hit
GGTAGCCTCATGACAGGAGGACAGGGGAGGTTGGCAGACATACGGATGAG30090.10462811641573073No Hit
GCACTGCTGCTGTTGATAAGACATCCTGCTGGAGTCTCAGGATCTGAAAG30070.10455857296846204No Hit
CAGAGCCTGGAAATGGTTGAGACTACCCTGCCACGATTTTGCTCCCGCTC30030.10441948607392468No Hit
GGTAGAGCTGCAAGGACACCACCTGCACAGGATGCCTGGGGCTGGCCATT28920.1005598247505129No Hit
CAGGACCCTAATAGGAGTATTCATACCAGCAGCAGCAGCAGCAGCAGCAG28820.10021210751416948No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)