FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001129334

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001129334
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2839824
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATCACTGCCAAGTTAGACAGCCCCAGGGTGACAGTGGCCCAGCTGGAC106640.37551622917476574No Hit
TGGTGATGGGTGTCGATGTTGGGGTCTGTGTGCCGGTGGGTGTTGGGGTT60750.21392170782414685No Hit
CTGTGATGATTTGGCTCCTGTGGCAAGACAGCTTGCTCCCAGGGAGAAGC59250.20863969034700744No Hit
AAGCATTCAAGTAGCAGGTATTTCCCATATTCTGGAGCCCAGCCCCCACC54920.1933922665629983No Hit
CCATGGCTATCCCCATCTGGGTCTGGCAGAAGTTCTCCTCCAGTTGTGCA52710.18561009414667953No Hit
CGATGACAGCGCCTTCATGGAGCCGAGGTACCACGTCCGTCGAGAAGATC51270.18053935736862567No Hit
GGAGGATTCTGTGGCGTATGAAGACCACGGGATAACCGTATTGCCCAAGA47220.1662779101803492No Hit
GCCGTAAAACCTCCAGTGACCCGTTTGCCTTCCCTCCTTCCCCCAGGTGA47120.16592577568187325No Hit
GGTCTCTTGCAGTCTGGAAGAGGACGGGCTCGTGGACAGAGGGCGTGGCC44840.1578971091166213No Hit
GAGATTGGAGAAATAATATGCCATGGTATTACATAAGCTTTGGCTTCTCT41880.14747392796173284No Hit
CGATATCCCACTCTCATGAGCTGGCAGCTGTGCCTGAGGATGACCATGTC40910.14405822332651602No Hit
GGACGTTGTTGCTGGACTCAGCCTTCTCGGTGGCAGTTCGGGTTCGGAAG40630.1430722467307833No Hit
CTCGCAGAAGAAGTGATCGATCTCGTGTGCACCGCAATATGGGAAGCTCA40160.14141721458794632No Hit
CTTGTCTCCCCAGATGTCAAGAATATGAACACGTGGCTGCTGTTCCTCCC39780.14007910349373764No Hit
CCTGATTCCTGTCCTCAGCTACCTGGCCAATGACATGGAGGAGGACGACG39630.1395509017460237No Hit
GCACCAAGCCAAGAAAGTCCAGCAGGAGCACGAGCATCCCGATGATCACG38760.13648733160928284No Hit
GGGAGCGTCACGTCTACCCGCCAGTTCCAGTCATAGCTGACTTGCAGTCC38340.1350083667156838No Hit
CTCTTTTGCTTGTCCTTCTTGTTCATGTCAGTGTCCCTGAGCATGACGAT38190.13448016496796986No Hit
GCCCCACCAAGCCAGATAGGAAGGCCACTGTTGGGAGGCAGGAGGGAAAG37490.13201522347863812No Hit
ACCTGTCTGTCCTTTGTGTCCAGGAATTCCCCAGACAGTGGGGAGGGAGG37070.1305362585850391No Hit
GATATGCTTTATGGTCTGCTCAGTCACCTCGTTCTTAATCCCTTTCTCAA36080.12705012705012705No Hit
GAGTCGCACAGCTCAGCCTGTCCCTGACATGTCTCTGTGCTTTGTAGGCG35440.1247964662598809No Hit
GGCGGCAGAATTCATCCTGTGGATGAGATGCAGGATGAGGGTGTATGAGC34710.12222588442100638No Hit
CTGTGGATGCTGAGGAAGTGTCGGTGACAGGAAGAGGGGTGGTGTCACCT34620.12190896337237801No Hit
GGTGGAGGTGAGGCAGATGCCCAGCAGGCGGCACACGTGGGGGTTGTCCA34620.12190896337237801No Hit
CTCTGACGTCTCCCTCTATAAGATGCTCACGTACCTGTGCTGCATCCTCA34620.12190896337237801No Hit
AAGGTGCTGACTTCCTTGGGAGATGCCATAAAGCACCTGGATGATCTCAA34320.12085255987695012No Hit
GGTGAAGCAGTGTCCTTGTCCCAGGGGCTGCTGCTGTTGCTGCTGCTGAG34270.12067649262771213No Hit
GCATGCACCCTCCTAACTGAGGTCTGGTGGGAGCCATGGAAGGACCAGGG34000.11972572948182704No Hit
CCTGATTCCTGTCCTCAGCTATCTGGCCAATGACATGGAGGAGGACGACG33890.11933838153350348No Hit
CAGAGCCTGGAAATGGTTGAGACTACCCTGCCACGATTTTGCTCCCGCTC33690.11863411253655155No Hit
CATGCGTCTTCACCTGGAAGGGGTCCATGTGCCCCTCCTTCTGGCCACCA33400.11761292249097127No Hit
TCCTTGGGTCCATCGTGTAGCACCAGGGCCCATGGCTATCCCCATCTGGG33350.11743685524173329No Hit
CTGGTATCTTGTCCTGTTCCTCCTTTTCCCTTTCCAGAGGCCAAGAAGAT32960.11606353069767703No Hit
CACACTCGTGCAAGGCTTAGAAGGCCAGAAGATCACACGCGTGGCTTGTG32850.11567618274935347No Hit
AGAGCCCACACGATGAAGACCCCCAGGCAGTGACGTATGCCGAGGTGAAA32550.11461977925392558No Hit
GCTGAAATTGAACTCTGCTGTTGCTGTTGCTGTTGCTGTTGCTGCTGCTG32360.11395072370682126No Hit
CCACTACGGTGACACCAACCCCAACACCCACCGGCACACAGACCCCAACA32170.11328166815971694No Hit
CCCCACGATGTCAGAAGGTGCCACAGTCTCATGGAGCATATAATACTGCA31250.11004203077373809No Hit
CCTCTCCAGATCCCATGTGAATGACAGCCAGAATAACTCGAGCATAGGAA31140.10965468282541453No Hit
GCGTTAGTTCGGCCCAATGGCGGCACCGCTGCTTCACACGCGTTTGCCGG31030.10926733487709096No Hit
CTTGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGGCAGCTCGTGG30970.1090560541780054No Hit
CCACCCCTAACATGCTATTGACTTGCCTTCTCCCTTCCCATCTCCACTCC30830.10856306588013906No Hit
AGGCTACTGAGGTGGTCAACCCTCTCCTGGAGCCTGTGGAGCACCGAGAT30820.10852785243029145No Hit
CTGGAGGACCTGCTACGAAATAACAGAAGACCCAAGAAATGGGTGAGTGG30680.10803486413242512No Hit
GTGAAGACCAAGAACATGAAGATGATGGTGAAGATGAAGATGATGAAGAT30440.1071897413360828No Hit
TGTATCATCAAACCCCACTACCCTGCCCAGGTCCCCGTGTCAGTGACATG30210.1063798319895881No Hit
GGTAGCCTCATGACAGGAGGACAGGGGAGGTTGGCAGACATACGGATGAG30170.10623897819019769No Hit
GCACTGCTGCTGTTGATAAGACATCCTGCTGGAGTCTCAGGATCTGAAAG30110.10602769749111213No Hit
ACCTGTCTGTCCTTTGTGTCCAGGAATTCCCCAGACAGTGGGGAAGGAGG30090.10595727059141694No Hit
CACAGATGGTGGTGTTGACGGTGATGCACACGGGGCAGCCCTCCTTCTCC29820.10500650744553185No Hit
GCCGTTGTTCGAGCAGTACATCAACAACCTCAGGAGGCAGCTGGACAGCA29770.10483044019629385No Hit
GAGAACTCCTTTGTCCATTTGCACGTATGGATCTTTCCTCACTGCTTGAT29440.10366839635132317No Hit
GTGCTCTTCAATGACTCAACACGCCTCATCCTCTACAATGATGGTGACAG29310.10321062150330444No Hit
GTTCTCTGTTGCATGCTTCTCCTTCTCCACCTTGGCCAGTGTCAGCTCCA28770.10130909521153424No Hit
GGTAGAGCTGCAAGGACACCGCCTGCACAGGATGCCCGGGGCTGGGCATT28760.10127388176168664No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)