FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001129337

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001129337
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3148636
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATCACTGCCAAGTTAGACAGCCCCAGGGTGACAGTGGCCCAGCTGGAC124880.3966161855482818No Hit
GGGAGCGTCACGTCTACCCGCCAGTTCCAGTCATAGCTGACTTGCAGTCC66610.21155192280085727No Hit
TGGTGATGGGTGTCGATGTTGGGGTCTGTGTGCCGGTGGGTGTTGGGGTT63720.20237334515644237No Hit
GCCGTAAAACCTCCAGTGACCCGTTTGCCTTCCCTCCTTCCCCCAGGTGA62550.19865745040074495No Hit
GGTCTCTTGCAGTCTGGAAGAGGACGGGCTCGTGGACAGAGGGCGTGGCC55990.1778230319414502No Hit
CAGAGCCTGGAAATGGTTGAGACTACCCTGCCACGATTTTGCTCCCGCTC54450.1729320251689938No Hit
CTCGCAGAAGAAGTGATCGATCTCGTGTGCACCGCAATATGGGAAGCTCA50160.1593070777314367No Hit
GGAGGATTCTGTGGCGTATGAAGACCACGGGATAACCGTATTGCCCAAGA49750.1580049265777308No Hit
CCATGGCTATCCCCATCTGGGTCTGGCAGAAGTTCTCCTCCAGTTGTGCA49020.15568646232844952No Hit
GAGATTGGAGAAATAATATGCCATGGTATTACATAAGCTTTGGCTTCTCT47860.15200232735698888No Hit
CGATATCCCACTCTCATGAGCTGGCAGCTGTGCCTGAGGATGACCATGTC47030.14936626526534028No Hit
GGGCTGGACATCTTGAAGTGCCGCACCACAAAGTCCTTGCCGCCCATCAT45560.14469757698254102No Hit
GGCGGCAGAATTCATCCTGTGGATGAGATGCAGGATGAGGGTGTATGAGC44600.14164863769581496No Hit
CCTCTCCAGATCCCATGTGAATGACAGCCAGAATAACTCGAGCATAGGAA41800.13275589810953062No Hit
AAGGTGCTGACTTCCTTGGGAGATGCCATAAAGCACCTGGATGATCTCAA41660.13231126113021638No Hit
CGCCATGTTCTCAGATAAGAAGTACCTGACCGCCAGCCAGCTTGTTCCCC41390.13145374695582468No Hit
TCCTTGGGTCCATCGTGTAGCACCAGGGCCCATGGCTATCCCCATCTGGG40590.1289129642168863No Hit
GGTGAAGCAGTGTCCTTGTCCCAGGGGCTGCTGCTGTTGCTGCTGCTGAG39950.1268803380257356No Hit
CACAGATGGTGGTGTTGACGGTGATGCACACGGGGCAGCCCTCCTTCTCC38830.12332324219122184No Hit
GGTAGCCTCATGACAGGAGGACAGGGGAGGTTGGCAGACATACGGATGAG37800.12005198441483868No Hit
CGATGACAGCGCCTTCATGGAGCCGAGGTACCACGTCCGTCGAGAAGATC37710.1197661463567081No Hit
GCACTGCTGCTGTTGATAAGACATCCTGCTGGAGTCTCAGGATCTGAAAG37510.11913095067197352No Hit
GCATGCACCCTCCTAACTGAGGTCTGGTGGGAGCCATGGAAGGACCAGGG36890.11716184404929628No Hit
CCTGATTCCTGTCCTCAGCTACCTGGCCAATGACATGGAGGAGGACGACG36830.1169712853438759No Hit
AGGCTACTGAGGTGGTCAACCCTCTCCTGGAGCCTGTGGAGCACCGAGAT36650.11639960922761475No Hit
CACTTCTTCTGTGACTTCCCTTCCCTACTAATCCTCTCATGCAATGACAC36080.11458930152612117No Hit
GATATGCTTTATGGTCTGCTCAGTCACCTCGTTCTTAATCCCTTTCTCAA35820.11376354713596618No Hit
CACAACCCCATGCTACCAGCAGTCTAGCTGCCAGCCGGATTGCTGCACCT35800.11370002756749271No Hit
CACTGGAGGAAGACAAGGATCCCACCACGAGCAGGCACGAGACAGCTCCA35480.11268371447191736No Hit
CAACTTCTACCACCAACATCACCCCCAACCACACCAGTACAGGCACCAGA35390.11239787641378679No Hit
AGGGTCACAGATCTTTAACAAGAATGGGGGCACTCAGGGTCTGAGGCCAC35200.11179444051328893No Hit
AAGCATTCAAGTAGCAGGTATTTCCCATATTCTGGAGCCCAGCCCCCACC34690.1101746915172157No Hit
GCACCAAGCCAAGAAAGTCCAGCAGGAGCACGAGCATCCCGATGATCACG34450.10941245669553419No Hit
GGAAGACTCGGAGCAGGTAGGCACGAAGCACACAGGCTTGTTACAGACGG34410.10928541755858727No Hit
GGGATGTCATATGGGTCACACCCAGGGGGACGATGTCCCCAAAGCGCTGC33850.1075068696413304No Hit
GCCCCACCAAGCCAGATAGGAAGGCCACTGTTGGGAGGCAGGAGGGAAAG33690.10699871309354274No Hit
GGCTCACTCACCAGAGATGCGGCCTGCCACGTGCACTCCCATGGTGACTC33640.10683991417235908No Hit
GAGAACTCCTTTGTCCATTTGCACGTATGGATCTTTCCTCACTGCTTGAT33620.10677639460388562No Hit
GTGTCCAACAGGAGATCGACGACGTGATAGGGCAGGTGCGGCGACCAGAG33200.10544248366594297No Hit
CACACTCGTGCAAGGCTTAGAAGGCCAGAAGATCATGTGTGTGGCTTGCG33150.10528368474475931No Hit
GAACCAGCAGATGCCTTCTGTTTTGCCTTCCCAGAACAAGCCTTCTCTCC33100.10512488582357567No Hit
GCACTGCTGCTGTTGATAAGACATCCTGCTGGAGTCTCAGAATCTGAAAG32920.10455320970731453No Hit
GAGGATGAGGAGGAGATGAACGCGGAGCTCACTGCCAAGAAGCGCAAGCT32750.10401329337529013No Hit
CTGTGATGATTTGGCTCCTGTGGCAAGACAGCTTGCTCCCAGGGAGAAGC32230.10236178459498019No Hit
CGGGCAAACTCTCCTCTACTTGGAAGCCCTGACCTAGAGCAGCCCCTCGG31980.10156778998906192No Hit
GCACAGGCTATGAGAAGTACAACTCCATGCGAGCTGACCCCGCGCTGTGC31910.10134547149940482No Hit
GACTCTTATCCATCCGTCCTCTCCCAGCTCACCAATCACTTGGCCTAGGC31490.10001156056146215No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)