FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001129338

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001129338
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2892081
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATCACTGCCAAGTTAGACAGCCCCAGGGTGACAGTGGCCCAGCTGGAC132060.45662621482593335No Hit
GCCGTAAAACCTCCAGTGACCCGTTTGCCTTCCCTCCTTCCCCCAGGTGA82490.2852271426699321No Hit
TGGTGATGGGTGTCGATGTTGGGGTCTGTGTGCCGGTGGGTGTTGGGGTT75830.26219874201310406No Hit
GGGAGCGTCACGTCTACCCGCCAGTTCCAGTCATAGCTGACTTGCAGTCC61980.2143093502567874No Hit
GGTCTCTTGCAGTCTGGAAGAGGACGGGCTCGTGGACAGAGGGCGTGGCC59220.20476604908368748No Hit
CCATGGCTATCCCCATCTGGGTCTGGCAGAAGTTCTCCTCCAGTTGTGCA49290.1704309111674258No Hit
CAACTTCTACCACCAACATCACCCCCAACCACACCAGTACAGGCACCAGA48880.16901324686272617No Hit
GGAGGATTCTGTGGCGTATGAAGACCACGGGATAACCGTATTGCCCAAGA48540.1678376228051704No Hit
CAGAGCCTGGAAATGGTTGAGACTACCCTGCCACGATTTTGCTCCCGCTC48030.16607418671883672No Hit
GCATGCACCCTCCTAACTGAGGTCTGGTGGGAGCCATGGAAGGACCAGGG46810.16185577098290124No Hit
CCTGATTCCTGTCCTCAGCTACCTGGCCAATGACATGGAGGAGGACGACG46650.16130253613228676No Hit
TCCTTGGGTCCATCGTGTAGCACCAGGGCCCATGGCTATCCCCATCTGGG45720.15808685856309004No Hit
GGTGAAGCAGTGTCCTTGTCCCAGGGGCTGCTGCTGTTGCTGCTGCTGAG44420.15359182540184732No Hit
CACAGATGGTGGTGTTGACGGTGATGCACACGGGGCAGCCCTCCTTCTCC42030.1453278798207934No Hit
CTGTGGATGCTGAGGAAGTGTCGGTGACAGGAAGAGGGGTGGTGTCACCT41050.14193931636077964No Hit
CCACTACGGTGACACCAACCCCAACACCCACCGGCACACAGACCCCAACA40870.14131692715383834No Hit
GGGCTGGACATCTTGAAGTGCCGCACCACAAAGTCCTTGCCGCCCATCAT40370.13958806824566808No Hit
CTCGCAGAAGAAGTGATCGATCTCGTGTGCACCGCAATATGGGAAGCTCA39270.13578457864769347No Hit
CGATATCCCACTCTCATGAGCTGGCAGCTGTGCCTGAGGATGACCATGTC39190.13550796122238623No Hit
CCACTACGGTGACACCAACCCCAACACCCACCGGCACACAGACCCCAACC38430.1328800956819674No Hit
GACTCTTATCCATCCGTCCTCTCCCAGCTCACCAATCACTTGGCCTAGGC38220.1321539749405359No Hit
GCCCCACCAAGCCAGATAGGAAGGCCACTGTTGGGAGGCAGGAGGGAAAG37480.1295952637564439No Hit
AAGGTGCTGACTTCCTTGGGAGATGCCATAAAGCACCTGGATGATCTCAA35480.12267982812376278No Hit
CGCCATGTTCTCAGATAAGAAGTACCTGACCGCCAGCCAGCTTGTTCCCC35250.12188455302600446No Hit
GGCGGCAGAATTCATCCTGTGGATGAGATGCAGGATGAGGGTGTATGAGC34880.12060519743395846No Hit
GGAAGACTCGGAGCAGGTAGGCACGAAGCACACAGGCTTGTTACAGACGG34880.12060519743395846No Hit
GATATGCTTTATGGTCTGCTCAGTCACCTCGTTCTTAATCCCTTTCTCAA34870.12057062025579504No Hit
CACAACCCCATGCTACCAGCAGTCTAGCTGCCAGCCGGATTGCTGCACCT34810.1203631571868146No Hit
AGGGTCACAGATCTTTAACAAGAATGGGGGCACTCAGGGTCTGAGGCCAC34680.11991365387069035No Hit
GGGATGTCATATGGGTCACACCCAGGGGGACGATGTCCCCAAAGCGCTGC33660.11638678169802298No Hit
CCTCTCCAGATCCCATGTGAATGACAGCCAGAATAACTCGAGCATAGGAA33520.1159027012037353No Hit
GACTCTTATCCATCCGTCCTCTCCCAGCTCACCAATCACTCGGCCTAGGC33500.1158335468474085No Hit
GTCTAAGATAATATCGCTTCTGATTCTCTTCTTCTCCATCTTCTTCATCT32930.11386264769209437No Hit
CTGTGATGATTTGGCTCCTGTGGCAAGACAGCTTGCTCCCAGGGAGAAGC32540.11251413774372157No Hit
GTGAAGACCAAGAACATGAAGATGATGGTGAAGATGAAGATGATGAAGAT32390.11199548007127047No Hit
GCTGAAATTGAACTCTGCTGTTGCTGTTGCTGTTGCTGTTGCTGCTGCTG32180.11126935932983897No Hit
CTGTGGATGCTGAGGAAGTGTCGGTGACAGGAAGAGGGGTGGTGTGACCT32170.11123478215167555No Hit
TTCATGGAACGGTATAACTGGAACCGACGCTTACGGAGCAAGGGTATGCG32160.11120020497351216No Hit
AAGCATTCAAGTAGCAGGTATTTCCCATATTCTGGAGCCCAGCCCCCACC31720.10967880913432232No Hit
GAGATTGGAGAAATAATATGCCATGGTATTACATAAGCTTTGGCTTCTCT31570.10916015146187122No Hit
GTGTCCAACAGGAGATCGACGACGTGATAGGGCAGGTGCGGCGACCAGAG31090.10750044691002776No Hit
TGTCACTAGCCCTTCCTCAGCATCCACAGGTCACGCCACCCCTCTTCCTG31000.10718925230655711No Hit
CGATGACAGCGCCTTCATGGAGCCGAGGTACCACGTCCGTCGAGAAGATC30310.10480342701328213No Hit
CACACTCGTGCAAGGCTTAGAAGGCCAGAAGATCATGTGTGTGGCTTGCG30250.10459596394430169No Hit
GGTAGAGCTGCAAGGACACCGCCTGCACAGGGTGCCCGGGGCTGGGCATT30020.10380068884654338No Hit
CATGCGTCTTCACCTGGAAGGGGTCCATGTGCCCCTCCTTCTGGCCACCA29820.10310914528327526No Hit
GGCTCACTCACCAGAGATGCGGCCTGCCACGTGCACTCCCATGGTGACTC29470.10189894404755606No Hit
GGTGGAGGTGAGGCAGATGCCCAGCAGGCGGCACACGTGGGGGTTGTCCA29360.10151859508775861No Hit
GCACAGGCTATGAGAAGTACAACTCCATGCGAGCTGACCCCGCGCTGTGC29090.10058501127734665No Hit
GCCAGATCAACACCCTCAACCACGGGGAGGTGGTGTGCGCTGTGACCATC29050.10044670256469303No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)