FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001129342

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001129342
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3075751
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATCACTGCCAAGTTAGACAGCCCCAGGGTGACAGTGGCCCAGCTGGAC180600.5871736691299133No Hit
GCCGTAAAACCTCCAGTGACCCGTTTGCCTTCCCTCCTTCCCCCAGGTGA82060.2667966295060946No Hit
TGGTGATGGGTGTCGATGTTGGGGTCTGTGTGCCGGTGGGTGTTGGGGTT69920.22732659438296532No Hit
GGGAGCGTCACGTCTACCCGCCAGTTCCAGTCATAGCTGACTTGCAGTCC69110.22469309121577138No Hit
GGTAGAGCTGCAAGGACACCGCCTGCACAGGGTGCCCGGGGCTGGGCATT61430.199723579704599No Hit
GACTCTTATCCATCCGTCCTCTCCCAGCTCACCAATCACTCGGCCTAGGC60480.19663490315048263No Hit
GCATGCACCCTCCTAACTGAGGTCTGGTGGGAGCCATGGAAGGACCAGGG55070.17904570298440933No Hit
GGTCTCTTGCAGTCTGGAAGAGGACGGGCTCGTGGACAGAGGGCGTGGCC54070.17579446450639208No Hit
CCTGATTCCTGTCCTCAGCTACCTGGCCAATGACATGGAGGAGGACGACG52190.16968213616771968No Hit
GGAGGATTCTGTGGCGTATGAAGACCACGGGATAACCGTATTGCCCAAGA47460.15430377816669816No Hit
GGTGAAGCAGTGTCCTTGTCCCAGGGGCTGCTGCTGTTGCTGCTGCTGAG47020.15287323323637056No Hit
GCCCCACCAAGCCAGATAGGAAGGCCACTGTTGGGAGGCAGGAGGGAAAG46530.1512801263821421No Hit
CTCGCAGAAGAAGTGATCGATCTCGTGTGCACCGCAATATGGGAAGCTCA42760.13902295732001713No Hit
CGATATCCCACTCTCATGAGCTGGCAGCTGTGCCTGAGGATGACCATGTC41960.13642196653760333No Hit
AAGGTGCTGACTTCCTTGGGAGATGCCATAAAGCACCTGGATGATCTCAA41340.13440619868123263No Hit
CTTGTCTCCCCAGATGTCAAGAATATGAACACGTGGCTGCTGTTCCTCCC41030.1333983147530473No Hit
CCACTACGGTGACACCAACCCCAACACCCACCGGCACACAGACCCCAACA40660.13219535651618095No Hit
CCACTACGGTGACACCAACCCCAACACCCACCGGCACACAGACCCCAACC39910.129756927657668No Hit
CCTCTCCAGATCCCATGTGAATGACAGCCAGAATAACTCGAGCATAGGAA38480.12510765663410334No Hit
CCATGGCTATCCCCATCTGGGTCTGGCAGAAGTTCTCCTCCAGTTGTGCA38310.12455494609284043No Hit
TCCTTGGGTCCATCGTGTAGCACCAGGGCCCATGGCTATCCCCATCTGGG38300.12452243370806024No Hit
CTGGAGGACCTGCTACGAAATAACAGAAGACCCAAGAAATGGGTGAGTGG38060.1237421364733361No Hit
AGGCAACAAACCTCAAGGTCCCCCACCTCCAGGAAAGCCACAAGGACCAC38050.12370962408855593No Hit
GGTAGAGCTGCAAGGACACCGCCTGCACAGGATGCCCGGGGCTGGGCATT37920.1232869630864137No Hit
CGATGACAGCGCCTTCATGGAGCCGAGGTACCACGTCCGTCGAGAAGATC37830.12299435162339213No Hit
CAGATGAGATCAAATTAACTCTAGTGTGCTCTGGCAGAGCCTTACCTCTC37500.12192144292564645No Hit
CTGTGGATGCTGAGGAAGTGTCGGTGACAGGAAGAGGGGTGGTGTCACCT37460.12179139338652577No Hit
CACAGATGGTGGTGTTGACGGTGATGCACACGGGGCAGCCCTCCTTCTCC37220.12101109615180161No Hit
ACCTGTCTGTCCTTTGTGTCCAGGAATTCCCCAGACAGTGGGGAGGGAGG36260.11788990721290507No Hit
GGCTCACTCACCAGAGATGCGGCCTGCCACGTGCACTCCCATGGTGACTC36180.1176298081346637No Hit
GGGATGTCATATGGGTCACACCCAGGGGGACGATGTCCCCAAAGCGCTGC35810.11642684989779731No Hit
GATATGCTTTATGGTCTGCTCAGTCACCTCGTTCTTAATCCCTTTCTCAA35560.115614040278293No Hit
AGGGTCACAGATCTTTAACAAGAATGGGGGCACTCAGGGTCTGAGGCCAC35080.11405344580884473No Hit
CAACTTCTACCACCAACATCACCCCCAACCACACCAGTACAGGCACCAGA35070.11402093342406457No Hit
CATGCGTCTTCACCTGGAAGGGGTCCATGTGCCCCTCCTTCTGGCCACCA34520.11223275226115509No Hit
AGAGCCCACACGATGAAGACCCCCAGGCAGTGACGTATGCCGAGGTGAAA33930.11031452155912491No Hit
GCTGAAATTGAACTCTGCTGTTGCTGTTGCTGTTGCTGTTGCTGCTGCTG33670.10946919955484041No Hit
GGACGTTGTTGCTGGACTCAGCCTTCTCGGTGGCAGTTCGGGTTCGGAAG33610.10927412524615938No Hit
CAGAGCCTGGAAATGGTTGAGACTACCCTGCCACGATTTTGCTCCCGCTC33510.10894900139835767No Hit
CTCTGCTCAACCTGTCTTGGCCCGAGGTGGATGAGGGTGCTGTCCTGGGC33430.1086889023201163No Hit
GGTGGAGGTGAGGCAGATGCCCAGCAGGCGGCACACGTGGGGGTTGTCCA33400.10859136516577576No Hit
GAGATTGGAGAAATAATATGCCATGGTATTACATAAGCTTTGGCTTCTCT32790.10660810969418526No Hit
AGCGTGAGGAAGTTGATGGGGAAGCCCAGCACGATCAGCAGAAACATGTA32400.10534012668775854No Hit
CTGGTATCTTGTCCTGTTCCTCCTTTTCCCTTTCCAGAGGCCAAGAAGAT32240.10481992853127577No Hit
TTCATGGAACGGTATAACTGGAACCGACGCTTACGGAGCAAGGGTATGCG32180.10462485422259474No Hit
GCACCAAGCCAAGAAAGTCCAGCAGGAGCACGAGCATCCCGATGATCACG32160.1045598294530344No Hit
GGTAGAGCTGCAAGGACACCACCTGCACAGGGTGCCCGGGGCTGGGCATT32150.10452731706825423No Hit
GGAAGACTCGGAGCAGGTAGGCACGAAGCACACAGGCTTGTTACAGACGG31720.10312928452270681No Hit
ACAAGGAGGCAACAAACCTCAAGGTCCCCCACCTCCAGGAAAGCCACAAG31710.10309677213792665No Hit
GTGTCCAACAGGAGATCGACGACGTGATAGGGCAGGTGCGGCGACCAGAG31670.10296672259880596No Hit
GGGCTGGACATCTTGAAGTGCCGCACCACAAAGTCCTTGCCGCCCATCAT31330.10186130151628009No Hit
AGGTAGATGATGCCACCTAGAGAGGCACCCAGAAGTGGTGCCACCACTGG30820.1002031698924913No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)