FastQCFastQC Report
Wed 8 May 2024
EGAF00001129351

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001129351
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3015795
Total Bases452.3 Mbp
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATCACTGCCAAGTTAGACAGCCCCAGGGTGACAGTGGCCCAGCTGGAC150200.49804446257122914No Hit
CGATGACAGCGCCTTCATGGAGCCGAGGTACCACGTCCGTCGAGAAGATC69910.231812838737381No Hit
TGGTGATGGGTGTCGATGTTGGGGTCTGTGTGCCGGTGGGTGTTGGGGTT58020.19238708201320048No Hit
GGGAGCGTCACGTCTACCCGCCAGTTCCAGTCATAGCTGACTTGCAGTCC53820.17846040596260687No Hit
GCCGTAAAACCTCCAGTGACCCGTTTGCCTTCCCTCCTTCCCCCAGGTGA49000.1624778872569256No Hit
GGTCTCTTGCAGTCTGGAAGAGGACGGGCTCGTGGACAGAGGGCGTGGCC48100.1594935995317984No Hit
GGTAGAGCTGCAAGGACACCGCCTGCACAGGGTGCCCGGGGCTGGGCATT46570.15442031039908216No Hit
GCATGCACCCTCCTAACTGAGGTCTGGTGGGAGCCATGGAAGGACCAGGG41690.13823883917839244No Hit
GACTCTTATCCATCCGTCCTCTCCCAGCTCACCAATCACTCGGCCTAGGC41510.137641981633367No Hit
CCATGGCTATCCCCATCTGGGTCTGGCAGAAGTTCTCCTCCAGTTGTGCA40560.1344919001457327No Hit
GCACCAAGCCAAGAAAGTCCAGCAGGAGCACGAGCATCCCGATGATCACG39680.13157392992560835No Hit
GGGCTGGACATCTTGAAGTGCCGCACCACAAAGTCCTTGCCGCCCATCAT39250.13014810356804757No Hit
AAGGTGCTGACTTCCTTGGGAGATGCCATAAAGCACCTGGATGATCTCAA39010.12935229350801364No Hit
GAGAACTCCTTTGTCCATTTGCACGTATGGATCTTTCCTCACTGCTTGAT37990.12597010075286946No Hit
GCCCCACCAAGCCAGATAGGAAGGCCACTGTTGGGAGGCAGGAGGGAAAG37660.12487586192032284No Hit
GGAGGATTCTGTGGCGTATGAAGACCACGGGATAACCGTATTGCCCAAGA37530.12444479813780447No Hit
GGTGAAGCAGTGTCCTTGTCCCAGGGGCTGCTGCTGTTGCTGCTGCTGAG37170.12325108304775358No Hit
AAGCATTCAAGTAGCAGGTATTTCCCATATTCTGGAGCCCAGCCCCCACC37150.12318476554275075No Hit
CCTCTCCAGATCCCATGTGAATGACAGCCAGAATAACTCGAGCATAGGAA35940.11917255649007974No Hit
GAGGTTTGTTGCCTCCTTGTGGGGGTGGTCCTTGTGGCTTTCCTGGAGGA35680.11831042892504297No Hit
GGCGGCAGAATTCATCCTGTGGATGAGATGCAGGATGAGGGTGTATGAGC35380.11731566635000058No Hit
CGCCATGTTCTCAGATAAGAAGTACCTGACCGCCAGCCAGCTTGTTCCCC35040.11618826876495252No Hit
GACTCTTATCCATCCGTCCTCTCCCAGCTCACCAATCACTTGGCCTAGGC34820.11545877620992144No Hit
CAGAGCCTGGAAATGGTTGAGACTACCCTGCCACGATTTTGCTCCCGCTC34730.1151603474374087No Hit
GGAAGACTCGGAGCAGGTAGGCACGAAGCACACAGGCTTGTTACAGACGG34450.11423190236736915No Hit
CTGTGATGATTTGGCTCCTGTGGCAAGACAGCTTGCTCCCAGGGAGAAGC33830.1121760597122815No Hit
CGGAGCATGTAGGTGTAGAAGGAAGCACCGAAGAGCAGCAGCACTATGAT33640.11154604341475464No Hit
ACAAGGAGGCAACAAACCTCAAGGTCCCCCACCTCCAGGAAAGCCACAAG33640.11154604341475464No Hit
CCTGATTCCTGTCCTCAGCTACCTGGCCAATGACATGGAGGAGGACGACG33550.11124761464224193No Hit
GGTAGAGCTGCAAGGACACCGCCTGCACAGGATGCCCGGGGCTGGGCATT33090.10972231202717692No Hit
GCTGGTTGCCTCCTTGTGGGGGTGGTCCTTGTGGCTTTCCTGGAGGAGAT32850.10892650196714299No Hit
AGGGTCACAGATCTTTAACAAGAATGGGGGCACTCAGGGTCTGAGGCCAC32740.10856175568962745No Hit
GGCTCACTCACCAGAGATGCGGCCTGCCACGTGCACTCCCATGGTGACTC32150.10660538929204405No Hit
GAGATTGGAGAAATAATATGCCATGGTATTACATAAGCTTTGGCTTCTCT31300.10378689532942391No Hit
CTCTTTTGCTTGTCCTTCTTGTTCATGTCAGTGTCCCTGAGCATGACGAT31200.10345530780440979No Hit
GGTGGAGGTGAGGCAGATGCCCAGCAGGCGGCACACGTGGGGGTTGTCCA31080.10305740277439283No Hit
TCCTTGGGTCCATCGTGTAGCACCAGGGCCCATGGCTATCCCCATCTGGG30890.10242738647686597No Hit
GGTAGAGCTGCAAGGACACCACCTGCACAGGGTGCCCGGGGCTGGGCATT30720.10186368768434194No Hit
GATATGCTTTATGGTCTGCTCAGTCACCTCGTTCTTAATCCCTTTCTCAA30210.10017259130676986No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)