FastQCFastQC Report
Thu 9 May 2024
EGAF00001129372

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001129372
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2581143
Total Bases387.1 Mbp
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCACTATCAAATGCTGTGAAGAGAAATGTACCCAGATGTATCATTATC236210.9151372085932472No Hit
GATGTCCTTCCATGGTGACTCTGGCTCTTGCAGGAGGTGGGCTACTGCAG136160.5275182351384639No Hit
GCTTTGTTGGCCTCAAATAGCTGCTTTATTAGATGCAATGAAGCAGGGGA130290.5047763723280733No Hit
GCTTTGTTGGCCTCAAATAGCTGCTTTATTAGATGTAATGAAGCAGGGGA122310.4738598365142885No Hit
GTATTTCATTATGTCCTGAGGAACAGTTGGTTATAGAAAGGCTACAAGGA98230.38056783370777986No Hit
AGTAAATACATTGGTTGAAGTTTAAAAGTCTATCCGTAGTAAACTTACAT98210.380490348655615No Hit
AGTAAATACATTGGTTGAAGTTTAAAAGTCTATGCGTAGTAAACTTACAT89620.3472105187508015No Hit
AGTGTGCCTTGGCTGAAAGCTGTGATCACCTGGCCAGGGTGGCCATCCCC76020.294520683278687No Hit
GGTTTGAAGACCACTTAAAAGCTTTGCCACATTCTTGACATTTGTAGGGT75570.29277726960497735No Hit
GGCATACAGTGGACGAGAAACAGTATATTCCAATGCATCCCTGCTGATCC74350.28805068142292No Hit
TTGCCTCCTTGGTATGAAACATCTTTCACACAACCCTCAATTGTAGGAGT70890.27464576739839675No Hit
ACTTTCACCCAAACCTCCTACACCCTGTTCGTCCGCGAGAACAACAGCCC60440.23415982764224996No Hit
CATCATGATCAACCAGTGAAGCCCCTGGATCGAGCAGTCTTCTGGATTGA57290.22195593192628227No Hit
AACAGAGAGAATTTTCACTATGGATCAGTGGTGACCTACCGCTGCAATCC56250.21792670921370882No Hit
GGTTGATATGGGAGTAAAGACCTTGGCATGGTACATACCCCTCATGCAAG52040.20161610573300276No Hit
CCTGAAGTCTGGCTATGGAAAATATCTTGGTATAAATTCAGATGAACTTG50770.19669580492053326No Hit
CTGCTGTCCAGACCATATTGACTTTCCATTTGCCCTACTGTGGACCCAAC50320.1949523912468236No Hit
GTAGGTGTCTTCTCCTCGATCCCTGGGATGGGAGCCTTGTGGGGTAGTAT46310.17941663828776633No Hit
GGATACTCATTCTACCCAGTGTCACGAGAAATGAAACAGGACCCTATCAA45880.1777507096662215No Hit
GGAGGAAGAAGATCTGCACACCACAGCCAGCGCGGGAGATGGCCTTATTT45790.17740202693147958No Hit
GCCCACAGCATCCTTACCTGCTGTGTGACTCGTTCTGGGTACCATCTCCC45570.17654969135766596No Hit
TGTGTGAATGCAGTCATCTGTATAAAATGTCATCAATACCTTGATCATTT42920.16628292194582012No Hit
GGAGGAAGAAGATCTGCACACCACAGCCAGCGCGGGAGATGGCCTTACTT42870.16608920931540794No Hit
CTCAGTCCATTGCCCCAAGGCTCACTTGTTAGCTTGCCTGGGGAAAGGGG42610.16508190363726458No Hit
GTGTGATCTGAACCCTTTGCTCAACCTCGCCTGCACTGACACCCATATGC39590.15338166076036855No Hit
CTCCATCATAGGACCCAAAAGGGGGGAACATGCCTGGACCCACAGACTGC38930.15082465403892772No Hit
CCGCATTAATATCCCAGAGAGAGCAGGTGGACCAGAAGGCAACTTAAACC38840.15047597130418577No Hit
CTGTCCTAATTCCCCACTCCTCTAGGCAGGGCCAGCATTTCTGCGGGGGG38380.1486938151043937No Hit
GAAATATCCAGTTACTCCTCTAGTCCCATCACCTCGCTTTATGATGTGTA38080.14753153932192056No Hit
GTCAAAGGAGTGTTGGAGTCAACAGGAGTTGTTGAAAGGGTGCTAGCCTC36520.14148770525306037No Hit
GAACACCCCACACATTAGGGCGGCTGCCTTGTCCTTCTGGGAGGAGCTGG36200.14024794441842237No Hit
GCAGGTACCCTCAACTGAGTAAGGGACAATGAACAGCCTCTTGGCCATTC35380.13707105727966254No Hit
AGGCCCTCATGAATGACCTCTTTCTTTCCTTTCTTCTTTTCTAGCTACTC34600.13404914024523243No Hit
ACGTCAGCCCCAGGAAATGCCCGGTTTGGCCAGGGTTCAGGACCCATTGT34480.1335842299322432No Hit
GGAGAACCAGAAAATGATGAACCAAGATGCCAGAACCAGGAGGCCACAGA34340.13304183456708907No Hit
GGCAGTTCCACCATCAAGTACACCACCACCTCCTCCTCCAGCAGGAAGAG34180.1324219541497701No Hit
AAGGCACACTTACCACTGTTTTCTTGGCTCCAGTCCAAACCTGAGAGTAG33350.1292063244849278No Hit
GGCAAGTGGAAAGCCTTCTCCACCTGTGGCTTATACCTCACTGTGGTGTC32780.12699800049822887No Hit
CCAGGTCTATATGAGAAACCTTCTCTCTCAGCCCAGCCGGGCCCCACGGT32620.1263781200809099No Hit
GACCTGCGCTGTGAACTGCCCAAGCTGGAGAAGCGGCTGCGTGCCACGGC31600.1224263824205013No Hit
GATCCACCTCCTGCTGGTAGCGCTTACGGTCCCGCATGGCGTTCTCCTTG31230.12099290895545113No Hit
TGGAGGCGTGCATTTGTTAGGTATAATGCACTGAGGGGCGGGGCCGCTCC31220.12095416642936868No Hit
AGAGAACCCTTACAAATTCGAAGAATGTGGTAAAGCCTTTTATTGGTTTT31210.12091542390328626No Hit
GGATGACCTCATGCTCATCAAGCTGGCTAAGCCTGCCATGCTCAATCCCA30950.11990811822514288No Hit
AGTGTGCCTTGGCTGAAAGCTGTGATCACCCGGCCAGGGTGGCCATCCCC30690.11890081254699954No Hit
GTTGCTGCTTCAGGAATGGAGGGAGTGAGAGCCTTTTCCGACAATGCCGA30330.11750608160803178No Hit
GGAACCCAGTCACATCCAAAGAGTGGTACTGGAACCAGGTGAGGTCGTGG29890.11580141046040457No Hit
GCTTTGTATATGAGGCCTGTGGGGCAAGAACCTTTGTGCAGCGTTTCCGC29820.11553021277782749No Hit
AGGTGACACCATGCCTCTTCCTGTCACTAGCCCTTCCTCAGCATCCACAG29740.115220272569168No Hit
CCTGCATATAGTGGACGAGAAACAGCATATTCCAATGCATCCCTGCTGAT29530.11440667952143681No Hit
CCACCTCTCCTTTGTAGACATTTGCTATTCTTCCAATGTTACTCCAAATA29330.11363182899978809No Hit
GGTTGTCTACCTGAGCTACATGGCGGTGGAGTACAATGTGTCCTTCCCCC29140.11289572100422177No Hit
CATCTAATTTCCCGGGCCATACCCTGCAGGTACTGGATGATGCTGAGGTT28990.11231458311298521No Hit
AACAGAGAGAATTTTCACTATGGATCAGTGGTGACCTACCGCTGCAATCT28950.11215961300865547No Hit
CATGCGTCTTCACCTGGAAGGGGTCCATGTGCCCCTCCTTCTGGCCACCA28820.11165596016958379No Hit
TGGAGCCAGACCATAAATACAAGATGAACCTGTACGGCTTCCACGGTGGC28450.1102224867045336No Hit
AAGTGGTGACATCAGAACCTGCCCTCCACACCCAACAGCTGGACTGCAGC28230.10937015113072No Hit
TGTGTGAATGCAGTCATCTGTATAAAATGTCATCATTACCTTCATCATTT28090.10882775576556587No Hit
GAGAAATTATTTAATACTTCAGCCAAGAAGTCTTTCTAGACTAAATTGTT27690.1072780547222684No Hit
ATGGCATTGTATGAGAGCATGAGGGAACGCAGGTTGGACAGGTGGGCAAA27640.1070843420918562No Hit
GCCTTGAGCAGCTGGTACGACAGCCAGGCGTTCTGGCCCGAGTCGCCGTC27450.10634823409628989No Hit
GTCACATGTGATCCAGGTCTCCTCCGAGGGGCTGGCCAGTCTCTTCTCCC27200.10537967094422897No Hit
CTGAGGAGCAGTAAGGGATGGCATCAGCAACGCGAGCCACAGGCAGGCCT27190.10534092841814655No Hit
CGCCCCTCAGTGCATTATACCTAACAAATGCACGCCTCCAAATGTGGAAA26980.10452733537041536No Hit
GGGATGAGTGGAAGGTTCTCTTACGGAGTTACTCGTCATCTTCCTCAGGG26940.1043723652660856No Hit
TGAAAAGCAAGGCTGTATAGAAGACGGTGACCACGGTGAGGTGAGAGGCG26900.10421739516175586No Hit
GCAGGGAGCCTACGTTCATGGGCCTCCCCTTCCCTGGATAGATGGTACAT26840.10398494000526123No Hit
CAACCCTGCATCTTTAGACAAGGCCATGTCCATTCCCATGTGGGTGGACA26100.1011179930751609No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)