FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001129380

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001129380
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2329390
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATCACTGCCAAGTTAGACAGCCCCAGGGTGACAGTGGCCCAGCTGGAC110800.475661009963982No Hit
GCCGTAAAACCTCCAGTGACCCGTTTGCCTTCCCTCCTTCCCCCAGGTGA81220.34867497499345323No Hit
GGGAGCGTCACGTCTACCCGCCAGTTCCAGTCATAGCTGACTTGCAGTCC66770.2866415671055513No Hit
TGGTGATGGGTGTCGATGTTGGGGTCTGTGTGCCGGTGGGTGTTGGGGTT55280.23731534865351014No Hit
GGTCTCTTGCAGTCTGGAAGAGGACGGGCTCGTGGACAGAGGGCGTGGCC46220.1984210458532062No Hit
GGTGAAGCAGTGTCCTTGTCCCAGGGGCTGCTGCTGTTGCTGCTGCTGAG41900.17987541802789572No Hit
CCTGATTCCTGTCCTCAGCTACCTGGCCAATGACATGGAGGAGGACGACG41060.17626932372852977No Hit
CTGTGGATGCTGAGGAAGTGTCGGTGACAGGAAGAGGGGTGGTGTCACCT36950.1586252194780608No Hit
CAACTTCTACCACCAACATCACCCCCAACCACACCAGTACAGGCACCAGA35820.1537741640515328No Hit
GCCCCACCAAGCCAGATAGGAAGGCCACTGTTGGGAGGCAGGAGGGAAAG35750.15347365619325232No Hit
GGAGGATTCTGTGGCGTATGAAGACCACGGGATAACCGTATTGCCCAAGA34870.1496958431177261No Hit
CGATGACAGCGCCTTCATGGAGCCGAGGTACCACGTCCGTCGAGAAGATC34490.1480645147442034No Hit
GACTCTTATCCATCCGTCCTCTCCCAGCTCACCAATCACTCGGCCTAGGC33950.1457463112660396No Hit
GCATGCACCCTCCTAACTGAGGTCTGGTGGGAGCCATGGAAGGACCAGGG32600.13995080257063008No Hit
CACAACCCCATGCTACCAGCAGTCTAGCTGCCAGCCGGATTGCTGCTCCT31230.13406943448714043No Hit
AAGGTGCTGACTTCCTTGGGAGATGCCATAAAGCACCTGGATGATCTCAA30180.129561816612933No Hit
GTGTCCAACAGGAGATCGACGACGTGATAGGGCAGGTGCGGCGACCAGAG29770.12780169915729012No Hit
CACAGATGGTGGTGTTGACGGTGATGCACACGGGGCAGCCCTCCTTCTCC29740.1276729100751699No Hit
GGAAGACTCGGAGCAGGTAGGCACGAAGCACACAGGCTTGTTACAGACGG29600.1270718943586089No Hit
CGATATCCCACTCTCATGAGCTGGCAGCTGTGCCTGAGGATGACCATGTC29490.12659966772416814No Hit
ACCTGTCTGTCCTTTGTGTCCAGGAATTCCCCAGACAGTGGGGAGGGAGG29490.12659966772416814No Hit
GGCTCACTCACCAGAGATGCGGCCTGCCACGTGCACTCCCATGGTGACTC29440.12638501925396778No Hit
GATATGCTTTATGGTCTGCTCAGTCACCTCGTTCTTAATCCCTTTCTCAA28480.122263768626121No Hit
CCATGGCTATCCCCATCTGGGTCTGGCAGAAGTTCTCCTCCAGTTGTGCA28350.12170568260360008No Hit
GGTAGAGCTGCAAGGACACCGCCTGCACAGGATGCCCGGGGCTGGGCATT27430.11775615075191358No Hit
CTGTGATGATTTGGCTCCTGTGGCAAGACAGCTTGCTCCCAGGGAGAAGC27400.11762736166979339No Hit
CTGGTATCTTGTCCTGTTCCTCCTTTTCCCTTTCCAGAGGCCAAGAAGAT27350.11741271319959304No Hit
CTCTGCTCAACCTGTCTTGGCCCGAGGTGGATGAGGGTGCTGTCCTGGGC27300.11719806472939268No Hit
CTCGCAGAAGAAGTGATCGATCTCGTGTGCACCGCAATATGGGAAGCTCA27100.11633947084859127No Hit
AAGCATTCAAGTAGCAGGTATTTCCCATATTCTGGAGCCCAGCCCCCACC26720.1147081424750686No Hit
GGTAGAGCTGCAAGGACACCACCTGCACAGGATGCCTGGGGCTGGCCATT26480.11367782981810688No Hit
CATGCGTCTTCACCTGGAAGGGGTCCATGTGCCCCTCCTTCTGGCCACCA26360.11316267348962603No Hit
TTCATGGAACGGTATAACTGGAACCGACGCTTACGGAGCAAGGGTATGCG26330.11303388440750584No Hit
GAGATTGGAGAAATAATATGCCATGGTATTACATAAGCTTTGGCTTCTCT25550.10968536827238032No Hit
GGTGGAGGTGAGGCAGATGCCCAGCAGGCGGCACACGTGGGGGTTGTCCA25440.10921314163793955No Hit
GCTGAAATTGAACTCTGCTGTTGCTGTTGCTGTTGCTGTTGCTGCTGCTG25440.10921314163793955No Hit
GTTCTCTGTTGCATGCTTCTCCTTCTCCACCTTGGCCAGTGTCAGCTCCA25080.107667672652497No Hit
CAGATGAGATCAAATTAACTCTAGTGTGCTCTGGCAGAGCCTTACCTCTC24910.10693786785381582No Hit
GGACGTTGTTGCTGGACTCAGCCTTCTCGGTGGCAGTTCGGGTTCGGAAG24610.1056499770326137No Hit
CCACCCCTAACATGCTATTGACTTGCCTTCTCCCTTCCCATCTCCACTCC24580.10552118795049348No Hit
CTGTGGATGCTGAGGAAGTGTCGGTGACAGGAAGAGGGGTGGTGTGACCT24450.10496310192797256No Hit
CTGTGGATGCTGAGGAAGTGTCGGTGACAGGAAGAGGGGTGGCGTGACCT24400.10474845345777221No Hit
CCACTACGGTGACACCAACCCCAACACCCACCGGCACACAGACCCCAACC24320.10440501590545165No Hit
AGAGCCCACACGATGAAGACCCCCAGGCAGTGACGTATGCCGAGGTGAAA24160.1037181408008105No Hit
GTGTGATCTTCTGGCCTTCTAAGCCTTGCACGAGTGTGGGCTTCCTGTTA23970.10290247661404917No Hit
GGTAGAGCTGCAAGGACACCACCTGCACAGGGTGCCCGGGGCTGGGCATT23960.1028595469200091No Hit
GAGAACTCCTTTGTCCATTTGCACGTATGGATCTTTCCTCACTGCTTGAT23850.10238732028556832No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)