FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001129381

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001129381
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2329390
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATCACTGCCAAGTTAGACAGCCCCAGGGTGACAGTGGCCCAGCTGGAC107230.4603351091916768No Hit
GGGAGCGTCACGTCTACCCGCCAGTTCCAGTCATAGCTGACTTGCAGTCC76410.32802579216017924No Hit
GCCGTAAAACCTCCAGTGACCCGTTTGCCTTCCCTCCTTCCCCCAGGTGA66950.2874143015982725No Hit
TGGTGATGGGTGTCGATGTTGGGGTCTGTGTGCCGGTGGGTGTTGGGGTT48090.2064488986386994No Hit
GGTGAAGCAGTGTCCTTGTCCCAGGGGCTGCTGCTGTTGCTGCTGCTGAG40970.17588295648216914No Hit
CGATGACAGCGCCTTCATGGAGCCGAGGTACCACGTCCGTCGAGAAGATC40620.1743804171907667No Hit
GGTCTCTTGCAGTCTGGAAGAGGACGGGCTCGTGGACAGAGGGCGTGGCC40000.1717187761602823No Hit
GGAGGATTCTGTGGCGTATGAAGACCACGGGATAACCGTATTGCCCAAGA37510.16102928234430475No Hit
CCTGATTCCTGTCCTCAGCTACCTGGCCAATGACATGGAGGAGGACGACG35160.15094080424488815No Hit
AAGGTGCTGACTTCCTTGGGAGATGCCATAAAGCACCTGGATGATCTCAA33310.1429988108474751No Hit
GACTCTTATCCATCCGTCCTCTCCCAGCTCACCAATCACTCGGCCTAGGC32560.1397790837944698No Hit
GGAAGACTCGGAGCAGGTAGGCACGAAGCACACAGGCTTGTTACAGACGG31660.13591541133086343No Hit
GCCCCACCAAGCCAGATAGGAAGGCCACTGTTGGGAGGCAGGAGGGAAAG31280.13428408295734076No Hit
GGCTCACTCACCAGAGATGCGGCCTGCCACGTGCACTCCCATGGTGACTC30660.13162244192685638No Hit
GCATGCACCCTCCTAACTGAGGTCTGGTGGGAGCCATGGAAGGACCAGGG30400.13050626988181455No Hit
CAACTTCTACCACCAACATCACCCCCAACCACACCAGTACAGGCACCAGA29900.128359785179811No Hit
CTCTGCTCAACCTGTCTTGGCCCGAGGTGGATGAGGGTGCTGTCCTGGGC29630.1272006834407291No Hit
AAGCATTCAAGTAGCAGGTATTTCCCATATTCTGGAGCCCAGCCCCCACC29190.125311776902966No Hit
GAGAACTCCTTTGTCCATTTGCACGTATGGATCTTTCCTCACTGCTTGAT28850.12385216730560361No Hit
AGAGCCCACACGATGAAGACCCCCAGGCAGTGACGTATGCCGAGGTGAAA28790.12359458914136319No Hit
CACAGATGGTGGTGTTGACGGTGATGCACACGGGGCAGCCCTCCTTCTCC28590.12273599526056178No Hit
CACAACCCCATGCTACCAGCAGTCTAGCTGCCAGCCGGATTGCTGCTCCT28360.12174861229764014No Hit
GGTAGAGCTGCAAGGACACCGCCTGCACAGGATGCCCGGGGCTGGGCATT28300.12149103413339973No Hit
CTGTGGATGCTGAGGAAGTGTCGGTGACAGGAAGAGGGGTGGTGTCACCT28230.12119052627511924No Hit
CTCGCAGAAGAAGTGATCGATCTCGTGTGCACCGCAATATGGGAAGCTCA27290.1171551350353526No Hit
CGATATCCCACTCTCATGAGCTGGCAGCTGTGCCTGAGGATGACCATGTC27120.1164253302366714No Hit
GAGGATGAGGAGGAGATGAACGCGGAGCTCACTGCCAAGAAGCGCAAGCT26880.11539501757970971No Hit
GTGTCCAACAGGAGATCGACGACGTGATAGGGCAGGTGCGGCGACCAGAG26800.11505158002738913No Hit
CCACTACGGTGACACCAACCCCAACACCCACCGGCACACAGACCCCAACA26770.11492279094526894No Hit
GATATGCTTTATGGTCTGCTCAGTCACCTCGTTCTTAATCCCTTTCTCAA26240.11264751716114518No Hit
CAGATGAGATCAAATTAACTCTAGTGTGCTCTGGCAGAGCCTTACCTCTC26070.11191771236246399No Hit
GGTGGAGGTGAGGCAGATGCCCAGCAGGCGGCACACGTGGGGGTTGTCCA25990.11157427481014341No Hit
CACACTCGTGCAAGGCTTAGAAGGCCAGAAGATCACACGCGTGGCTTGTG25890.11114497786974273No Hit
GGAACCCCAGAAAAGACCTTGCCCCTTCTGTCCCTGCAGATGACGTCCAC25680.11024345429490123No Hit
CCACCCCTAACATGCTATTGACTTGCCTTCTCCCTTCCCATCTCCACTCC25610.10994294643662074No Hit
GGTAGAGCTGCAAGGACACCACCTGCACAGGATGCCTGGGGCTGGCCATT25610.10994294643662074No Hit
CATGCGTCTTCACCTGGAAGGGGTCCATGTGCCCCTCCTTCTGGCCACCA24950.10710958662997609No Hit
CTGTGATGATTTGGCTCCTGTGGCAAGACAGCTTGCTCCCAGGGAGAAGC24820.10655150060745516No Hit
ACCTGTCTGTCCTTTGTGTCCAGGAATTCCCCAGACAGTGGGGAGGGAGG24800.10646564121937502No Hit
GAACCAGCAGATGCCTTCTGTTTTGCCTTCCCAGAACAAGCCTTCTCTCC24720.10612220366705445No Hit
GTGTGATCTTCTGGCCTTCTAAGCCTTGCACGAGTGTGGGCTTCCTGTTA24360.10457673468161192No Hit
GCACCAAGCCAAGAAAGTCCAGCAGGAGCACGAGCATCCCGATGATCACG24170.10376107049485059No Hit
TTCATGGAACGGTATAACTGGAACCGACGCTTACGGAGCAAGGGTATGCG24040.10320298447232966No Hit
CCATGGCTATCCCCATCTGGGTCTGGCAGAAGTTCTCCTCCAGTTGTGCA23940.10277368753192895No Hit
GGTAGAGCTGCAAGGACACCACCTGCACAGGGTGCCCGGGGCTGGGCATT23840.10234439059152826No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)