FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001129382

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001129382
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3689314
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATCACTGCCAAGTTAGACAGCCCCAGGGTGACAGTGGCCCAGCTGGAC142060.3850580351794398No Hit
GCCGTAAAACCTCCAGTGACCCGTTTGCCTTCCCTCCTTCCCCCAGGTGA98510.2670144097249516No Hit
GGGAGCGTCACGTCTACCCGCCAGTTCCAGTCATAGCTGACTTGCAGTCC81500.2209082772569643No Hit
CCTGATTCCTGTCCTCAGCTACCTGGCCAATGACATGGAGGAGGACGACG80020.2168966913632182No Hit
GGTGAAGCAGTGTCCTTGTCCCAGGGGCTGCTGCTGTTGCTGCTGCTGAG74350.2015279805405558No Hit
GGTCTCTTGCAGTCTGGAAGAGGACGGGCTCGTGGACAGAGGGCGTGGCC69990.18971006534006052No Hit
CGATATCCCACTCTCATGAGCTGGCAGCTGTGCCTGAGGATGACCATGTC63020.17081766420532382No Hit
TGGTGATGGGTGTCGATGTTGGGGTCTGTGTGCCGGTGGGTGTTGGGGTT62720.17000450490253743No Hit
CTGGTATCTTGTCCTGTTCCTCCTTTTCCCTTTCCAGAGGCCAAGAAGAT57240.1551507949716397No Hit
CTCGCAGAAGAAGTGATCGATCTCGTGTGCACCGCAATATGGGAAGCTCA56330.15268421175318772No Hit
CACAACCCCATGCTACCAGCAGTCTAGCTGCCAGCCGGATTGCTGCTCCT55740.15108499845770787No Hit
CAACTTCTACCACCAACATCACCCCCAACCACACCAGTACAGGCACCAGA55550.15056999756594316No Hit
GGAAGACTCGGAGCAGGTAGGCACGAAGCACACAGGCTTGTTACAGACGG55430.1502447338448286No Hit
GCCCCACCAAGCCAGATAGGAAGGCCACTGTTGGGAGGCAGGAGGGAAAG55300.14989236481362117No Hit
CCATGGCTATCCCCATCTGGGTCTGGCAGAAGTTCTCCTCCAGTTGTGCA55040.1491876267512063No Hit
GACTCTTATCCATCCGTCCTCTCCCAGCTCACCAATCACTCGGCCTAGGC54370.14737157097498343No Hit
GAGATTGGAGAAATAATATGCCATGGTATTACATAAGCTTTGGCTTCTCT53960.1462602532611754No Hit
CACAGATGGTGGTGTTGACGGTGATGCACACGGGGCAGCCCTCCTTCTCC53320.14452551341523112No Hit
TCCTTGGGTCCATCGTGTAGCACCAGGGCCCATGGCTATCCCCATCTGGG52440.14214024612705778No Hit
CTTGTCTCCCCAGATGTCAAGAATATGAACACGTGGCTGCTGTTCCTCCC52230.1415710346151073No Hit
GCATGCACCCTCCTAACTGAGGTCTGGTGGGAGCCATGGAAGGACCAGGG52050.1410831390334355No Hit
CATGCGTCTTCACCTGGAAGGGGTCCATGTGCCCCTCCTTCTGGCCACCA51590.13983629476916304No Hit
GGTGGAGGTGAGGCAGATGCCCAGCAGGCGGCACACGTGGGGGTTGTCCA50990.13820997616359032No Hit
GGAGGATTCTGTGGCGTATGAAGACCACGGGATAACCGTATTGCCCAAGA49660.13460496992123738No Hit
CGATGACAGCGCCTTCATGGAGCCGAGGTACCACGTCCGTCGAGAAGATC48940.1326533875945501No Hit
AGGGTCACAGATCTTTAACAAGAATGGGGGCACTCAGGGTCTGAGGCCAC46750.1267173246842096No Hit
GTGTCCAACAGGAGATCGACGACGTGATAGGGCAGGTGCGGCGACCAGAG46170.12514521669882261No Hit
GATATGCTTTATGGTCTGCTCAGTCACCTCGTTCTTAATCCCTTTCTCAA45940.12452179456668638No Hit
AAGGTGCTGACTTCCTTGGGAGATGCCATAAAGCACCTGGATGATCTCAA45670.12378995119417865No Hit
TTCATGGAACGGTATAACTGGAACCGACGCTTACGGAGCAAGGGTATGCG44730.12124205204544801No Hit
CAGAGCCTGGAAATGGTTGAGACTACCCTGCCACGATTTTGCTCCCGCTC44470.12053731398303316No Hit
GTTCTCTGTTGCATGCTTCTCCTTCTCCACCTTGGCCAGTGTCAGCTCCA44150.11966994406006103No Hit
GAGAACTCCTTTGTCCATTTGCACGTATGGATCTTTCCTCACTGCTTGAT44020.11931757502885361No Hit
AGGCTACTGAGGTGGTCAACCCTCTCCTGGAGCCTGTGGAGCACCGAGAT43760.11861283696643875No Hit
AGGCAACAAACCTCAAGGTCCCCCACCTCCAGGAAAGCCACAAGGACCAC41590.112730984676284No Hit
CCTGATTCCTGTCCTCAGCTATCTGGCCAATGACATGGAGGAGGACGACG41140.11151124572210444No Hit
ACCTGTCTGTCCTTTGTGTCCAGGAATTCCCCAGACAGTGGGGAGGGAGG41020.11118598200098989No Hit
GAGGATGAGGAGGAGATGAACGCGGAGCTCACTGCCAAGAAGCGCAAGCT40800.11058966517894654No Hit
CCCCACGATGTCAGAAGGTGCCACAGTCTCATGGAGCATATAATACTGCA40690.11029150676792487No Hit
AGAGCCCACACGATGAAGACCCCCAGGCAGTGACGTATGCCGAGGTGAAA40180.10890913595318806No Hit
AATGTCCTTCTTACCCGTCACCACCATCTGCTAGCATGTCTGTTCCTCTT40160.10885492533300228No Hit
GTGAAGACCAAGAACATGAAGATGATGGTGAAGATGAAGATGATGAAGAT40100.10869229347244501No Hit
GGTAGCCTCATGACAGGAGGACAGGGGAGGTTGGCAGACATACGGATGAG39450.10693044831640787No Hit
CCACCCCTAACATGCTATTGACTTGCCTTCTCCCTTCCCATCTCCACTCC38910.10546676157139241No Hit
GGACGTTGTTGCTGGACTCAGCCTTCTCGGTGGCAGTTCGGGTTCGGAAG38830.10524991909064936No Hit
GCACCAAGCCAAGAAAGTCCAGCAGGAGCACGAGCATCCCGATGATCACG38640.10473491819888467No Hit
GCCCATTCCAGGATGAAGATGGAAAGGAACTGTCAGACGAGGATATCCGA38630.10470781288879179No Hit
GCTGAAATTGAACTCTGCTGTTGCTGTTGCTGTTGCTGTTGCTGCTGCTG38360.10397596951628406No Hit
GGTTTTCTGCACTGCTTGTAAAACAGACTTGTAGATGCGGAAACTGATGG38000.10300017835294041No Hit
GCACTGCTGCTGTTGATAAGACATCCTGCTGGAGTCTCAGGATCTGAAAG37700.10218701905015404No Hit
GCACAGGCTATGAGAAGTACAACTCCATGCGAGCTGACCCCGCGCTGTGC37700.10218701905015404No Hit
GAGTCGCACAGCTCAGCCTGTCCCTGACATGTCTCTGTGCTTTGTAGGCG37610.10194307125931813No Hit
GGAAAATGCTGGCTGACCTAAAGCCACCTCCTTACTTTGCCTCCTTCTGC37010.1003167526537454No Hit
CTTGGTCTCCTTCACATGTGTGGGCTATGCGGTCACCAAGGCCCACCCTG36990.10026254203355962No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)