FastQCFastQC Report
Wed 8 May 2024
EGAF00001129386

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001129386
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2328264
Total Bases349.2 Mbp
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTTGTTGGCCTCAAATAGCTGCTTTATTAGATGCAATGAAGCAGGGGA140370.6028955479275546No Hit
GCTTTGTTGGCCTCAAATAGCTGCTTTATTAGATGTAATGAAGCAGGGGA133340.5727013775070181No Hit
AGCCACTATCAAATGCTGTGAAGAGAAATGTACCCAGATGTATCATTATC121740.5228788487903434No Hit
GATGTCCTTCCATGGTGACTCTGGCTCTTGCAGGAGGTGGGCTACTGCAG108010.4639078729903482No Hit
AGTGTGCCTTGGCTGAAAGCTGTGATCACCTGGCCAGGGTGGCCATCCCC84220.3617287386653747No Hit
GGAGGAAGAAGATCTGCACACCACAGCCAGCGCGGGAGATGGCCTTATTT69730.29949352822532155No Hit
AACAGAGAGAATTTTCACTATGGATCAGTGGTGACCTACCGCTGCAATCC63980.2747970161459353No Hit
GGCATACAGTGGACGAGAAACAGTATATTCCAATGCATCCCTGCTGATCC63460.272563592444843No Hit
AGTAAATACATTGGTTGAAGTTTAAAAGTCTATCCGTAGTAAACTTACAT56350.24202581837798462No Hit
CACAACCAGGAAGCTTATTGGACTGTCCTCAGAGATCTGCACCCTATAGA50910.21866077042809579No Hit
GGTTTGAAGACCACTTAAAAGCTTTGCCACATTCTTGACATTTGTAGGGT50160.215439486243828No Hit
GCCCACAGCATCCTTACCTGCTGTGTGACTCGTTCTGGGTACCATCTCCC49330.21187459841323836No Hit
AGGCCCTCATGAATGACCTCTTTCTTTCCTTTCTTCTTTTCTAGCTACTC48060.20641989052787826No Hit
CATGCGTCTTCACCTGGAAGGGGTCCATGTGCCCCTCCTTCTGGCCACCA47380.2034992595341422No Hit
CTGCTGTCCAGACCATATTGACTTTCCATTTGCCCTACTGTGGACCCAAC47230.20285500269728862No Hit
GTATTTCATTATGTCCTGAGGAACAGTTGGTTATAGAAAGGCTACAAGGA46890.20139468720042059No Hit
TGTGTGAATGCAGTCATCTGTATAAAATGTCATCAATACCTTGATCATTT46420.19937601577827946No Hit
GTAGGTGTCTTCTCCTCGATCCCTGGGATGGGAGCCTTGTGGGGTAGTAT44680.19190263647077824No Hit
ACTTTCACCCAAACCTCCTACACCCTGTTCGTCCGCGAGAACAACAGCCC43730.18782234317070573No Hit
GTCAAAGGAGTGTTGGAGTCAACAGGAGTTGTTGAAAGGGTGCTAGCCTC43270.18584662220435483No Hit
AGTAAATACATTGGTTGAAGTTTAAAAGTCTATGCGTAGTAAACTTACAT41090.1764834228420832No Hit
GACCTGCGCTGTGAACTGCCCAAGCTGGAGAAGCGGCTGCGTGCCACGGC40700.17480835506626397No Hit
GGTGGAGGTGAGGCAGATGCCCAGCAGGCGGCACACGTGGGGGTTGTCCA40470.17382049458308851No Hit
GATCCACCTCCTGCTGGTAGCGCTTACGGTCCCGCATGGCGTTCTCCTTG39710.17055625994303053No Hit
CTGACTATCACAGCGAGTACCACCAAGGTGATCACCACCCCAGTGAGTAC38330.16462909704397782No Hit
GGAAAATGCTGGCTGACCTAAAGCCACCTCCTTACTTTGCCTCCTTCTGC38300.16450024567660712No Hit
CATCATGATCAACCAGTGAAGCCCCTGGATCGAGCAGTCTTCTGGATTGA37870.16265337607762692No Hit
GGAGGAAGAAGATCTGCACACCACAGCCAGCGCGGGAGATGGCCTTACTT37700.1619232183291929No Hit
GGATACTCATTCTACCCAGTGTCACGAGAAATGAAACAGGACCCTATCAA37600.16149371377129054No Hit
GAACACCCCACACATTAGGGCGGCTGCCTTGTCCTTCTGGGAGGAGCTGG37420.1607206055670663No Hit
CTCAGTCCATTGCCCCAAGGCTCACTTGTTAGCTTGCCTGGGGAAAGGGG35240.15135740620479463No Hit
TGGAGGCGTGCATTTGTTAGGTATAATGCACTGAGGGGCGGGGCCGCTCC34980.15024069435424847No Hit
AAGTGGTGACATCAGAACCTGCCCTCCACACCCAACAGCTGGACTGCAGC34400.14774956791841473No Hit
CTGTCCTAATTCCCCACTCCTCTAGGCAGGGCCAGCATTTCTGCGGGGGG34330.1474489147278831No Hit
AACAGAGAGAATTTTCACTATGGATCAGTGGTGACCTACCGCTGCAATCT33410.1434974727951813No Hit
TGTGTGAATGCAGTCATCTGTATAAAATGTCATCATTACCTTCATCATTT33340.14319681960464964No Hit
GCCTTCACCCAAACCTCCTACACCCTGTTCGTCCGCGAGAACAACAGCCC32890.141264049094089No Hit
GTCACATGTGATCCAGGTCTCCTCCGAGGGGCTGGCCAGTCTCTTCTCCC32800.14087749499197685No Hit
CAACCCTGCATCTTTAGACAAGGCCATGTCCATTCCCATGTGGGTGGACA32730.1405768418014452No Hit
GGTGGCGTGACCTGTAGATGCTGAGGAAGTGTCGGTGACAGGAAGAGGGG32350.1389447244814162No Hit
GGTTTTATCCTCAGCTTCTTCATAGCTGTCTTCATATCAGCATTTCTGAG32180.13821456673298216No Hit
GCAGGTACCCTCAACTGAGTAAGGGACAATGAACAGCCTCTTGGCCATTC32170.13817161627719193No Hit
ATGGCATTGTATGAGAGCATGAGGGAACGCAGGTTGGACAGGTGGGCAAA32020.13752735944033836No Hit
AAAGCAGATCTCAATTAAGCAGACCAGCATTTGCACAGGATCACTCAGGC31800.13658244941295317No Hit
TTGCCTCCTTGGTATGAAACATCTTTCACACAACCCTCAATTGTAGGAGT31550.13550868801819727No Hit
GGTTAGGTAGGAGCTTACTCAGTGCATCTGGAGGATGGAAAACCAATGGC31340.1346067284466023No Hit
CATCTAATTTCCCGGGCCATACCCTGCAGGTACTGGATGATGCTGAGGTT31240.1341772238886999No Hit
CAATGTCCACAAAGATGACCATCTCGTTGGCTGAGGTGTCTGCACAGGCC31110.1336188679634268No Hit
GACAAGCTGGTGTCTGTATTCTACACCATCCTCACTCCCATGCTGAATCC30800.13228740383392948No Hit
GAAATATCCAGTTACTCCTCTAGTCCCATCACCTCGCTTTATGATGTGTA30320.1302257819559981No Hit
CCTGAAGTCTGGCTATGGAAAATATCTTGGTATAAATTCAGATGAACTTG30030.12898021873808124No Hit
GCGGGACCACAATCCTTCGCTTTTCTCCAATGCAAACACCAAGTAGACCT29810.12803530871069604No Hit
AAAGTACTCACTCTCCACACTTGCCCTTTCCTAACACTTTCCTGAGTGCT29400.12627434002329632No Hit
GTGTGATCTGAACCCTTTGCTCAACCTCGCCTGCACTGACACCCATATGC29010.1245992722474771No Hit
CGTGTTGTGCAGTCTCTTTCTGTGCAGGTCGTCCTTCCGGACCAGCACCA28730.12339665948535045No Hit
GTAGTCGCCATGGAGGTAACTGGAATGATACGCGCCGTGGTGGTCTTGGT28590.12279535310428716No Hit
CCTGCATATAGTGGACGAGAAACAGCATATTCCAATGCATCCCTGCTGAT28540.12258060082533596No Hit
GGTTTTCTGCACTGCTTGTAAAACAGACTTGTAGATGCGGAAACTGATGG28100.12069078077056553No Hit
AGGTGACACCATGCCTCTTCCTGTCACTAGCCCTTCCTCAGCATCCACAG27320.11734064521892708No Hit
CATCGTGTTTGGGAGTTTCCTGCTGCTGCTCTTCTCCCTGACCCAGTTCA26760.1149354196946738No Hit
GACGTGTCTAAGGTGGCATTCCTGACCGTGACCCTACACCAAGGCGGAGC26660.11450591513677143No Hit
GCCACCAAATCGGGAGAAGCGGCGGTCTCCGCTGAAGGTGCTGAGCAGCA26460.11364690602096669No Hit
CAGGAACGTACTGGTGAAAACACCGCAGCATGTCAAGATCACAGATTTTG26050.111885937333567No Hit
GCCGCTGACCAAGAATGAGGGGCTGTGGGAGGCAGGGGCTGGTGAGCAGC26010.11171413551040604No Hit
TTAACACTCACAGCACTGGATGGTGGCTCTCCGCCCAGATCTGGCACTGC25940.11141348231987437No Hit
GGCAAGTGGAAAGCCTTCTCCACCTGTGGCTTACACCTCACTGTGGTGTC25920.11132758140829391No Hit
GCGGGACCACAATCCTTCGCTTCTCTCCAATGCAAACACCAAGTAGACCT25630.11008201819037705No Hit
GAAGTGAGCTGCAGTCTCTGCTGATTGCCACGACCTGCCTTCGGGAGCAC25570.1098243154556356No Hit
TGTGTGAATGCAGTCATCTGTATAAAATGTCATCATTACCTTGATCATTT25150.10802039631244567No Hit
CGAGTGCGATGACCAGAGCGACGAGGAGGGCTGCCCCGTGTGCTCCGCCG25150.10802039631244567No Hit
TGCTGGTCAAGGACAATGGCGAGCCTCCGCGCTCGGCCACCGCCACGCTG24750.10630237808083619No Hit
AAGGCACACTTACCACTGTTTTCTTGGCTCCAGTCCAAACCTGAGAGTAG24700.10608762580188501No Hit
GCCCCAAGCTCGTATCCCGGAGGGCAGGGCCAGGGTCTTGGCAGAGCCTT24540.10540041850924121No Hit
ACCAGCAAGATCATGGTATCTGTATGGGCCTTCTTCGCTGTCATATTCCT24450.10501386440712908No Hit
ACCTTACCTTTTTGTCACTGAGGCCGGTCACTTGGTCCACAAATTCCTCT24320.10445550848185602No Hit
CCACCTCACTGTAGTGTCCTTCTACTATGCACCCTTTGCTTATACCTATG24120.10359649936605127No Hit
CCCTCTTTCACTCAGCTTCACTCCACCATGACCAGAGCACCTCTCCTGCT24040.10325289571972938No Hit
TGCTGTAACAAAGGTGACTGTGGCACTCCAAGCCATTCCAGGAGGCAGCC23750.10200733250181251No Hit
CGACCTTATCCAGCCACACGCCACCATGTGCCACACCAGCTGCTCCTCGG23680.10170667931128086No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)